Hydrocarbon degrading bacteria were isolated from the petrol contaminated soil of Karachi to determine their biodegradation capabilities of aromatic hydrocarbons such as xylene, phenanthrene, naphthalene, biphenyl and anthracene. Twelve bacterial strains were isolated by culture enrichment technique in Bushnell Hass medium in the presence of petrol. Hydrocarbon degradation capabilities of bacterial strains were assessed by means of enumeration using spread-plate technique. Current study revealed that all of the twelve isolated bacterial strains were able to degrade aromatic hydrocarbons, particularly Pseudomonas sp. SA044, degraded all the tested five aromatic hydrocarbons while Burkholderia sp., Ralstonia sp., Stenotrophomonas sp., Micrococcus sp. and Staphylococcus sp. degraded three or more aromatic hydrocarbons.Naphthalene and phenanthrene were the most degraded aromatic hydrocarbons.
Chikere CB, Okpokwasili GC, Chikere BO. Monitoring of microbial hydrocarbon remediation in the soil. Biotechnology 2011; 1: 117-138.
Atlas RM, Philp J. Bioremediation: applied microbial solutions for real-world environmental cleanup. American Society for Microbiology (ASM) Press 2005; 78-105
Pawar RM. The effect of soil pH on degradation of polycyclic aromatic hydrocarbons (PAHs) 2012. http://uhra.herts.ac.uk/ bitstream/handle/2299/8965/08177224%20Pawar%20Rakesh%20-%20finalPhD%20submission.pdf?sequence=1
Shukor MY, Dahalan FA, Jusoh AZ, Muse R, Shamaan NA, Syed MA. Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site. Journal of Environmental Biology 2009; 30(1): 145-150.
Samaei MR, Mortazavi SB, Bakhshi B, Jafari AJ. Isolation and characterization of bacteria degrading n-hexadecane from soil.International Proceedings of Chemical, Biological and Environmental Engineering (IPCBEE) 2012; 40.
Pepi M, Cesa`ro A, Liut G, Baldi F. An Antarctic psychrotrophic bacterium Halomonassp. ANT-3b, growing on n-hexadecane, produces a new emulsyfying glycolipid. FEMS Microbial Ecology 2005; 53: 157-166. http://dx.doi.org/10.1016/j.femsec.2004.09.013
Toledo FL, Calvo C, Rodelas B, Lopez JG. Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. Systematic and Applied Microbiology 2006; 29: 244-252. http://dx.doi.org/10.1016/j.syapm.2005.09.003
Panda SK, Kar RN, Panda CR. Isolation and identification of petroleum hydrocarbon degrading microorganisms from oil contaminated environment. International Journal of Environmental Sciences 2013; 3: 5.
Surridge AKJ. Denaturing gradient gel electrophoresis characterisation of microbial communities in polycyclic aromatic hydrocarbon and polychlorinated biphenyl contaminated soil 2007. http://upetd.up.ac.za/thesis/ available/etd-05282007-123145/unrestricted/00front.pdf
Coitinho JB, Costa DMA, Guimara˜es SL, Miranda de Go´es A, Nagem RAP. Expression, purification and preliminary crystallographic studies of NahF, a salicylaldehyde dehydrogenase from Pseudomonas putida G7 involved in naphthalene degradation. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2012; 68: 93-97. http://dx.doi.org/10.1107/S174430911105038X
Hemalatha S, Veeramanikandan P. Characterization of Aromatic Hydrocarbon Degrading Bacteria from Petroleum Contaminated Sites. Journal of Environmental Protection 2011; 2: 243-254. http://dx.doi.org/10.4236/jep.2011.23028
Chauhan A, Rahman F, John G, Oakeshott, Jain RK. Bacterial metabolism of polycyclic aromatic hydrocarbons: strategies for bioremediation. Review of Indian Journal of Microbiology 2008; 48: 95-113. http://dx.doi.org/10.1007/s12088-008-0010-9
Coral G, Karagoz S. Isolation and characterization of phenanthrene-degrading bacteria from a petroleum refinery soil. Annals of Microbiology 2005; 55(4): 255-259.
Daane LL, Harjono I, Zylstra GJ, Haggblom MM. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants. Applied and Environmental Microbiology 2001; 67(6): 2863-2691. http://dx.doi.org/10.1128/AEM.67.6.2683-2691.2001
Kiyohara H, Nagao K, Yana K. Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Applied and Environmental Microbiology 1982; 43: 454-457.
