The Environmental Effects of Lead Concentrations on Protein and DNA Structures in Epileptic Patients from an Infrared Spectroscopic Study – Pages 56-63

The Environmental Effects of Lead Concentrations on Protein and DNA Structures in Epileptic Patients from an Infrared Spectroscopic Study

Pages 56-63

Jane Anastassopoulou1, Maria Kyriakidou2, Pavlos Nisianakis3, George Papatheodorou4, Michail Rallis5 and Theophile Theophanides2

1International Anticancer Research Institute, 1st km Kapandritiou-Kalamou Road, P.O. Box 22, Kapandriti, 19014 Attiki, Greece; 2National Technical University, Chemical Engineering School, Radiation Chemistry & Biospectroscopy, Zografou Campus, 15780 Athens, Greece; 3Center of Biological Research of Armed Forces, 6 Tax. Veliou, P. Penteli, 15236 Attiki, Greece; 4401 Army General Hospital, 138 Mesogion Ave., 11525 Athens, Greece; 5Department of Pharmacy, Pharmaceutical Technology, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, 15784 Athens, Greece

https://doi.org/10.29169/1927-5129.2019.15.07

Abstract: Fourier transform infrared (FT-IR) and inductively coupled plasma mass spectrometry (ICP-MS) elementary analysis were used to investigate the environmental effects of lead blood serum levels on the life metal ions (Cu2+ and Zn2+), protein secondary structure and DNA structure in epileptic patients. By increasing the lead concentration an increased intensity of the band at 1744 cm-1 was observed due to induced oxidative stress. The shifts of the amide I and amide II bands of the peptide group, -CONH- from 1655 cm-1 and 1550 cm-1, respectively, to lower frequencies is due to the change of protein molecular structure from α-helix to β-sheets. An important change in the spectral region between 1200-900 cm-1, where the phosphates and phosphate-ribose groups of DNA and RNA are absorbing, is suggesting an attack on the DNA backbone as a function of the increase of lead concentration. The characteristic band at 1170 cm-1 could be used as a “marker band” for the damaged DNA backbone structure upon lead exposure. The ICP-MS elementary analysis showed a decrease of the ratio [Cu/Zn] by increasing the lead levels in blood serum is linked to oxidative stress and is confirming the FT-IR data.

Keywords: Infrared spectroscopy, ICP-MS, Environmental pollution, Lead, Epilepsy, oxidative stress.