Effect of Ethanolic Extract of Seeds of Solanum torvum in Acetic Acid induced Ulcerative Colitis in Male Wistar Rats


Acetic acid, Solanum torvum, Ulcerative colitis, Antioxidants, Histopath.

How to Cite

N.S.R. Bhargavi, Sushma Poojari, & Mahalaxmi Mohan. (2019). Effect of Ethanolic Extract of Seeds of Solanum torvum in Acetic Acid induced Ulcerative Colitis in Male Wistar Rats. Journal of Basic & Applied Sciences, 15, 64–72. https://doi.org/10.29169/1927-5129.2019.15.08


Objective: To elucidate the protective effect of ethanolic extract of dried seeds of Solanum torvum (S. torvum) in acetic acid-induced ulcerative colitis in male Wistar rats. Methods: The animals were divided into various treatment groups (n=5). Rats were administered with 2ml of acetic acid (4%) via intrarectal route. Prednisolone was used as a standard drug and S. torvum was administered at a dose of 100 and 300mg/kg, p.o. Macroscopic score, colon weight to length ratio, colonic superoxide dismutase (SOD), reduced glutathione (GSH), myeloperoxidase (MPO), catalase (CAT), and lipid peroxidation (TBARS) levels and histopathological changes were recorded after the treatment regimen of 11 days.
Results: Intrarectal instillation of acetic acid caused significant (P<0.05) increase in colon weight to length ratio, TBARS, and MPO levels. It caused significant (P<0.05) decrease in the levels of SOD, CAT and GSH levels. Pretreatment with S. torvum (100&300mg/kg, p.o.) exhibited significant (p<0.05) increase in the levels of SOD, CAT and reduced GSH and significant decrease in TBARS and MPO levels. Histopathological changes showed that acetic acid treatment caused significant structural damage to colon like inflammation, ulceration with a loss of 40% mucosa which was reversed by S. torvum (100&300mg/kg) treatment.
Conclusion: The present investigation demonstrates the potent therapeutic value of S.torvum (100, 300 mg/kg, p.o.) in the amelioration of experimental ulcerative colitis in rats.



Langholz E, Munkholm P, Davidsen M, Binder V. Course of Ulcerative Colitis: Analysis of Changes in Disease Activity Over Years. Gastroenterology 1994; 107: 3-11. https://doi.org/10.1016/0016-5085(94)90054-X

Strober W, Fuss IJ. Proinflammatory cytokines in the pathogenesis of Inflammatory bowel disease. Gastroenterology 2011; 140(6): 1756-1767. https://doi.org/10.1053/j.gastro.2011.02.016

Baugmart DC, Carding SR. Inflammatory bowel disease: cause and immunobiology. Lancet 2007; 369(9573): 1627-1640. https://doi.org/10.1016/S0140-6736(07)60750-8

Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease. World J Gastroenterol 2007; 13: 5581-5593. https://doi.org/10.3748/wjg.v13.i42.5581

Li XL, Cai YQ, Qin H, Wu YJ. Theurapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis. Can J Physiol Pharmacol 2008; 86: 841-849. https://doi.org/10.1139/Y08-089

Hawkins JV, Emmel EL, Feuer JJ, Nedelman MA, Harvey CJ, Klein HJ, Rozmiarek H, Kennedy AR, Lichtenstein GR, Billings PC. Protease activity in a hapten- induced model of ulcerative colitis in rats. Dig Dis Sci 1997; 42: 1969-1980. https://doi.org/10.1023/A:1018887832465

Patil MVK, Kandhare AD, Bhise SD, Bhale S. A comparative cross sectional descriptive market survey of various brands of levofloxacin. Asian J Pharm Biol Res 2011; 1(2): 174-181.

Patil MVK, Kandhare AD, Bhise SD. Evaluation of patient information leaflets on basis of consumer Psychology and opinion. Int J Phar Pharm Sci Res 2011; 1(2): 87-92. https://doi.org/10.4103/2229-5186.93026

Joshi R, Kumar S, Unnikrishnan M, Mukherjee T. Free radical scavenging reactions of Sulfasalazine, 5-Amino salicylic acid and Sulfapyridine: mechanistic aspects and antioxidant activity. Free Radic Res 2005; 39: 1163-1172. https://doi.org/10.1080/10715760500177880

Rahimi R, Mozaffari S, Abdollahi M. On the use of herbal medicines in management of inflammatory bowel diseases: a systemic review of animal and human studies. Dig Dis Sci 2009; 54: 471-480. https://doi.org/10.1007/s10620-008-0368-x

Simonsma JS, Piluek K. Plant resources of South-East Asia 8 (PROSEA). Indonesia: Bogor, 1994; pp. 412.

