The Possibility of Using Allelic Variants of Electrophoretic Spectra of Storage Proteins in the Breeding of Sunflower
PDF

Keywords

Line
Hybrid
Variability
Sunflower
Inheritance
Productivity
Morphological sign
Electrophoretic spectrum of storage protein

How to Cite

Aksyonov, I. (2024). The Possibility of Using Allelic Variants of Electrophoretic Spectra of Storage Proteins in the Breeding of Sunflower. Journal of Basic & Applied Sciences, 20, 143–150. https://doi.org/10.29169/1927-5129.2024.20.15

Abstract

Relevance of the provided research work lies in the need to create parental lines of sunflower with high genetic purity, high combinatorial ability to obtain modern hybrids capable of forming a high level of yield in the arid conditions of the Steppe of Ukraine. Analysis and study of allelic variants of electrophoretic spectra makes it possible to expand the genetic diversity of the source material identified by the spectra of seed storage proteins. The main goal was to establish and study allelic variants of sunflower seed storage proteins, their use in individual plant selection in the process of creating self-pollinated lines, with the subsequent inclusion of lines in the breeding process. The source material for creating self-pollinated lines were sunflower varieties and interspecific hybrids. Plant selection was carried out according to morphological characteristics and allelic variants of the electrophoretic spectra of sunflower seed storage proteins according to the method of F. O. Poperel. The research was carried out in accordance with generally accepted methods in plant breeding. As a result of the conducted studies, allelic variants of electrophoretic spectra of sunflower seed storage proteins Hel 1, Hel 2, Hel 3, Hel 4, Hel 5, Hel 6, Hel 7, Hel 9, Hel NK were established. New self-pollinated sunflower lines with a high level of genetic purity with identified loci Hel 7, Hel 9, Hel NK were created. The creation of starting material from interspecific hybrids ensured the production of genotypes with new helianthine spectra and contributed to the expansion of the genetic diversity of sunflower. Based on allelic variants of protein spectra, protein spectra were developed and recorded for each self-pollinated sample and line created. Self-pollinated lines were created using protein spectra and clustering of the resulting genotypes was performed. Sunflower hybrids with the participation of lines from the most genetically distant clusters form a yield higher by 0.5-0.8 t ha-1. The established allelic variants of the electrophoretic spectra of seed storage proteins are recommended to be used in individual plant selection in the process of obtaining self-pollinated sunflower lines with a high level of genetic purity. The obtained sunflower lines are recommended to be used in the creation of hybrids intended for cultivation in arid steppe conditions of Ukraine.

https://doi.org/10.29169/1927-5129.2024.20.15
PDF

References

Surovikin VN, Borodin SG. About the breeding of sunflower varieties to reduce the growing season. Breeding and seeds production 1991; 4: 12-15.

Volgin VV, Obydalo AD, Bochkaryov BN. Double-tester estimation of inheritance of plant height in interline sunflower hybrids. Oilseed Crops 2019; 4(180): 3-9. https://doi.org/10.25230/2412-608X-2019-4-180-3-9

Kovacik A, Skaloud V. Collection of sunflower marker genes available for genetic studies. Helia 1980; 3: 27-28.

Bochkaryov NI. On the ideotype of self-pollinated sunflower lines. Scientific and Technical Bulletin of VNIIMK 1995; 116: 16-20.

Stancovic V. Phenotypic and correlations of morphological traits and yield components of protein sunflower (Helianthus annuus L.). M.Sc. Thesis. University of Novi Sad 2005; pp. 1-68.

Suvorova YuN. Estimation of valuable traits of the Siberian sunflower samples in the southern forest-steppe of the Western Siberia. Oilseed Crops 2019; 1(177): 10-16. https://doi.org/10.25230/2412-608X-2019-1-177-10-16

Lobachyov YuV, Kurasova LG, Lekarev VM, Konstantinova EA. Genetic control of the shape of ray flowers in almost isogenic lines of sunflower. Scientific and Technical Bulletin of VNIIMK 2010; 2(144-145): 21-25.

Kostevich SV, Medvedeva NV. Development of the new maternal lines of sunflower with short tubular ray flowers as a marker trait. Scientific and Technical Bulletin of the All-Russian Research Institute of Oilseeds Crops 2018; 4(176): 9-15. https://doi.org/10.25230/2412-608X-2018-4-176-9-15

Aksyonov IV, Mishchenko LYu. Use of electrophoretic spectra of reserve seed proteins to improve typicalness of sunflower parental lines. Russian Agricultural Sciences 2016; 42(3): 211-214. https://doi.org/10.3103/S1068367416030034

Konarev AV. The use of molecular markers in solving the problem of plant genetic resources and breeding. Agrarian Russia 2006; 6: 4-21.

Guchetl SZ, Zaitsev NI, Frolov SS, Frolova IN, Kuznetsova ES. Genotyping of sunflower inbred lines and hybrids bred at the Armavirskaay experimental station using SSR-loci. Oilseed Crops 2019; 3(179): 27-31. https://doi.org/10.25230/2412-608X-2019-3-179-27-34

Sozinov AA. Protein polymorphism and its importance in genetics and breeding. Moscow: Nauka 1985.

Konarev AV. Adaptive nature of molecular polymorphism and its use in solving problems of plant genetic resources and breeding. Agrarian Russia 2002; 36: 1-16.

Allard RW. Genetic basic of the evolution of the adaptedness in plants. Adaptation in Plant Breeding 1997; 1-11. https://doi.org/10.1007/978-94-015-8806-5_1

Anisimova IN. Heliantinin polymorphism and its use in the identification of sunflower varieties, lines and hybrids. Agricultural Biology 1991; 1: 88-95.

Solodenko A, Sivolap Y. Genotyping of Helianthus based on microsatellite sequences. Helia 2006; 28(42): 19-26. https://doi.org/10.2298/HEL0542019S

Gavrilova VA, Anisimova IN. Genetics of cultivated plants. Sunflower. Sankt-Petersburg: VIR 2003.

Konarev AV, Konarev VG, Gubareva NK, Peneva VG. Seed proteins as markers in solving the problems of plant genetic resources, breeding and seed production. Cytology and Genetics 2000; 34(2): 91-104.

Aksyonov I. Use of protein spectra in increasing the genetic homogenous traits of the parental lines of sunflower. Helia 2014; 37(60): 87-98. https://doi.org/10.1515/helia-2014-0007

Gubareva NK, Martinenko NM, Zuev EV, Brikova AN. Prospects for the use of electrophoresis of grain proteins to control genetic integrity, refine passport data and identify doublets in the collection of spring soft wheat. Works on Applied Botany, Genetics and Selection 2002; 120: 158-165.

Lakin GF. Biometrics. Moscow: Higher School 1990.

Myatlev VD, Panchenko VD, Reznicenko LA, Terekhin AT. Probability Theory and Mathematical Statistics. Mathematical Models. Moscow: Publishing Center "Academy" 2009.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.