In-Vitro Antileishmanial Activity of Raphanus sativus L. var. caudatus


  • Ishrat Younus Faculty of Pharmacy, Hamdard University, Karachi, Pakistan
  • Afshan Siddiq Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan



 Leishmaniasis, Raphanus sativus L. var. caudatus, ethanolic extract.


Leishmaniasis is a major public health problem causing significant morbidity and mortality around the world specially Asia, Africa and Latin America. The present preliminary in-vitro study reports antileishmanial potential of Raphanus sativus L. var. caudatus. Various concentrations of ethanolic extract of plant were used. The tested extract showed noteworthy inhibition (concentration – dependant) of Leishmania promastigotes. The IC50 of extract was found to be 39 μg/ml. The results of present study could be helpful in future for antileishmanial therapy.


Gazanion E, Vergnes B, Seveno M, Garcia D, Oury B, Ait-Oudhia K, et al. In vitro activity of nicotinamide/ antileishmanial drug combinations. Parasitol Int 2011; 60(1): 19-24.

Berman J. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years. Clin Infect Dis 1997; 24(4): 684-703.

Olliaro P, Bryceson A. Practical progress and new drugs for changing patterns of leishmaniasis. Parasitology today 1993; 9(9): 323-8.

Khare C. Indian Medicinal Plants: an illustrated dictionary Library of congress Control Number: 2007922446 ISBN: 978-0-387-70637-5 Springer-Verlag Berlin. Heidelberg, Springer science+ Business Media, LLC; 2007.

Abdou I, Abou-Zeid A, El-Sherbeeny M, Abou-El-Gheat Z. Antimicrobial activities of Allium sativum, Allium cepa, Raphanus sativus, Capsicum frutescens, Eruca sativa, Allium kurrat on bacteria. Qualitas Plantarum et Materiae Vegetabiles 1972; 22(1): 29-35.

Terras FR, Goderis IJ, Van Leuven F, Vanderleyden J, Cammue BP, Broekaert WF. In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant physiology 1992; 100(2): 1055-8.

Kim KH, Moon E, Kim SY, Choi SU, Lee JH, Lee KR. 4-Methylthio-butanyl derivatives from the seeds of Raphanus sativus and their biological evaluation on anti-inflammatory and antitumor activities. J Ethnopharmacol 2014; 151(1): 503-8.

Vargas S R, Perez G R, Perez G S, Zavala S M, Perez G C. Antiurolithiatic activity of Raphanus sativus aqueous extract on rats. J Ethnopharmacol 1999; 68(1-3): 335-8.

Takaya Y, Kondo Y, Furukawa T, Niwa M. Antioxidant constituents of radish sprout (kaiware-daikon), Raphanus sativus L. J Agric Food Chem 2003; 51(27): 8061-6.

Gutiérrez RMP, Perez RL. Raphanus sativus (Radish): their chemistry and biology. The Scientific World Journal 2004; 4: 811-37.

Duke J, Ayensu E. Medicinal Plants of China. Medicinal Plants of the World. Vol. 1. Algonac, MI. reference publications, Inc; 1985.

Bown D. The Royal Horticultural Society encyclopedia of herbs & their uses: Dorling Kindersley Limited; 1995.

Davey MR, Anthony P. Plant cell culture: essential methods: John Wiley & Sons; 2010.

Habtemariam S. In vitro antileishmanial effects of antibacterial diterpenes from two Ethiopian Premna species: P. schimperi and P. oligotricha. BMC Pharmacol 2003; 3(1): 6.

Choudhary MI, Yousaf S, Ahmed S, Yasmeen K. Antileishmanial physalins from Physalis minima. Chemistry & biodiversity 2005; 2(9): 1164-73.

Rates SMK. Plants as source of drugs. Toxicon 2001; 39(5): 603-13.

Shukla S, Chatterji S, Yadav DK, Watal G. Antimicrobial efficacy of Raphanus sativus root juice. Int J Pharm Pharm Sci 2011; 3: 89-92.

Rani I, Akhund S, Abro H. Antimicrobial potential of seed extract of Raphanus sativus. Pakistan Journal of Botany 2008; 40(4): 1793-8.

Terras F, Schoofs H, De Bolle M, Van Leuven F, Rees SB, Vanderleyden J, et al. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 1992; 267(22): 15301-9.

Beevi SS, Mangamoori LN, Dhand V, Ramakrishna DS. Isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from Raphanus sativus L. Foodborne Pathog Dis 2009; 6(1): 129-36.

Umamaheswari M, Ajith MP, Asokkumar K, Sivashanmugam T, Subhadradevi V, Jagannath P, et al. In vitro angiotensin converting enzyme inhibitory and antioxidant activities of seed extract of Raphanus sativus Linn. Central European Journal of Experimental Biology 2012; 1: 11-7.

Lugast A, Hovari J. Flavonoid aglycons in foods of plant origin I. Vegetables. Acta Alimentaria 2000; 29(4): 345-52.

Mittra B, Saha A, Chowdhury AR, Pal C, Mandal S, Mukhopadhyay S, et al. Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II-mediated kinetoplast DNA cleavage leading to apoptosis. Mol Med 2000; 6(6): 527.




How to Cite

Ishrat Younus, & Afshan Siddiq. (2016). In-Vitro Antileishmanial Activity of Raphanus sativus L. var. caudatus. Journal of Basic & Applied Sciences, 12, 242–244.



Pharmaceutical Sciences