Structural Characterization of ZS – 2A: An Antiplasmodial Compound Isolated from Zizyphus spina-christi Root Bark – Pages 48-53

Structural (… )Characterization of ZS – 2A: An Antiplasmodial Compound Isolated from Zizyphus spina-christi Root Bark – Pages 48-53
Bulus Adzu1,2, Abdu Kaita Haruna2, Mohammad Ilyas2, Umar Usman Pateh2, Florence David Tarfa3, Ben Ahmed Chindo1and Karniyus Shingu Gamaniel1
1Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria; 2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria and 3Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria

DOI: http://dx.doi.org/10.6000/1927-5951.2011.01.01.09

Abstract: Zizyphus spina-christi (Rhamnaceae) is a popular medicinal plant that grows wildly in Asia and Tropical Africa.The plant is widely used in ethnomedical practice for the treatment of fever. As a step towards the isolation of biologically active constituents of this plant, we carried out a bioassay guided extraction of the root bark using solvents of varying polarity including, hexane, chloroform, ethylacetate and methanol. An antiplasmodial compound, designated as ZS-2A, was isolated from the chloroform extract and the chemical structure of the compound was characterized using UVvisible, IR, 13C and 1H NMR and thermo-analytical techniques. Our analysis established ZS – 2A as a betulinic acid.

Key Words: ZS-2A, spectroscopy, thermo-analysis, betulinic acid.