In Vitro Cytotoxic Activity of Isostichopus badionotus, a Sea Cucumber from Yucatan Peninsula Coast – Pages 183-186

In Vitro Cytotoxic Activity of Isostichopus badionotus, a Sea Cucumber from Yucatan Peninsula Coast
Pages 183-186
Aida R. Pérez-Espadas, María J. Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cárdenas, María E. Morales-Rubio, Lorena V. León-Deniz, Jaqueline Canul-Canché and Leovigildo Quijano
DOI: http://dx.doi.org/10.6000/1927-5951.2014.04.03.3
Published: 12 August 2014

Abstract: The in vitro cytotoxic activity of hexane, ethyl acetate and butanol extracts of the sea-cucumber Isostichopus badionotus (Holothuroidea) was tested against normal cells (Vero), human cervical carcinoma (HeLa) and breast adenocarcinoma (MCF-7 andMDA-MB-231) ATCC cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Hexane extracts from body walls and viscera showed high cytotoxic activity against HeLa cells (IC50’s = 48.5 and 42.5 µg mL-1, respectively), while the ethyl acetate extract of body walls was considered low active (IC50 = 98.3μg mL-1). In addition, the body walls hexane extract showed a good selectivity index value of 12.0.

Keywords: Holothurian, organic extracts, selectivity index, HeLa cells.