Effects of Long-Term Administration of Freeze-Dried Chokeberry Juice to Rats

Authors

  • Teresa Adamska Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland
  • Małgorzata Ewertowska Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland
  • Ewa Ignatowicz Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland
  • Małgorzata Kujawska Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland
  • Marcin Kidoń Pozna University of Life Sciences, 31 Wojska Polskiego Str., 60-624 Pozna, Poland
  • Arleta Matuszewska Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland
  • Jan Oszmiański Environmental and Life Science University, 37/41 Che mo skiego Str., 51-630 Wroc aw, Poland
  • Hanna Szaefer Pozna University of Medical Sciences, 4 wi cickiego Str., 60-761 Pozna , Poland
  • Jadwiga Jodynis-Liebert Pozna University of Medical Sciences, 30 Dojazd Str., 60-631 Pozna , Poland

DOI:

https://doi.org/10.6000/1927-5951.2014.04.02.11

Keywords:

antioxidant activity, hematology, clinical chemistry, Chokeberry

Abstract

Polyphenolics can act as prooxidants leading to the generation of reactive oxygen species and electrophilic metabolites which bind to DNA, protein and glutathione. The aim of the present study was to evaluate potential adverse effects of the long-term dietary administration of freeze-dried chokeberry juice to rats.
Groups of 8 males and 8 females were exposed via diet to 0; 2; 6; 10 g juice/kg feed for 90 days. Mean food consumption and mean body weight gain of treated animals were comparable with controls. Changes in some hematological parameters were sporadic and non-dose-responsive. Several statistically significant changes in clinical chemistry parameters were considered no toxicologically relevant since they were of small magnitude and lacked correlating findings in histopathology. Histopathological examination did not reveal any changes that could be attributed to chokeberry juice intake. Determination of oxidative damage markers in the liver demonstrated no damage of lipids, proteins and DNA. Chokeberry juice intake improved antioxidant status of rats as evidenced by a decrease in the level of lipid peroxidation, an increase in reduced glutathione concentration and an increase in some antioxidant enzymes activity.
It could be concluded that freeze-dried chokeberry juice is safe at doses tested and can be used as a component of food supplements.

References

Dragsted LO, Pedersen A, Hermetter A, et al. The 6-a-day study: effects of fruit and vegetables on markers of oxidative stress and antioxidative defense in healthy nonsmokers. Am J Clin Nutr 2004; 79: 1060-72.

Espín JC, García-Conesa MT, Tomás-Barberán FA. Nutraceuticals: facts and fiction. Phytochemistry 2007; 68: 2986-3008. http://dx.doi.org/10.1016/j.phytochem.2007.09.014

Pool-Zobel BL, Bub A, Schröder N, Rechkemmer G. Anthocyanins are potent antioxidants in model systems but do not reduce endogenous oxidative DNA damage in human colon cells. Eur J Nutr 1999; 38: 227-34. http://dx.doi.org/10.1007/s003940050065

Gasiorowski K, Szyba K, Brokos B, Ko?aczy?ska B, Jankowiak-W?odarczyk M, Oszmia?ski J. Antimutagenic activity of anthocyanins isolated from Aronia melanocarpa fruits. Cancer Lett 1997; 119: 37-46. http://dx.doi.org/10.1016/S0304-3835(97)00248-6

Jing P, Bomser JA, Schwartz SJ, He J, Magnuson BA, Giusti MM. Structure-function relationships of anthocyanins from various anthocyanin-rich extracts on the inhibition of colon cancer cell growth. J Agric Food Chem 2008; 56: 9391-98. http://dx.doi.org/10.1021/jf8005917

Lala G, Malia M, Zhao C, et al. Anthocyanin-rich extracts inhibit multiple biomarkers of colon cancer in rats. Nutr Cancer 2006; 54: 84-93. http://dx.doi.org/10.1207/s15327914nc5401_10

Valcheva-Kuzmanova S, Kuzmanov K, Mihova V, Krasnaliev I, Borisova P, Belcheva A. Antihyperlipidemic effect of Aronia melanocarpa fruit juice in rats fed a high-cholesterol diet. Plant Foods Hum Nutr 2007; 62: 19-24. http://dx.doi.org/10.1007/s11130-006-0036-2

Kokotkiewicz A, Jaremicz Z, Luczkiewicz M. Aronia plants: a review of traditional use, biological activities, and perspectives for modern medicine. J Med Food 2010; 13: 255-69. http://dx.doi.org/10.1089/jmf.2009.0062

Valcheva-Kuzmanova S, Borisova P, Galunska B, Krasnaliev I, Belcheva A. Hepatoprotective effect of the natural fruit juice from Aronia melanocarpa on carbon tetrachloride-induced acute liver damage in rats. Exp Toxicol Pathol 2004; 56: 195-201. http://dx.doi.org/10.1016/j.etp.2004.04.012

