Influence of Parmigiano Reggiano Diet on Male Sexual Behavior in Rats: Behavioral and Neurochemical Study


 Parmigiano Reggiano, male rat, sexual behavior, cerebral microdialysis, dopamine.  

How to Cite

Di Viesti, G. Carnevale, M.M. Carrozzo, M. Zavatti, & M. Baraldi. (2012). Influence of Parmigiano Reggiano Diet on Male Sexual Behavior in Rats: Behavioral and Neurochemical Study . Journal of Pharmacy and Nutrition Sciences, 2(1), 77–82.


The influence of Parmigiano Reggiano (P.R.) cheese on copulatory behavior was studied in male rats. Sexually naїve, sluggish and potent rats were chronically fed with a diet of P.R. cheese for 10 d. Mount, intromission, ejaculation latencies and the percentage of mounting and ejaculating animals were recorded during the mating test. Microdialysis technique was used to detect the extracellular levels of dopamine (DA) and its metabolite dihydroxyphenylacetic acid (DOPAC) in rat brain following the P.R. diet. The P.R. diet was able to improve sexual behavior in naїve rats increasing the percentage of mounting and ejaculating animals. Moreover it was able to reduce latencies of mounts, intromissions and ejaculations and to increase the percentage of mounting and ejaculating animals in sluggish rats. Finally, in the microdialysis study an increase in DA and its metabolite DOPAC was found in P.R. fed naїve rats in comparison with control group.


Coppola R, Nanni M, Iorizzo M, C, et al. Microbiological characteristics of Parmigiano Reggiano cheese during the

cheese making and the first months of the ripening. Lait 2000; 80: 479-90.

Bottari B, Santarelli M, Neviani E, Gatti M. Natural whey starter for Parmigiano Reggiano: culture-independent approach. J Appl Microbiol 2010; 108: 1676-84.

Shintu L, Ziarelli F, Caldarelli S. Is high-resolution magic angle spinning NMR a practical speciation tool for cheese samples? Parmigiano Reggiano as a case study. Mag Reson Chem 2004; 42: 396-401.

Peeters M, Giuliano. Central neurophysiology and dopaminergic control of ejaculation. Neurosci Biobehav Rev 2008; 32: 438-53.

Melis MR, Argiolas A. Dopamine and sexual behavior. Neurosci Biobehav Rev 1995; 19: 19-38.

Blackburn JR, Pfaus JG, Phillips AG. Dopamine functions in appetitive and defensive behaviours. Prog Neurobiol 1992; 39: 247-79.

Dominguez JM, Hull EM. Dopamine, the medial preoptic area, and male sexual behavior. Physiol Behav 2005; 86: 356-68.

Baraldi M, Benassi-Benelli, A. Dissociation of the capacity of apomorphine to evoke penile erection and stereotypy folloeing intragastric administration to adult rats. Farmacol Terap 1975; 361.

Pleim ET, Matochik JA, Barfield RJ, Auerbach SB. Correlation of dopamine release in the nucleus accumbens with masculine sexual behavior in rats. Brain Res 1990; 524: 160-63.

Robinson DL, Philips EMP, Budygin EA, Trafton BJ, Garris PA, Wightman RM. Sub-second changes in accumbal dopamine during sexual behavior in male rats. Neuro Report 2001; 12: 2549-52.

Damsma G, Pfaus JG, Wenkstern DG, Phillips AG, Fibiger HC. Sexual behavior increases dopamine transmission in the nucleus accumbens and striatum of male rats: Comparison with novelty and locomotion. Behav Neurosci 1992; 106: 181-91.

Ågmo A. Male rat sexual behaviour. Brain Res Brain Res Protoc 1997; 1: 203-209.

Zanoli P, Benelli A, Rivasi M, Baraldi C, Vezzalini F, Baraldi M. Opposite effect of acute and subchronic treatments with Ferula hermonis on copulatory behavior of male rats. Int J Imp Res 2003; 15: 450-55.

Zanoli P, Benelli A, Zavatti M, Rivasi M, Baraldi C, Baraldi M. Improved sexual behavior in male rats treated with a Chinese herbal extract: hormonal and neuronal implications. Asian J Androl 2008; 10: 937-45.

Dewsbury DA. Effect of tetrabenazine on the copulatory behavior in male rats. Eur J Pharmacol 1972; 17: 221-26.

Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. 2nd edn, Academic Press: New York 1986.

Bitran D, Hull EM. Pharmacological analysis of male rat sexual behavior. Neurosci Biobehav Rev 1987; 11: 365-89.

Hull EM, Du J, Lorrain DS, Matuszewich L. Extracellular dopamine in the medial preoptic area: implications for sexual motivation and hormonal control of copulation. J Neurosci 1995; 15: 7465-71.

Sato Y, Wada H, Horita H, et al. Dopamine release in the medial preoptic area during male copulatory behavior in rats. Brain Res 1995; 692: 66-70.

Triemstra JL, Nagatani S, Wood RI. Chemosensory cues are essential for mating-induced dopamine release in MPOA of male Syrian hamsters. Neuropsychopharmacology 2005; 30: 1436-42.

Hull EM, Meisel RL, Sachs BD. Male sexual behavior. In: Pfaff DW, Arnold AP, Etgen AM, Fahrbach SE, Rubin RT, editors. Hormones brain and behavior. San Diego, CA: Academic Press 2002; pp. 3-127.

Melis MR, Succu S, Mascia MS, Cortis L, Argiolas A. Extra-cellular dopamine increases in the paraventricular nucleus of male rats during sexual activity. Eur J Neurosci 2003; 17: 1266-72.

Everitt BJ. Sexual motivation: a neural and behavioural analysis of the mechanisms underlying appetitive and copulatory responses of male rats. Neurosci Biobehav Rev 1990; 14: 217-32.

Mas M, Fumero B, Gonzalez-Mora JL. Voltammetric and microdialysis monitoring of brain monoamine neurotransmitter release during sociosexual interactions. Behav Brain Res 1995; 71: 69-79.

Fiorino DF, Coury A, Phillips AG. Dynamic changes in nucleus accumbens dopamine efflux during the Coolidge effect in male rats. J Neurosci 1997; 17: 4849-55.

Baraldi M, Benassi-Benelli A, Bernabei MT, Cameroni R, Ferrari F, Ferrari P. Apocodeine induced stereotypes and penile erections in rats. Neuropharmacology 1979; 18: 165.

Pfaus JG, Phillips AG. Differential effects of dopamine receptor antagonists on the sexual behavior of male rats. Psychopharmacology 1989; 98: 363-368.

Giuliani D, Ottani A, Ferrari F. Influence of sildenafil on copulatory behaviour in sluggish or normal ejaculator male rats: a central dopamine mediated effect? Neuropharmacology 2002; 42: 562-67.

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