Rasool SA, Ijaz S, Subhan SA. Hydrocarbon degradation by marine bacteria: Screening and genetic manipulation. Pakistan Journal of Botany 2003; 35(1): 37-44.
Johnsen AR, Bendixen K, Karlson U. Detection of microbial growth on polycyclic aromatic hydrocarbons in microtiter plates by using the respiration indicator WST-1. Applied and Environmental Microbiology 2002; 68(6): 2683-2689. http://dx.doi.org/10.1128/AEM.68.6.2683-2689.2002
Subathra MK, Immanuel G, Suresh AH. Isolation and identification of hydrocarbon degrading bacteria from Ennore creek. Bioinformation 2013; 9(3): 150-157. http://dx.doi.org/10.6026/97320630009150
Chaudhary VK, Borah D. Isolation and molecular characterization of hydrocarbon degrading bacteria from tannery effluent.International Journal of Plant Animal and Environmental Sciences 2011; 1(2): 36-49
Khan JA, Rizvi SHA. Isolation and characterization of micro-organism from oil contaminated sites. Advances in Applied Science Research 2011; 2(3): 455-460.
Obuekwe IS, Semple KT. Impact of Al and Fe on the development of phenanthrene catabolism in soil. Journal of Soils and Sediments 2013; 13(9): 1589-1599. http://dx.doi.org/10.1007/s11368-013-0759-2
Kafilzadeh F, Pour FH. Degradation of naphthalene, phenanthrene and pyrene by Pseudomonas sp. and Corynebacteriumsp. in the landfills.International Journal of Biosciences 2012; 2(9): 77-84.
Ahmed F, Hasibullah M, Ferdouse J, Anwar MN. Microbial degradation of petroleum hydrocarbons. Bangladesh Journal of Microbiology 2010; 27: 10-13.
Lopez MEM, Rodriguez-Casasola MT, Rios-Leal E, Esparza-Garcia F, Chavez-Gomez B, Rodriguez-Vazquez R, Barrera-Cortesa J. Fungi and bacteria isolated from two highly polluted soils for hydrocarbon degradation. Acta Chimica Slovenica 2007; 54: 201-209.
Zhao B, Wang H, Mao X, Li R. A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons. Letters in Applied Microbiology 2009; 49: 408-410. http://dx.doi.org/10.1111/j.1472-765X.2009.02668.x
Nikhil T, Deepa V, Rohan G, Satish B. Isolation, characterization and identification of diesel engine oil degrading bacteria from garage soil and comparison of their bioremediation potential. International Research Journal of Environment Sciences 2013; 2(2): 48-52.
Obayori OS, Salam LB. Degradation of polycyclic aromatic hydrocarbons: Role of plasmids. Scientific Research and Essays 2010; 5(25): 4093-4106.
Neeraja P, Madhusudhan NCH, Shanker S, Ravikumar M. Isolation and characterization of anthracene degrading bacteria from soil contaminated with petroleum wastes. International Journal of Research in Pharmaceutical Sciences 2013; 4(3): 380-385.
Morzik A, Piotrowska-Seget Z, Labuzek S. Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons. Polish Journal of Environmental Studies 2003; 12(1): 15-25.
Kiyohara H, Torigoe S, Kaida N, Asaki T, Iida T, Hayashi H, Takizawa N. Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82. Journal of Bacteriology 1994; 176(8): 2439-2443.
Jyothi K, Babu KS, Clara NK, Kashyap A. Identification and isolation of hydrocarbon degrading bacteria by molecular characterization. Helix 2012; 2: 105-111.
Chikere CB, Okpokwasili GC, Ichiakor BO. Characterization of hydrocarbon utilizing bacteria in tropical marine sediments. African Journal of Biotechnology 2009; 8(11): 2541-2544.
Srujana K, Khan AB. Isolation and characterization of polyaromatic hydrocarbon degrading soil microbes from automobile workshop sediments. Journal of Environmental Science and Technology 2012 ; 5(1): 734-83.
Dongfeng Z, Weilin W, Yunbo Z, Qiyou L, Haibin Y, Chaocheng Z. Study on isolation, identification of a petroleum hydrocarbon degrading bacterium Bacillus fusiformissp. and influence of environmental factors on degradation efficiency. Environment Protection 2011; 13(4): 74-82.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2015 Talat Yasmeen Mujahid, Abdul Wahab, Safia Hashim Padhiar, Syed Abdus Subhan, Muneera Naz Baloch , Zaid A. Pirzada