Influence of Phytohormones on Callus Indication and Micrpropagation on Rose (Rosa indica L.)

Influence of Phytohormones on Callus Indication and Micrpropagation on Rose (Rosa indica L.)

Pages 9-11

Mehran Ali1, Shahla Karim Baloch1, Nighat Seema3, Shafqat Yaeen4, Arshad Ali Kaleri2, Rameez Raja Kaleri5, Ghulam Shah Nizamani4, Ghulam Farooq Subhapoto1, Mohsin Ali Kaleri1, Faizan Shahani1 and Memona Shahani1

1Department of Biotechnology, Sindh Agriculture University, Tandojam, Pakistan; 2Department of Plant Breeding and Genetics, Sindh Agriculture University, Tandojam, Pakistan; 3Depatment of Agronomy, Sindh Agriculture University, Tandojam, Pakistan; 4Department of Plant Breeding and Genetics, Section Nuclear Institute of Agriculture, Tandojaom, Pakistan; 5Department of Animal Breeding and Genetics, Sindh Agriculture University, Tandojam, Pakistan

https://doi.org/10.6000/1927-5129.2018.14.02

Abstract: Roses (Rosa indica L.) is one of the greatest significant flower collection in the biosphere and have commercial value in decorative, therapeutic and cosmetic trade. Speedy development and construction of disease free plants through in vitro technique have played a dynamic part in broadcast of salable rose cultivars. The five diverse meditations of growth controller such as (MS + 2.00 mg L-1 ), (IBA + 2.00 mg L-1 ), (AA + 30 gL-1 sucrose), (MS + 2.00 mg L-1), (BAP + 3.00 mg L-1 ), (NAA +30 mg L-1 sucrose), (MS + 2.00 mg L-1), (BAP + 3.00 mg L-1 ), ( IAA + 30 mg L-1 sucrose), (MS + 4 mg L-1), (BAP + 2.00 mg L-1), (( IAA + 30 mg L-1 sucrose) and (MS + 3.00 mg L-1 ), (BAP + 3.00 mg L-1), (IAA + 30 mg L-1 sucrose for shoot induction. After establishment of plantlets, stem was cast off as explants foundation for the initiation of callus. It is concluded that the two different concentrations 2.00 and 4.00 mg L-1 of 2 to 4 D were better worked out for callus induction.

Keywords: Influence, phytohormone, callus induction, micropropagation, rose (Rosa indica L.).