Health Concerns on Microbiological Quality of Bottled Drinking Water Sold in Dar es Salaam, Tanzania – Pages 17-25

Health Concerns on Microbiological Quality of Bottled Drinking Water Sold in Dar es Salaam, Tanzania- Pages17-25
Kennedy D. Mwambete and Esther K. Sangwa
Department of Pharmaceutical Microbiology, Muhimbili University of Health & Allied Sciences, School of Pharmacy, P.O. Box 65013, Dar es Salaam, Tanzania

Abstract: Background:Dar es Salaam City has over 10 million habitants, who are constantly circumvented by water-borne diseases. The city experiences humid and hot weather throughout a year, which lead to high consumption of bottled drinking water because of being perceived as safer than tap or well water. Waterborne disease outbreaks still create havoc among the city habitants. This study determines heterotrophic plate count (HPC) values in bottled water and performs antimicrobial resistance tests on isolated microorganisms.

Methods: A cross-sectional study was conducted. Fifty-four samples of bottled water were randomly purchased from shops, supermarkets and streetvendors. HPC values were determined and microbial contaminants identified. The disk diffusion method was employed for testing antimicrobial resistance of microbial contaminants against four widely used antibiotics.

Results: HPC values were variable (4.7 x102 to 7.0 x105 cfu/ml). Majority (86.7%) of analyzed samples revealed high HPC values. Four bacterial species were isolated and identified from 47 samples. Predominant bacterial contaminants were Brevundimonasvesicularis (54.5%), while Pseudomonas thomasii was only isolated from one brand A sample.Brand E samples had the highest HPC values (2.2 x 10to 2.16 x105cfu/ml) while F samples were free from microbial contaminants. A total of 21 (46.7%) bacterial isolates were resistant to commonly used antibiotics namely ciprofloxacin, ampicillin, cotrimoxazole and chloramphenicol.

Conclusion: Of 54 analyzed bottled water samples, 47 had high HPC values. High rates of antibacterial resistance was exerted against ciprofloxacin (68.2%) and ampicillin (56%). This calls for responsible authorities to impose more stringent measures on in-process quality control among bottled water producers and indefatigably conduct post- production surveillance to avert the endemic water-borne outbreaks resulting from consumption of such products.

Keywords: Heterotrophic plate count values, microbial contaminants, antibiotic resistance profiles