The Proliferation and Migration-Enhancing Effects of Vitronectin in SMMC 7721 Cells: A Pilot Study


  • Wei Zhu Guangzhou Center for Disease Control and Prevention, Guangzhou, China
  • Konghe Hu The Affiliated Yue Bei People's Hospital of Shantou University Medical College, Shaoguan, China
  • WenXue Li Guangzhou Center for Disease Control and Prevention, Guangzhou, China
  • Guangyu Yang Guangzhou Center for Disease Control and Prevention, Guangzhou, China
  • Jianling Chen Guangdong Prevention and Treatment Center for occupational Disease
  • Chuanxi Fu Guangzhou Center for Disease Control and Prevention, Guangzhou, China
  • Qiansheng Hu Sun Yet-sen University, Guangzhou, China



 Vitronectin, Hepatocellular Carcinoma, Proliferation, Tumor Cell Migration.


To understand the effects of Vitronectin on cell proliferation and migration in the cell line of hepatocellular carcinoma, SMMC 7721, the effects of Vitronectin on cell proliferation rate or on the prevention of the cells from the apoptotic stimuli were appraised with WST-1 assay; and the morphology of b-Tubulin was observed with con-focal microscope. The effect on migration was detected with transwell chamber. The results show that Vitronectin helps the cells adhere to Petri dish as well as the sustaining of the morphology of b-Tubulin. Vitronectin could enhance the proliferation rate of SMMC 7721 with the concentration-effect mode, and could protect the cells from the stimuli of apoptosis inducer. As to cell migration, the results show that Vitronectin enhance cell migration across the membrane of transwell chamber. According the results, the conclusion could be made that Vitronectin might play important roles in the following biological effects, such as sustaining the morphology of the tumor cells, enhancing the proliferation as well as the migration.


Ascha MS, Hanouneh IA, Lopez R, Tamimi TA, Feldstein AF, Zein NN. The incidence and risk factors of hepatocellular carcinoma in patients with nonalcoholic steatohepatitis. Hepatology 2010; 51: 1972-78.

Xu Y, Liu L, Liu J, et al. A potentially functional polymorphism in the promoter region of miR-34b/c is associated with an increased risk for primary hepatocellular carcinoma. International journal of cancer. J Int du Cancer 2011; 128: 412-17.

Liu JM, Li FM, Liu ZB, et al. 8-Quinolineboronic acid as a potential phosphorescent molecular switch for the determination of alpha-fetoprotein variant for the prediction of primary hepatocellular carcinoma. Anal Chim Acta 2010; 663: 184-89.

Cui Z, Yu X, Guo L, et al. Combined analysis of serum alpha-fetoprotein and MAGE-A3-specific cytotoxic T lymphocytes in peripheral blood for diagnosis of hepatocellular carcinoma. Disease Markers 2013; 35: 915-23.

Barnes D, Wolfe R, Serrero G, McClure D, Sato G. Effects of a serum spreading factor on growth and morphology of cells in serum-free medium. J Supramol Struct 1980; 14: 47-63.

Jaskiewicz K, Chasen MR, Robson SC. Differential expression of extracellular matrix proteins and integrins in hepatocellular carcinoma and chronic liver disease. Anticancer Res 1993; 13: 2229-37.

Edwards S, Lalor PF, Tuncer C, Adams DH. Vitronectin in human hepatic tumours contributes to the recruitment of lymphocytes in an alpha v beta3-independent manner. Br J Cancer 2006; 95: 1545-54.

Nejjari M, Hafdi Z, Gouysse G, et al. Expression, regulation, function of alpha V integrins in hepatocellular carcinoma: an in vivo and in vitro study. Hepatology 2002; 36: 418-26.

Zhong Wu X, Honke K, Long Zhang Y, Liang Zha X, Taniguchi N. Lactosylsulfatide expression in hepatocellular carcinoma cells enhances cell adhesion to vitronectin and intrahepatic metastasis in nude mice. Int J Cancer 2004; 110: 504-10.

Uhm JH, Dooley NP, Kyritsis AP, Rao JS, Gladson CL. Vitronectin, a glioma-derived extracellular matrix protein, protects tumor cells from apoptotic death. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research 1999; 5: 1587-94.

Shih YT, Wang MC, Peng HH, et al. Modulation of chemotactic and pro-inflammatory activities of endothelial progenitor cells by hepatocellular carcinoma. Cell Signal 2012; 24: 779-93.

Primiceri E, Chiriaco MS, Dioguardi F, et al. Automatic transwell assay by an EIS cell chip to monitor cell migration. Lab Chip 2011; 11: 4081-86.

Serini S, Piccioni E, Merendino N, Calviello G. Dietary polyunsaturated fatty acids as inducers of apoptosis: implications for cancer. Apoptosis: An International Journal on Programmed Cell Death 2009; 14: 135-52.

Wang Y, Lee CG. MicroRNA and cancer--focus on apoptosis. J Cell Mol Med 2009; 13: 12-23.

Kim J, Hong SY, Park HS, Kim DS, Lee W. Structure and function of RGD peptides derived from disintegrin proteins. Mol Cells 2005; 19: 205-11.

Bellone M, Cocco E, Varughese J, et al. Expression of alphaV-integrins in uterine serous papillary carcinomas; implications for targeted therapy with intetumumab (CNTO 95), a fully human antagonist anti-alphaV-integrin antibody. International Journal of Gynecological Cancer: Official Journal of the International Gynecological Cancer Society 2011; 21: 1084-90.

Mooney A, Jackson K, Bacon R, et al. Type IV collagen and laminin regulate glomerular mesangial cell susceptibility to apoptosis via beta(1) integrin-mediated survival signals. Am J Pathol 1999; 155: 599-606.

Fan S, Meng Q, Xu J, et al. DIM (3,3'-diindolylmethane) confers protection against ionizing radiation by a unique mechanism. Proc Natl Acad Sci USA 2013; 110: 18650-55.

Wong CP, Hsu A, Buchanan A, et al. Effects of sulforaphane and 3,3'-diindolylmethane on genome-wide promoter methylation in normal prostate epithelial cells and prostate cancer cells. PloS One 2014; 9: e86787.

Lee SH, Min KW, Zhang X, Baek SJ. 3,3'-diindolylmethane induces activating transcription factor 3 (ATF3) via ATF4 in human colorectal cancer cells. J Nutr Biochem 2013; 24: 664-71.

Shorey LE, Hagman AM, Williams DE, Ho E, Dashwood RH, Benninghoff AD. 3,3'-Diindolylmethane induces G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia cells. PloS One 2012; 7: e34975.

Zhu W, Li W, Yang G, Zhang Q, Li M, Yang X. Indole-3-carbinol inhibits nasopharyngeal carcinoma. Int J Toxicol 2010; 29: 185-92.

Kisiday JD, Kurz B, DiMicco MA, Grodzinsky AJ. Evaluation of medium supplemented with insulin-transferrin-selenium for culture of primary bovine calf chondrocytes in three-dimensional hydrogel scaffolds. Tissue Eng 2005; 11: 141-51.




How to Cite

Wei Zhu, Konghe Hu, WenXue Li, Guangyu Yang, Jianling Chen, Chuanxi Fu, & Qiansheng Hu. (2014). The Proliferation and Migration-Enhancing Effects of Vitronectin in SMMC 7721 Cells: A Pilot Study. Journal of Basic & Applied Sciences, 10, 205–211.