Pathogenesis and Varietal Screening for Panama Wilt Disease of Banana in Pakistan


Panama wilt, Fusarium oxysporum f. sp. cubense, pathogenesis, screening, Cavendish varieties.

How to Cite

Nasreen Sultana, Khalil Ahmed Khanzada, Jawed Akhtar, & Syed Asim Rehan Kazmi. (2019). Pathogenesis and Varietal Screening for Panama Wilt Disease of Banana in Pakistan. Journal of Basic & Applied Sciences, 15, 1–5.


Banana is the world largest fruit crop with an annual production of 114 million tonnes and Pakistan produces 154,800 tons annually. Panama wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is the most widespread and destructive banana diseases worldwide. In Pakistan Panama disease was first recorded in 2012 in few banana fields of district Thatta and Hyderabad in the Sindh. The infection processes and mechanisms of pathogenesis of Foc in relation to symptoms expression and evaluation of Cavendish varieties for panama wilt of banana were studied. Pathogenesis was confirmed by establishing Koch’s postulates. The experiment was to evaluate occurrences of necrotic and wilting symptoms and the disease intensity on banana suckers of Cavendish cultivar, two months plantlets were artificially inoculated with 106 conidia/ml suspension of Foc. Five tested Cavendish varieties viz., w11, G9, Basari, Pisang and BT10 showed low to high average of score of wilting and necrotic symptoms. Based on the calculation of disease intensities, all Cavendish varieties tested were identified as susceptible to Foc infection.


Annonymous, 2019. Banana-Pakistan.Com. Available from

Annonymous, 2017. Banana market review - Preliminary results for 2017. Available from est/est-commodities/bananas/en

Bentley S, Moore N, Pattemore J, Anderson J, Pegg K. A DNA-based diagnostic test for ‘tropical’ race 4 of Fusarium wilt of banana. Infomusa 2001; 10: 9-10.

Cachinero JM, Hervas A, Jimenez-Diaz RM, Tena M. Plant defence reactions against Fusarium wilt in chickpea induced by incompatible race 0 of Fusarium oxysporum f.sp. ciceris and non-host isolates of F. oxysporum. Plant Pathol 2002; 51: 765-776.

Epp D. Somaclonal variation in banana: a case study with Fusarium wilt. In: Persley GJ, De Langhe EA (eds). Banana and Plantain Breeding Strategies. Canberra: ACIAR Publ. 1987.p 140-150.

FAOSTAT, 2017. Available from

García-Bastidas F, Ordóñez N, Konkol J, Al-Qasim M, Naser Z, Abdelwali M, Salem N, Waalwijk C, Ploetz RC, Kema GHJ. First report of Fusarium oxysporum f. sp. cubense Tropical Race 4 associated with panama disease of banana outside Southeast Asia. Plant Dis 2013; 98: 694-694.

Gregory PH. Spore Trapping. In: Johnson A, Booth C (eds) Plant Pathologist’ pocketbook. Slough: CMI. 1983; pp. 328-331.

Hadipoentyanti E, Ratnadewi D, Solihat L. Variabilitas genetic berbagai varietas abaka (Musa textilis Nee) dan kerabat liar melalui analisis RAPD. Zuriat 2001; 12: 93-106.

Li CY, Yi GJ, Chen S, Sun QM, Zuo CW, Huang BZ, Wei YR, Huang YH, Wu YL, Xu LB, Hu CH. Studies on some of the early events in the Fusarium oxysporum-Musa interaction. Acta Horticulture 2011; 897: 305-312.

Mak C, Mohamed AA, Liew KW, Ho YW. Early screening technique for Fusarium wilt resistance in banana micropropagated plants. In: Jain SM, Swennen R (eds). Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Enfield: Sci.Publ, Inc. 2004; pp. 11-20.

Molina AB. Estado de la incidencia en Asia del marchitamiento por Raza 4 tropical de Fusarium en el cultivo del banano. In Reunión de Grupos de Interés sobre los Riesgos de la 27 Raza 4 tropical de Fusarium, BBTV y otras Plagas de Musáceas, OIRSA (San Salvador, El Salvador). Resúmenes 2009; p. 71.

Moore NY, Bentley S, Pegg KG, Jones DR. Fusarium wilt of banana. Musa disease fact sheet no. 5. INIBAP 1995.

Muhammad Hussain AI, Khanzada KA, Kumar L, Ali M, Yasmin T, Hyder MZ. Molecular characterization of Fusarium oxysporum f. sp. cubense (foc) tropical race 4 causing panama disease in cavendish banana in Pakistan. Pak J Agric Sci 2017; 54: 1-8.

Nasir N, Pittaway PA, Pegg KG. A pilot study investigating the complexity of Fusarium wilts of bananas in West Sumatra, Indonesia. Aust J Agric Res 1999; 50: 279-1283.

Ordonez N, Garcia FA, Laghari H, Akkary M, Harfouche EN, Al Awar BN, Kema GHJ. First report of Fusarium oxysporum f. sp. cubense tropical race 4 causing panama disease in Cavendish bananas in Pakistan and Lebanon. Plant Dis 2015; 99: 1448.

Pegg KG, Moore NY, Sorensen S. Fusarium wilt in the Asian Pacific Region. In: Dalam International Symposium on Recent Developments in Banana Cultivation Technology. TBRI 1993; pp. 255-269.

Pietro AD, Madrid MP, Caracuel Z, Delgado-Jarana J, Roncero MI. Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Mol Plant Pathol 2003; 5: 315-325.

Purwati RD, Nurul Hidayah, Sudjindro, Sudarsono. Inoculation Methods and Conidial Densities of Fusarium oxysporum f.sp. cubense in Abaca. HAYATI Journal of Biosciences 2008; 15: 1-7.

Stover RH, Simmond NW. Bananas. 3rd edition. Longman Scientific and Technical New York 1987.

Syed RN, Lodhi AM, Jiskani MM, Rajput KI, Khaskheli MA, Khanzada MA, Rajput NA, Maitlo SA, Rajput AQ. First report of panama wilt disease of banana caused by Fusarium oxysporum f. sp. cubense in Pakistan. J Plant Pathol 2015; 97: 209-220.

Yusnita, Sudarsono. Metode inokulasi dan reaksi ketahanan 30 genotipe kacang tanah terhadap penyakit busuk batang Sclerotium Hayati 2004; 11: 53-58.

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