Enrichment of Bio-Active Phthalides in Celery Seed Oil
DOI:
https://doi.org/10.6000/1927-5951.2013.03.04.6Keywords:
Celery seed oil, enrichment, fractional distillation, phthalides, GC-MS.Abstract
Objective: To develop an efficient process to obtain a phthalide-enriched nutraceutical fraction from celery seed oil.
Methods: Three approaches viz., fractional distillation, column chromatography and solvent-solvent partition, have been used.
Results: Fractional distillation of celery seed oil (13.7g) afforded i) a limonene-rich fraction (7.6g, 97% purity), ii) a fraction containing β-selinene (2.8 g, 90 % purity) and iii) one containing phthalides (2.9 g, 90 % purity). Solvent-solvent partition of celery seed oil gave limonene (87%) and a fraction containing phthalides (49%), which on further fractionation afforded a phthalide-enriched fraction (90%). By conventional silica gel column chromatography, a product rich in phthalides (53-74%) could be obtained.
Conclusion: Fractional distillation is shown to be a viable method to process celery seed oil into a phthalide-enriched product with high nutraceutical potential.
References
Bjeldanes LF, Kim I. Phthalide components of celery essential oil. J Org Chem 1977; 42: 2333-35. http://dx.doi.org/10.1021/jo00433a033
Sowbhagya HB, Srinivas P, Krishnamurthy N. Effect of enzymes on extraction of volatiles from celery seeds. J Food Chem 2010; 120: 230-34. http://dx.doi.org/10.1016/j.foodchem.2009.10.013
Wilson CW. Relative recovery and identification of carbonyl compounds from celery essential oil. J Food Sci 1970; 35: 766-68. http://dx.doi.org/10.1111/j.1365-2621.1970.tb01989.x
Wilson CW III. Identification and quantitative estimation of alcohols in celery essential oil. J Food Sci 1969; 34: 535-37. http://dx.doi.org/10.1111/j.1365-2621.1969.tb12081.x
Bradley PR. British herbal compendium, Volume 1. BHMA, Bournemouth 1992.
Momin RA, Nair MG. Antioxidant, cyclo-oxygenase and topoisomerase inhibitory compounds from Apium graveolens Linn. Seeds. Phytomedicine 2002; 9: 312-18. http://dx.doi.org/10.1078/0944-7113-00131
Zheng GQ, Kenney PM, Zhang J, Lam LKT. Chemoprevention of benzo[a
Butters DE, Davis CKC, McGeary RP, Powanda MC, Rainsford KD, Whitehouse MW. Extracts of celery seed for the prevention and treatment of pain, inflammation and gastrointestinal irritation 2003; US Patent 6, 576, 274.
Rose R, Chrisope GL. Product and method for treating joint disorders in vertebrates. 1999; US Patent 5, 916, 565.
Gold HJ, Wilson CW III. The volatile flavour substances of celery. J Food Sci 1963; 28: 484-88. http://dx.doi.org/10.1111/j.1365-2621.1963.tb00231.x
Uhlig JW, Chang A, Jen JJ. Effect of phthalides on celery flavour. J Food Sci 1987; 52: 658-60. http://dx.doi.org/10.1111/j.1365-2621.1987.tb06696.x
Sowbhagya HB, Sampathu SR, Krishnamurthy N. Evaluation of size reduction on the yield and quality of celery seed oil. J Food Eng 2007; 80: 1255-60. http://dx.doi.org/10.1016/j.jfoodeng.2006.09.019
Sowbhagya HB, Sampahtu SR, Srinivas P. A process for the preparation of phthalides from celery seed oil Indian Patent IN 243756 2010.
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Halagur B. Sowbhagya , Pullabhatla Srinivas
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Denis Kiragu, Raymond L. Tyohemba, Ogbene G. Igbum, Michael Nase, GC/MS Profiling and Antibacterial Activity of Steam-Distilled Solanecio mannii Essential Oil and Hydrosol Against Multidrug-Resistant Food Pathogens , Journal of Pharmacy and Nutrition Sciences : Vol. 15 (2025)
- Nwogo Ajuka Obasi, Chinyere Aloke, Stella Eberechukwu Obasi, Ademola Clement Famurewa, Sunday Oge Elom, Patrck Maduabuchi Aja, Lawrence Olusegun Ajala, Nutritional Assessment of Pulps and Partial Characterization of Seed Oils from Varieties of Pear Fruits , Journal of Pharmacy and Nutrition Sciences : Vol. 13 (2023)
- Federico Ranalli, Alfonso Ranalli, Stefania Contento, Maria Casanovas, Mariangela Antonucci , Guido Di Simone, Concentrations of Bioactives and Functional Factors in Destoned Virgin Olive Oil: The Case Study of the Oil from Olivastra di Seggiano Cultivar , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 1 (2012)
- Fahima Aktar, Md. Ruhul, Sk. Omar Faruque, Farhana Rumi, Mohiuddin Abdul, Mohammad A. Rashid, Antioxidant, Cytotoxic, Membrane Stabilizing and Antimicrobial Activities of Bark and Seed of Entada phaseoloides (L.) Merr.: A Medicinal Plant from Chittagong Hill Tracts , Journal of Pharmacy and Nutrition Sciences : Vol. 1 No. 2 (2011)
- Sousan Kolahi, Amir Ghorbanihaghjo, Ali-Reza Khabbazi, Mehrzad Hajialilo , Hale Farzin, The Effect of Fish Oil Supplement on Serum Antioxidant Level in Patient with Rheumatoid Arthritis , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 4 (2013)
- Rashidi Othman , Razanah Ramya , Norazian Mohd. Hassan , Suhair Kamoona, Qualitative and Quantitative Phenolic Compounds Analysis of Dicranopteris linearis Different Fractional Polarities Leaves Extract , Journal of Pharmacy and Nutrition Sciences : Vol. 10 No. 1 (2020)
- Md. Saddam Hussain, Mohammad Salim Hossain , Md. Mamun Or Rashid, Antiobesity and Lipid Lowering Activitiy of Vigna unguiculata (L) Walp. Seed in High Fat Diet Induced Obese Mice , Journal of Pharmacy and Nutrition Sciences : Vol. 9 No. 4 (2019)
- Zahra Pilevar , Hedayat Hosseini, Chemical Composition, Antimicrobial and Antioxidant Activity of Echinophora platyloba DC , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 4 (2013)
- Mohammed Seid, Aman Dekebo, Neelaiah Babu, Phytochemical Investigation and Antimicrobial Evaluation of Foeniculum vulgare Leaves Extract Ingredient of Ethiopian Local Liquor , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 1 (2018)
- Robinson, B. Lu, I. Edirisinghe , C.T. Kappagoda, Effect of Grape Seed Extract on Blood Pressure in Subjects with Pre-Hypertension , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 2 (2012)
You may also start an advanced similarity search for this article.