Studies on Cisplatin and Etoposide Treated Liver Protein Extract Under SDS-PAGE Gel Separation – Pages 26-29

Studies on Cisplatin and Etoposide Treated Liver Protein Extract Under SDS-PAGE Gel Separation- Pages26-29
Pratibha Kamble1and Dayanand A.Bhiwgade2
1Department of LifeSciences, University for Mumbai, Kalina, Santacruz (E) 400098, Mumbai, India; 2Department of Biological Sciences, D.Y. Patil University, Belapur, Navi Mumbai, India

AbstractCytotoxic agents has been widely used for the treatment various human malignancies. Cisplatin and etoposide are chemotheraupeutic agents which are used for treatment of metastatic cancers, solid tumors and neoplasm. The drug is known to exert its toxic effect on hepatic and renal tissue by altering its antioxidant response of these organs. In the present study both the drugs Cisplatin and etoposide were analyzed for its effects on hepatic protein status. Cisplatin has shown to cause consequential alterations in protein levels as compared to etoposide treated hepatic tissue. The protein with molecular weight 44.46, 13.8, 51.2 kd was downregulated and other ranging from 16.98-38.9 kd did not show any expression as compared to controls. In addition etoposide studies revealed overexpression in protein level ranging from molecular weight 14.45-33.11 kd which might suggest significant role in organ protection. Some of protein bands ranging from 25.12 to 51.29 kd showed similar pattern of expression compared to controls. Present studies might provide us clues of important role of these protein in combating free radical induced oxidative stress on the tissue.

Keywords: Cisplatin, Liver, Etoposide, free radical, Oxidative Stress.