Kala CP. Ethnomedicinal botany of the Apatani in the Eastern Himalayan region of Indian. J Ethno and Ethnomed 2005; 1: 1-8. https://doi.org/10.1186/1746-4269-1-1

Yahara S, Yamashita T, Nozawa N, Nohara T. Steroidal glycosides from Solanum torvum. Phytochemistry 1996; 43: 1069-1074. https://doi.org/10.1016/S0031-9422(96)00396-2

Carabot CA, Blunden G, Patel VA. Chlorogenone and neochlorogenone from the unripe fruits of Solanum torvum. Phytochemistry 1991; 30: 1339-1341. https://doi.org/10.1016/S0031-9422(00)95233-6

Mahmood U, Shukla YN, Thakur RS. Non-alkaloidal constituents from Solanum torvum leaves. Phytochemistry 1983; 22: 167-170. https://doi.org/10.1016/S0031-9422(00)80080-1

Mahmood U, Agrawal PK, Thakur RS. Torvonin-A, a spirostane saponin from Solanum torvum leaves. Phytochemistry 1985; 24: 2456-2457. https://doi.org/10.1016/S0031-9422(00)83069-1

Iida Y, Yanai Y, Ono M, Ikeda T, Nohara T. Three unusual 22?- O-23-hydroxy(5?) –spirostanol glycosides from the fruits of Solanum torvum. Chem Pharm Bull 2005; 53: 1122-1125. https://doi.org/10.1248/cpb.53.1122

Arthan D, Svasti J, Kittakoop P, Esen A, Svasti J. Furostanol glycoside 26 –O-? glucosidase from leaves of Solanum torvum. Phytochemistry 2006; 67: 27-33. https://doi.org/10.1016/j.phytochem.2005.09.035

Ajaiyeoba EO. Comparative phytochemical and antimicrobial studies of Solanum macrocarpum and Solanum torvum leaves. Fitoterapia 1999; 70: 184 - 186. https://doi.org/10.1016/S0367-326X(98)00041-0

Chah KF, Muko KN and Oboegbulem SI. Antimicrobial activity of methanolic extract of Solanum torvum fruit. Fitoterapia 2000; 71: 187-189. https://doi.org/10.1016/S0367-326X(99)00139-2

Arthan D, Svasti J, Kittakoop P, Pittayakhachonwut D, Tanticharoen M and Thebtaranonth Y. Anti-viral isoflavonoid sulfate and steroidal glycosides from the fruits of Solanum torvum. Phytochemistry 2002; 59: 459-463. https://doi.org/10.1016/S0031-9422(01)00417-4

Israf DA, Lajis NH, Somchit MN and Sulaiman MR. Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen co-administered with an aqueous Solanum torvum extract. Life Sci 2004; 75: 397-406. https://doi.org/10.1016/j.lfs.2003.10.038

Siva Priya M, Srinivas L. Isolation and purification of novel antioxidant from the water extract of Sundakai (Solanum torvum) seeds. Food Chem 2007; 104: 510-517. https://doi.org/10.1016/j.foodchem.2006.11.060

Ndebia EJ, Kamga R and Nchunga-AnyeNkeh B. Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae). AJTCAM 2007; 4: 240-244. https://doi.org/10.4314/ajtcam.v4i2.31214

Telesphore BN, Catherine BF, Gilbert A, Pierre W, Simplice T, Albert DA, Pierre T, Albert K. Anti-ulcerogenic properties of the aqueous and methanolic extracts from the leaves of Solanum torvum (Solanaceae) in rats. J Ethnopharmacol 2008; 119: 135-140. https://doi.org/10.1016/j.jep.2008.06.008

Mohan M, Jaiswal BS and Kasture S. Effect of Solanum torvum on blood pressure and metabolic alterations in fructose hypertensive rats. J Ethnopharmacol 2009; 126: 86-89. https://doi.org/10.1016/j.jep.2009.08.008

Mohan M,Kamble S, and Kasture S. Protective effect of Solanum torvumon doxorubicin-induced nephrotoxicity in rats. Food and Chem Toxico 2010; 48: 436- 440. https://doi.org/10.1016/j.fct.2009.10.042

Gandhi GR, Ignacimuthu S, Paulraj MG and Sasikumar P. Antihyperglycemic activity and antidiabetic effect of methyl caffeate isolated from Solanum torvum Swartz. fruit in streptozotocin induced diabetic rats. Eur JPharmacol 2011; 30(23): 623-31. https://doi.org/10.1016/j.ejphar.2011.09.159

Jaiswal BS and Mohan M. Effect of Solanum torvum on the contractile response of isolated tissues preparation in fructose fed rat. Int J Pharm Bio Sci 2012; 3(3): 161 - 169.

