This study aimed to examine effects of quercetin on 1) endurance capacity 2) malondialdehyde (MDA) and superoxide dismutase (SOD) activity in skeletal muscle and 3) muscle fibre density and size in mice after an intense exercise. There were 5 groups: control, vitamin C (250 mg/kg body weight), quercetin 150, 300, and 450 mg/kg body weight respectively once a day for 28 days. Endurance capacity was measured by exhaustive swimming exercise test which was done 24-h after swimming at high intensity. Then muscles were analyzed for MDA, SOD activity, and muscle fibre density and size. After the 28-day treatment, endurance time in vitamin C and quercetin treated groups at dose of 150 mg/kg body weight were longer than the vehicle group (p<0.05). MDA concentration in extensor digitorum longus muscle was lower than vehicle group in vitamin C, Q150 and Q450 groups and in soleus muscle the highest was in Q300 group. SOD activity in soleus muscle in Q150 group was higher than vitamin C and Q450 groups. Significant increase in cell diameter of soleus muscle was found (p<0.05). Current findings suggest that ingestion of quercetin can improve endurance capacity, due probably to increased antioxidant activity and size of muscle fibre type 1.
Davison GW, Ashton T, McEneny J, Young IS, Davies B, Bailey DM. Critical difference applied to exercise-induced oxidative stress: the dilemma of distinguishing biological from statistical change. J Physiol Biochem 2012; [Epub ahead of print
Kuwahara H, Horie T, Ishikawa S, et al. Oxidative stress in skeletal muscle causes severe disturbance of exercise activity without muscle atrophy. Free Radic Biol Med 2010; 48: 1252-62. http://dx.doi.org/10.1016/j.freeradbiomed.2010.02.011
Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 2008; 88: 1243-76. http://dx.doi.org/10.1152/physrev.00031.2007
Goldfarb AH, Bloomer RJ, McKenzie MJ. Combined antioxidant treatment effects on blood oxidative stress after eccentric exercise. Med Sci Sports Exerc 2005; 37: 234-9. http://dx.doi.org/10.1249/01.MSS.0000152887.87785.BE
Cureton KJ, Tomporowski PD, Singhal A, et al. Dietary quercetin supplementation is not ergogenic in untrained men. J Appl Physiol 2009; 107: 1095-104. http://dx.doi.org/10.1152/japplphysiol.00234.2009
Bischoff SC. Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care 2008; 11: 733-40. http://dx.doi.org/10.1097/MCO.0b013e32831394b8
Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol 2008; 585: 325-37. http://dx.doi.org/10.1016/j.ejphar.2008.03.008
Cai Q, Rahn RO, Zhang R. Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Lett 1997; 119: 99-107. http://dx.doi.org/10.1016/S0304-3835(97)00261-9
Choi JA, Kim JY, Lee JY, et al. Induction of cell cycle arrest and apoptosis in human breast cancer cells by quercetin. Int J Oncol 2001; 19: 837-44.
Davis JM, Murphy EA, Carmichael MD, Davis B. Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am J Physiol Regul Integr Comp Physiol 2009; 296: R1071-7. http://dx.doi.org/10.1152/ajpregu.90925.2008
Davis JM, Carlstedt CJ, Chen S, Carmichael MD, Murphy EA. The dietary flavonoid quercetin increases VO(2max) and endurance capacity. Int J Sport Nutr Exerc Metab 2010; 20: 56-62.
Kressler J, Millard-Stafford M, Warren GL. Quercetin and endurance exercise capacity: a systematic review and meta-analysis. Med Sci Sports Exerc 2011; 43: 2396-404. http://dx.doi.org/10.1249/MSS.0b013e31822495a7
MacRae HSH, Mefferd KM. Dietary antioxidant supplementation combined with quercetin improves cycling time trial performance. Int J Sport Nutr Exerc Metab 2006; 16(4): 405-19.
