Enrichment of Bio-Active Phthalides in Celery Seed Oil


  • Halagur B. Sowbhagya CSIR-Central Food Technological Research Institute, Mysore-570020, India
  • Pullabhatla Srinivas CSIR-Central Food Technological Research Institute, Mysore-570020, India




 Celery seed oil, enrichment, fractional distillation, phthalides, GC-MS.


Objective: To develop an efficient process to obtain a phthalide-enriched nutraceutical fraction from celery seed oil.
Methods: Three approaches viz., fractional distillation, column chromatography and solvent-solvent partition, have been used.
Results: Fractional distillation of celery seed oil (13.7g) afforded i) a limonene-rich fraction (7.6g, 97% purity), ii) a fraction containing β-selinene (2.8 g, 90 % purity) and iii) one containing phthalides (2.9 g, 90 % purity). Solvent-solvent partition of celery seed oil gave limonene (87%) and a fraction containing phthalides (49%), which on further fractionation afforded a phthalide-enriched fraction (90%). By conventional silica gel column chromatography, a product rich in phthalides (53-74%) could be obtained.
Conclusion: Fractional distillation is shown to be a viable method to process celery seed oil into a phthalide-enriched product with high nutraceutical potential.


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How to Cite

Halagur B. Sowbhagya, & Pullabhatla Srinivas. (2013). Enrichment of Bio-Active Phthalides in Celery Seed Oil. Journal of Pharmacy and Nutrition Sciences, 3(4), 250–257. https://doi.org/10.6000/1927-5951.2013.03.04.6