Validated RP-HPLC Method for the Determination of Zolmitriptan – A Serotonin 5-HT Receptor Agonist – Pages 9-14

Validated RP-HPLC Method for the Determination of Zolmitriptan – A Serotonin 5-HT Receptor Agonist – Pages 9-14
M. Mathrusri Annapurna1and Bidyut Nanda2
1Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Rushikonda, Visakhaptanam, Andhra Pradesh, India-530045; 2Department of Pharmaceutical Analysis and Quality Assurance, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India-760010
DOI: http://dx.doi.org/10.6000/1927-5951.2011.01.01.03

Abstract: A simple precise, accurate RP-HPLC method has been developed and validated for analysis of Zolmitriptan (ZLM). The separation and quantization were achieved on a 250 mm reversed phase column with a hydrophilic linkage between silica particles and hydrophobic alkyl chains. The mobile phase was constituted (flow rate 0.8 ml min-1) of eluant A (CH3OH) and eluant B (aqueous tetra butyl ammonium hydrogen sulphate) (pH 3.4; 10 mM) using isocratic elution with UV detection at 224 nm. The method showed good linearity for ZMT in the 1–100 µg mL-1 range with regression equation 15576x ± 99401 and correlation coefficient 0.999 respectively. The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 0.8134 and 0.2687 µg mL-1 respectively. Finally the applicability of the method was validated according to ICH guidelines and can be applicable for the analysis of commercial dosage forms.

Keywords: Zolmitriptan, RP-HPLC, ICH, LOD, LOQ(