Nieman DC, Williams AS, Shanely RA, et al. Quercetin’s influence on exercise performance and muscle mitochondrial biogenesis. Med Sci Sports Exerc 2010; 42: 338-45. http://dx.doi.org/10.1249/MSS.0b013e3181b18fa3
Bigelman KA, Fan EH, Chapman DP, Freese EC, Trilk JL, Cureton KJ. Effects of six weeks of quercetin supplementation on physical performance in ROTC cadets. Mil Med 2010; 175: 791-8.
Ganio MS, Armstrong LE, Johnson EC, et al. Effect of quercetin supplementation on maximal oxygen uptake in men and women. J Sports Sci 2010; 28: 201-8. http://dx.doi.org/10.1080/02640410903428558
Quindry JC, McAnulty SR, Hudson MB, et al. Oral quercetin supplementation and blood oxidative capacity in response to ultramarathon competition. Int J Sport Nutr Exerc Metab 2008; 18: 601-16.
Utter AC, Nieman DC, Kang J, et al. Quercetin does not affect rating of perceived exertion in athletes during the Western States endurance run. Res Sports Med 2009; 17: 71-83. http://dx.doi.org/10.1080/15438620902901474
Abe T, Takiguchi Y, Tamura M, Shimura J, Yamazaki K. Effect of vespa amino acid mixture (VAAM) isolated from hornet larval saliva and modified VMM nutrients on endurance exercise in swimming mice improvement in performance and changes of blood lactate and glucose. Jpn J Physiol Fitness Sports Med 1995; 44: 225-38.
Ikeuchi M, Koyama T, Takahashi J, Yazawa K. Effects of astaxanthin supplementation on exercise-induced fatigue in mice. Biol Pharm Bull 2006; 29: 2106-10. http://dx.doi.org/10.1248/bpb.29.2106
Marzel, P. General principle and procedure for drug metabolism in vitro. In: La Du BN, Mandel HG, Way EL editors. Fundamentals of drug metabolism and drug disposition. New York: Krieger Publishing Company 1979; pp. 527-52.
Liu J, Edamatsu R, Kabuto H, Mori A. Antioxidant action of guilingji in the brain of rats with FeCl3-induced epilepsy. Free Radic Biol Med 1990; 9: 451-4. http://dx.doi.org/10.1016/0891-5849(90)90023-C
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
Yu FR, Liu Y, Cui YZ, et al. Effects of a flavonoid extract from Cynomorium songaricum on the swimming endurance of rats. Am J Chin Med 2010; 38: 65-73. http://dx.doi.org/10.1142/S0192415X10007774
Cheuvront SN, Ely BR, Kenefick RW, Michniak-Kohn BB, Rood JC, Sawka MN. No effect of nutritional adenosine receptor antagonists on exercise performance in the heat. Am J Physiol Regul Integr Comp Physiol 2009; 296: R394-401. http://dx.doi.org/10.1152/ajpregu.90812.2008
Dumke CL, Nieman DC, Utter AC, et al. Quercetin’s effect on cycling efficiency and substrate utilization. Appl Physiol Nutr Metab 2009; 34: 993-1000. http://dx.doi.org/10.1139/H09-099
Freese E, Chapman D, Ahsan S. Dietary Quercetin supplementation does not increase maximal oxygen uptake and physical work capacity. Annual Meeting of the SEACSM 2010; 38: O1611-3.
Manach C, Williamson G, Morand C, Scalbert A, Rémésy C. “Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies1–3” Am J Clin Nutr 2005; 81(suppl): 230S-42S.
Geng T, Li P, Okutsu M, et al. PGC-1alpha plays a functional role in exercise-induced mitochondrial biogenesis and angiogenesis but not fibre-type transformation in mouse skeletal muscle. Am J Physiol Cell Physiol 2010; 298: C572-9. http://dx.doi.org/10.1152/ajpcell.00481.2009
Lin J, Wu H, Tarr PT, et al. Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature 2002; 418: 797-801. http://dx.doi.org/10.1038/nature00904
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2012 N. Leelayuwat; S. Laddawan, Y. Kanpetta, M. Benja, D. Wongpan, O. Tunkamnerdthai, J. Wattanathorn, S. Muchimapura , J. Yamauchi