In Vitro Cytotoxic Activity of Isostichopus badionotus, a Sea Cucumber from Yucatan Peninsula Coast


 Holothurian, organic extracts, selectivity index, HeLa cells.

How to Cite

Aida R. Pérez-Espadas, María J. Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cárdenas, María E. Morales-Rubio, Lorena V. León-Deniz, Jaqueline Canul-Canché, & Leovigildo Quijano. (2014). In Vitro Cytotoxic Activity of Isostichopus badionotus, a Sea Cucumber from Yucatan Peninsula Coast . Journal of Pharmacy and Nutrition Sciences, 4(3), 183–186.


The in vitro cytotoxic activity of hexane, ethyl acetate and butanol extracts of the sea-cucumber Isostichopus badionotus (Holothuroidea) was tested against normal cells (Vero), human cervical carcinoma (HeLa) and breast adenocarcinoma (MCF-7 and MDA-MB-231) ATCC cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Hexane extracts from body walls and viscera showed high cytotoxic activity against HeLa cells (IC50’s = 48.5 and 42.5 µg mL-1, respectively), while the ethyl acetate extract of body walls was considered low active (IC50 = 98.3 μg mL-1). In addition, the body walls hexane extract showed a good selectivity index value of 12.0.


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Copyright (c) 2014 Aida R. Pérez-Espadas, María J. Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cárdenas, María E. Morales-Rubio, Lorena V. León-Deniz, Jaqueline Canul-Canché , Leovigildo Quijano