Semi-Refined Carrageenan Attenuates Lipopolysaccharide-Induced Reactive Oxygen Species Production in Peripheral Blood Mononuclear Cells and Cell Membrane Alterations in Leukocytes – Pages 175-183

Semi-Refined Carrageenan Attenuates Lipopolysaccharide-Induced Reactive Oxygen Species Production in Peripheral Blood Mononuclear Cells and Cell Membrane Alterations in Leukocytes

Pages 175-183

Yevgen Posokhov1,2, Anatolii Onishchenko2,3, Tetyana Chumachenko4, Nataliia Makieieva5, Yuliia Kalashnyk-Vakulenko6, Hanna Polikarpova3, Viktoriia Novikova7, Volodymyr Prokopyuk2,8, Oksana Nakonechna3, Dmytro Chumachenko9, Viktoriya Tkachenko10, Ievgen Meniailov9, Maryna Tkachenko11, and Anton Tkachenko2,3,*

Department of Organic Chemistry, Biochemistry and Microbiology, The National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, Ukraine; 2Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, Ukraine; 3Department of Biochemistry, Kharkiv National Medical University, Kharkiv, Ukraine; 4Department of Epidemiology, Kharkiv National Medical University, Kharkiv, Ukraine; 5Department of Pediatrics No 2, Kharkiv National Medical University, Kharkiv, Ukraine; 6Department of Otorhinolaryngology, Kharkiv National Medical University, Kharkiv, Ukraine; 7Department of Chemistry, Biochemistry, Microbiology and Food Hygiene, State Biotechnological University, Kharkiv, Ukraine; 8Department of Cryobiology of the Reproduction System, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine; 9Department of Mathematical Modelling and Artificial Intelligence, National Aerospace University “Kharkiv Aviation Institute”, Kharkiv, Ukraine; 10D.P. Grynyov Department of Microbiology, Virology and Immunology, Kharkiv National Medical University, Kharkiv, Ukraine; 11L.T. Malaya Department of Internal Medicine No. 2, Clinical Immunology and Allergology, Kharkiv National Medical University, Kharkiv, Ukraine

DOI: https://doi.org/10.29169/1927-5951.2021.11.20

Abstract: Aim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes.

Methods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml), LPS (1 µg/ml), E407a (10 mg/ml) + LPS (1 µg/ml), E407a (50 mg/ml) + LPS (1 µg/ml) and without those compounds (controls) for 2 h in RPMI 1640 medium enriched with 5% fetal bovine serum. ROS generation in PBMCs obtained from the incubated samples was estimated by flow cytometry using 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. The impact of E407a, LPS and their mixture on leukocyte cell membranes was evaluated spectrofluorimetrically using the fluorescent probe 2-(2¢-hydroxy-phenyl)-5-phenyl-1,3-oxazole.

Results: Expectedly, incubation with LPS induced ROS generation in PBMCs and decreased the lipid order of cell membranes in leukocytes. E407a alone was found to alter neither ROS production in PBMCs, nor membrane lipid order in leukocytes. Semi-refined carrageenan partially reduced LPS-mediated ROS overproduction in PBMCs and cell membrane alterations in leukocytes.

Conclusion: E407a attenuates LPS-induced alterations of redox homeostasis in rat PBMCs and LPS-mediated modifications of cell membrane lipid order in leukocytes.

Keywords: Food additive, E407a, 2′,7′-dichlorodihydrofluorescein diacetate, fluorescent probes.