Inhibitory Effects of Brazilian Propolis on Tumor Promotion in Two-Stage Mouse Skin Carcinogenesis – Pages 71-76

Inhibitory Effects of Brazilian Propolis on Tumor Promotion in Two-Stage Mouse Skin Carcinogenesis – Pages71-76
Ken Yasukawa1, So Yeon Yu1, Shigetoshi Tsutsumi2, Masahiko Kurokawa3 and Yong K. Park4
1School of Pharmacy, Nihon University, Chiba, Japan; 2Amazon Food Ltd., Tokyo, Japan; 3School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Miyazaki, Japan; 4College of Food Engineering, State University of Campinas, Sao Paulo, Brazil
DOI: http://dx.doi.org/10.6000/1927-5951.2012.02.01.10

Abstract: Propolis is produced by honeybees and has many biological properties, including immunomodulatory, anti-inflammatory, anti-oxidant, anti-bacterial, anti-viral and anti-cancer actions. Five ethanol extracts of Brazilian propolis were tested for inhibition of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. The ethanol extract of Brazilian propolis AF-08 markedly inhibited TPA-induced inflammatory ear edema in mice. This extract suppressed tumor promotion by TPA following initiation with 7,12-dimethylbenz[a]anthracene (DMBA) in mouse skin. Moronic acid, a major component of Brazilian propolis AF-08, markedly inhibited two-stage carcinogenesis by DMBA plus TPA. These results suggest that Brazilian propolis AF-08 contributes to the prevention of cancer.

Keywords: Propolis, moronic acid, cancer chemoprevention, tumor promotion, two-stage carcinogenesis