Narayanan Kannan, Chandrasekharan Guruvayoorappan. Protective effect of Bauhinia tomentosa on acetic acid in-duced ulcerative colitis by regulating antioxidant and inflam-matory mediators. IntImmuno Pharmacol 2013; 16: 57-66. https://doi.org/10.1016/j.intimp.2013.03.008

Mithun Vishwanath K Patil, Amit D Kandhare, Sucheta D Bhise. Effect of aqueous extract of Cucumis sativus Linn. Fruit in ulcerative colitis in laboratory animals. Asian Pac J Trop Biomed 2012; S962-S969. https://doi.org/10.1016/S2221-1691(12)60344-X

Amit D Kandhare, Kiran S Raygude, Pinaki Ghosh, Arvindkumar E Ghule, Tejas P Gosavi, Sachin L Badole, Subhash L Bodhankar. Effect of hydroalcoholic extract of Hibiscus rosasinensisLinn. Leaves in experimental colitis in rats. Asian Pac J Trop Biomed 2012: 337-344. https://doi.org/10.1016/S2221-1691(12)60053-7

Thamotharan G, Sekar G, Ganesh T, Saikat Sen, Raja Chakraborty, Senthil Kumar N. Anti-ulcerogenic effects of Lanta camara Linn. Leaves on in vitro models in rats. Asian J Pharm Clin Res 2010; 3: 57-60.

Vaclavikova R, Kondrova E , Ehrilchova DM, Boumendjel A, Kovar J, Stopka P, et al. The effect of flavonoid derivatives on doxorubicin transport and metabolism. Biorg Med Chem 2008; 16: 2034-2042. https://doi.org/10.1016/j.bmc.2007.10.093

Woisky R, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Agri Res 1998; 37: 99-105. https://doi.org/10.1080/00218839.1998.11100961

Millar AD, Rampton DS, Chander CL, Claxson AWD, Blades S, Coumbe A, Marris CJ, Blake D. Evaluating the antioxidant potential of new treatments for inflammatory bowel disease using rat model of colitis. Gut 1996; 39: 407-415. https://doi.org/10.1136/gut.39.3.407

Hossam MM, Ramadan A, Hemeida, Ali IM, Farid MA. Prophylactic role of Curcumn in dextran sulphate sodium (DSS) induced ulcerative colitis murine model. Food Chem Toxicol 2009; 47: 1311-1317. https://doi.org/10.1016/j.fct.2009.03.003

Saggu H, Cooksey J, Dexter DA. Selective increase in particular superoxide dismutase activity in parkinsonian subtansianigra. J Neurochem 1989; 53: 692-697. https://doi.org/10.1111/j.1471-4159.1989.tb11759.x

Beers JR, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952; 115: 133-140.

Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959; 82: 70-77. https://doi.org/10.1016/0003-9861(59)90090-6

Niehaus WG, Samuelsson B. Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968; 82: 70-77.

Bradley PD, Friebal DA, Christensen RD. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 1982; 78: 206-209. https://doi.org/10.1111/1523-1747.ep12506462

Strober W. Interactions between epithelial cells and immune cells in the intestine. Ann N Y Acad Sci 1998; 859(17): 37-45. https://doi.org/10.1111/j.1749-6632.1998.tb11109.x

Sunkara S, Swanson G, Forsyth CB, Keshavarzian A. Chronic inflammation and malignancy in ulcerative colitis. Ulcers 2011; 1-8. https://doi.org/10.1155/2011/714046

Kruidenier L, Verspaget HW. Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease–radicals or ridiculous? Aliment Pharmacol Ther 2002; 16: 1997-2015. https://doi.org/10.1046/j.1365-2036.2002.01378.x

Kumar KS, Vaishnaw YN, Weiss JF. Radioprotection by anti-oxidant enzyme activity in aspirin-treated rats. Gen Pharmacol 1988; 26(3): 613-617. https://doi.org/10.1016/0306-3623(94)00234-E

Halliwell B, Gutteridge JMC. Free radicals in biology and medicine 2nd ed., Oxford: Clarendon press 1999.

Mahgoub AA, El-Medany AA, Hager HH, Mustafa AA, El-Sabah DM. Evaluating the prophylactic potential of zafirlukast against the toxic effects of acetic acid on the rat colon. Toxicol Lett 2003; 145: 79-87. https://doi.org/10.1016/S0378-4274(03)00269-8

Demir S, Yilmaz M, Koseoglu M, Aydin A. Role of free radicals in peptic ulcer and gastritis. Turk J Gastroenterol 2003; 14: 39?43.

Eiserich JP, Hristova M, Cross CE, Jones AD, Freeman BA, Halliwell B, van derVliet A. Formation of nitric oxide-derived inflammatory oxidants by myeloperoxidase in neutrophils. Nature 1998; 391: 393-397. https://doi.org/10.1038/34923

Coelho RG, Batista LM, Santos LC, Brito ARMS, Vilegas W. Phytochemical study and antiulcerogenic activity of Syngonanthusbisulcatus (Eriocaulaceae) Brazilian. J Pharmaceut Sci 2006; 42(3): 413-417. https://doi.org/10.1590/S1516-93322006000300010

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