Propagation of Blue Honeysuckles (Lonicera caerulea L.) in In Vitro Culture


 In vitro, Lonicera caerulea L., media, micropropagation, plant growth regulators.

How to Cite

Krupa-Małkiewicz Marcelina, & Ochmian Ireneusz. (2014). Propagation of Blue Honeysuckles (Lonicera caerulea L.) in In Vitro Culture. Journal of Basic & Applied Sciences, 10, 164–169.


The aim of this study was to develop micropropagation protocol for Lonicera caerulea L. Clone 44, Clone 46 and Br?zowa, three important invasive woody horticultural plants. Actively growing shoots from the shrubs grown in the field were used for initiation of culture. Shoots were surface sterilized with ethanol, then with sodium hypochlorite and mercury sulfate. MS medium supplemented with cytokinin BAP at concentrations of 1.0 - 4.0 mg·dm-3 had no statistically significant effect on the shoot initiation of selected blue honeysuckle genotypes. Multiplication rate varied depending on the genotype and plant growth regulator concentrations. The highest number of microshoots produced per explant of Clone 44 and Clone 46 was obtained at using 2.0 mg·dm-3 BAP, while of cultivar Br?zowa – 1.0 mg·dm-3 BAP. Shoots were rooted in vitro in the presence of IBA and IAA. Microshoots have rooted differently depending on the treatment and genotype. In the case of Clone 4458% rooting was achieved at 2.5 mg·dm-3 IBA and MS basal nutrient medium treatment.


Palacios N, Christou P, Leech M. Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultured stem explants. Plant Cell Rept 2002; 20:808-813.

Karhu ST. Axillary shoot proliferation of blue honeysuckle. Plant Cell, Tissue and Organ Cult 1997; 48:195-201.

Dziedzic E. Propagation of blue honeysuckle (Lonicera caerulea var. kamtchatica Pojark.) in in vitro culture. J Fruit Ornam Plant Res 2008; 16: 93-100.

Hui JX, Wen SCh, Hua ZY, Ming LX. Comparative study on different methods for Lonicera japonica Thunb. micropropagation and acclimatization. J Med Plant Res 2012; 6: 4389-4393.

Georges D, Chenieux JC, Ochatt SJ. Plant regeneration from aged-callus of the woody ornamental species Lonicera japonica cv. Hall’s Prolific. Plant Cell Rep 1993; 13: 91-94.

Cambecèdes J, Duron M, Decourtye L. Adventitious bud regeneration from leaf explants of the shrubby ornamental honeysuckle, Lonicera nitida Wils cv. Maigrün: effects of thidiazuron and 2,3,5-triiodobenzoic acid. Plant Cell Rep 1991; 10: 471-474.

Ochatt SJ. Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maigrün. Plant Cell, Tissue Organ Cult 1991; 25: 161-167.

Sedlák J, Paprštein F. In vitro propagation of blue honeysuckle. Hort Sci 2007; 34: 129-131.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962; 15: 473-497.

Yildiz M, Er C. The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax (Linum usitatissimum). Naturwissenschaften 2002; 89: 259-261.

Tiwari AK, Tripathi S, Lal M, Mishra S. Screening of some chemical disinfectants for media sterilization during in vitro micropropagation of sugarcane. Sugar Tech 2012; 14: 364-369.

Osburn LD, Yang X, Li Y, Cheng Z-M. Micropropagation of Japanese honeysuckle (Lonicera japonica) and Amur honeysuckle (L. maackii) by shoot tip culture. J Environ Hort 2009; 27: 195-199.

Karhu ST. Rooting of blue honeysuckle microshoots. Plant Cell, Tissue and Organ Cult 1997; 48: 153-159.

Suzuki T, Uenohata M, Oosawa K. Polyploidy breeding of blue honeysuckle and black chokeberry by utilizing in vitro-cultures treated with colchicine. Acta Hort 2006; 760: 389-396.

Karhu ST. Performance of Lonicera microcutting as affected by mineral nutrients and genotype. Acta Hort 2003; 616: 181-183.

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Copyright (c) 2014 Krupa-Małkiewicz Marcelina, Ochmian Ireneusz