Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation – Pages 40-48

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Pages 40-48

Anton Tkachenko1,2,*, Denys Pogozhykh3, Anatolii Onishchenko1,2, Valeriy Myasoedov4, Leonid Podrigalo5, Vladimir Klochkov6, Tetyana Chumachenko7, Volodymyr Prokopyuk1,8, Svetlana Yefimova6, Galina Gubina-Vakulyck9, Nataliya Kavok6, Dmytro Butov10, Andrii Andrieiev9, Hanna Polikarpova2 and Oksana Nakonechna2

1Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Trinklera st. 6, 61022 Kharkiv, Ukraine; 2Department of Biochemistry, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine; 3Clinic for Hematology, Hemostaseology, Oncology and Stem Cell Transplantation, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; 4Department of Medical Biology, Kharkiv National Medical University, Nauky ave. 4, 61022, Kharkiv, Ukraine; 5Department of Medical Science, Kharkiv State Academy of Physical Culture, Klochkovska str., 99, 61022, Ukraine; 6Yu.V. Malyukin Department of Nanostructured Materials, Institute for Scintillation Materials National Academy of Sciences of Ukraine, Nauky ave. 60, 61072 Kharkiv, Ukraine; 7Department of Epidemiology, Kharkiv National Medical University, Trinklera st. 12, 61022 Kharkiv, Ukraine; 8Department for Cryobiology of the Reproduction System, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv 61015, Ukraine; 9Department of Pathological Anatomy, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine; 10Department of Phthisiology and Pulmonology, Kharkiv National Medical University, Newton st. 145, 61000 Kharkiv, Ukraine

DOI: https://doi.org/10.29169/1927-5951.2021.11.06

Abstract: Gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation.

This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation.

Materials and Methods: Fragments of the small intestine of 8 rats treated orally with a carrageenan-containing food additive E407a at a dose of 140 mg / kg of weight during 2 weeks, 8 animals orally exposed to both E407a and VNPs at a dose of 20 μg / kg of weight during the same period of time, and 8 control rats were stained routinely and immunostained for CD3 and CD68 with the subsequent immunohistochemical scoring. Moreover, analysis of viability and cell death modes of granulocytes was performed by flow cytometry using Annexin V and 7-aminoactinomycin D (7-AAD).

Results: Oral exposure to the food additive E407a resulted in the development of enteritis associated with altered small intestinal morphology, infiltration of the lamina propria with macrophages and T-lymphocytes, and activation of peripheral blood granulocyte apoptosis. VNPs administered against the background of E407a-induced slight intestinal inflammation improved small intestinal morphology, decreased infiltration rate of the immune cells mentioned above without affecting the intensity of granulocyte apoptosis.

Conclusion: Oral administration of VNPs ameliorates E407a-induced enteritis.

Keywords: Carrageenan, food additive E407a, flow cytometry, apoptosis, necrosis, nanoparticles.