Kujawska M, Ignatowicz E, Ewertowska M, Oszmia?ski J, Jodynis-Liebert J. Protective effect of chokeberry on chemical-induced oxidative stress in rat. Hum Exp Toxicol 2011; 30: 199-208. http://dx.doi.org/10.1177/0960327110371697

Matsumoto M, Hara H, Chiji H and Kasai T. Gastroprotective effect of red pigments in black chokeberry fruit (Aronia melanocarpa Elliot) on acute gastric hemorrhagic lesions in rats. J Agric Food Chem 2004; 52: 2226-29. http://dx.doi.org/10.1021/jf034818q

Galati G, O'Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med 2004; 3: 287-303. http://dx.doi.org/10.1016/j.freeradbiomed.2004.04.034

Awad HM, Boersma MG, Boeren S, van Bladeren PJ, Vervoort J, Rietjens IM. Structure-activity study on the quinone/quinone methide chemistry of flavonoids. Chem Res Toxicol 2001; 14: 398-408. http://dx.doi.org/10.1021/tx000216e

Halliwell B. Dietary polyphenols: good, bad, or indifferent for your health? Cardiovasc Res 2007; 73: 341-47. http://dx.doi.org/10.1016/j.cardiores.2006.10.004

Scalbert A, Manach C, Morand C, Rémésy C, Jiménez L. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr 2005; 45: 287-306. http://dx.doi.org/10.1080/1040869059096

Oszmia?ski J, Wojdy?o A. Aronia melanocarpa phenolics and their antioxidant activity. Eur Food Res Technol 2005; 221: 809-13. http://dx.doi.org/10.1007/s00217-005-0002-5

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26: 1231-37. http://dx.doi.org/10.1016/S0891-5849(98)00315-3

Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV-visible spectroscopy. In: Handbook of Food Analytical Chemistry. John Wiley and Sons, New York 2005; pp. 19-31. http://dx.doi.org/10.1002/0471142913.faf0102s00

Sanz MJ, Ferrandiz ML, Cejudo M, et al. Influence of a series of natural flavonoids on free radical generating systems and oxidative stress. Xenobiotica 1994; 24: 689-99. http://dx.doi.org/10.3109/00498259409043270

Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25: 192-205. http://dx.doi.org/10.1016/0003-2697(68)90092-4

Hartmann A, Agurel E, Beevers EIC, et al. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 2003; 18: 45-51. http://dx.doi.org/10.1093/mutage/18.1.45

Jodynis-Liebert J, Murias M, B?oszyk E. Effect of sesquiterpene lactones on antioxidant enzymes and some drug-metabolizing enzymes in rat liver and kidney. Planta Med 2000; 66: 199-205. http://dx.doi.org/10.1055/s-2000-8566

Mohandas J, Marshall JJ, Duggin GG, Horvath JS, Tiller DJ. Low activities of glutathione-related enzymes as factors in the genesis of urinary bladder cancer. Cancer Res 1984; 44: 5086-91.

Breinholt V, Lauridsen ST, Dragsted LO. Differential effects of dietary flavonoids on drug metabolizing and antioxidant enzymes in female rat. Xenobiotica 1999; 12: 1227-40. http://dx.doi.org/10.1080/004982599237903

Galvez J, de la Cruz JP, Zarzuelo A, Sanchez de la Cuesta F. Flavonoid inhibition of enzymic and nonenzymic lipid peroxidation in rat liver differs from its influence on the glutathione-related enzymes. Pharmacology 1995; 51: 127-33. http://dx.doi.org/10.1159/000139325

Gonzalez-Correa JA, de la Cruz JP, Gordillo J, Urena I, Redondo R, Sanchez de la Cuesta F. Effects of silymarin MZ-80 on hepatic oxidation stress in rats with biliary obstruction. Pharmacology 2002; 64: 18-2. http://dx.doi.org/10.1159/000056146

Nelson SK, Bose SK, Grunwald GK, Myhill P, McCord JM. The induction of human superoxide dismutase and catalase in vivo: a fundamentally new approach to antioxidant therapy. Free Radic Biol Med 2006; 40: 341-7. http://dx.doi.org/10.1016/j.freeradbiomed.2005.08.043

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Published

2014-05-05

How to Cite

Adamska, T., Ewertowska, . M., Ignatowicz, . E., Kujawska, . M., Kidoń, . M., Arleta Matuszewska, Oszmiański, . J., Szaefer, . H., & Jodynis-Liebert, . J. (2014). Effects of Long-Term Administration of Freeze-Dried Chokeberry Juice to Rats. Journal of Pharmacy and Nutrition Sciences, 4(2), 154–161. https://doi.org/10.6000/1927-5951.2014.04.02.11

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