Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect.
Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal’s burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against -2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked.
In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 109CFU/mL of S. aureus.
Jorge MP, Madjarof C, Ruiz ALTG, Fernandes AT, Rodrigues RAF, Sousa IMO, Foglio MA, Ernesto De Carvalho J. Evaluation of wound healing properties of Arrabidaeachica Verlot extract. J Ethnopharmacol 2008; 118: 361-366. http://dx.doi.org/10.1016/j.jep.2008.04.024
Alemdaroglu C, Degim Z, Celebi N, Zor F, Ozturkand S, Erdogan D. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns 2006; 32: 319-327. http://dx.doi.org/10.1016/j.burns.2005.10.015
Pereira DST, Lima-Ribeiro MHM, Nicodemos TPF, Carneiro-Leao AMA, Correia MTS. Development of Animal Model for Studying Deep Second-Degree Thermal Burns. J Biomed Biotechnol 2012a; 2012: 7 p. http://dx.doi.org/10.1155/2012/460841
Gurung S, Skalko-Basnet N. Wound healing properties of Carica papaya latex: In vivo evaluation in mice burn model. J Ethnopharmacol 2009; 121: 338-341. http://dx.doi.org/10.1016/j.jep.2008.10.030
Darré T, Metowogo K, Lawson-Evi P, Eklu-Gadegbeku K, Aklikokou AK, Napo-Koura G, Gbeassor M. Effet topique de l’extrait hydro-ethanolique de l’acacia nilotica sur la cicatrisation cutanée des brulures de second degre. European Scientific Journal 2014; 10: 1857-7881.
Trombetta D, Puglia C, Perri D, Licata A, Pergolizzi S, De Pasquale A, Saija A, Bonina FP. Effect of polysaccharides from Opuntiaficus-indica (L.) clatodes on the healing of dermal wounds in the rat. Phytomedicine 2006; 13: 352-358. http://dx.doi.org/10.1016/j.phymed.2005.06.006
Amegbor K, Metowogo K, Eklu-Gadegbeku K, Agbonon A, Aklikokou AK, Napo-Koura G, Gbeassor M. Preliminary evaluation of the wound healing effect of vitexdonianasweet (verbenaceae) in mice. Afr J Tradit Complement Altern Med 2012; 9: 584-590. http://dx.doi.org/10.4314/ajtcam.v9i4.16
Kumar MS, Kirubanandan S, Sripriya R, Sehgal PK. Triphala Promotes Healing of Infected Full-Thickness Dermal Wound. J Surg Res 2008; 144: 94-101. http://dx.doi.org/10.1016/j.jss.2007.02.049
Sadaf F, Saleem R, Ahmed M, Ahmadand SI, Navaid-Ul Z. Healing potential of cream containing extract of Sphaeranthusindicius on dermal wounds in Guinea pigs. J Ethnopharmacol 2006; 107: 161-163. http://dx.doi.org/10.1016/j.jep.2006.02.022
Mcmanus JFA, Mowry RW. Stanning methods, histologic and histochemical. Haper Raw, New York, Evanston, Lodon 1965.
Akkol EK, Koca U, Pe Sin I, Yilmazer D, Tokerand G, Yesilada E. Exploring the wound healing activity of Arnebiadensiflora (Nordm.) Ledeb by in vivo models. J Ethnopharmacol 2009; 124: 137-141. http://dx.doi.org/10.1016/j.jep.2009.03.019
Priya KS, Gnanamani A, Radhakrishnan N, Babu M. Healing potential of Datura alba on burn wounds in albino rats. J Ethnopharmacol 2002; 83: 193-199. http://dx.doi.org/10.1016/S0378-8741(02)00195-2
Mokaddas EVO, Sanyal SC. In vitro activity of piperacillin/tazobactam versus other broad antibiotics against nosocomial gram negative pathogens isolated from burn patients. J Chemotherapy 1998; 10: 208-214. http://dx.doi.org/10.1179/joc.19184.108.40.206
Kumar MS, Kirubanandan S, Sripriya R, Raghavan HV, Sehgal PK. Wound Healing Potential of Cassia fistula on Infected Albino Rat Model. J Surg Res 2006; 131: 283-289. http://dx.doi.org/10.1016/j.jss.2005.08.025
Pereira DST, Lima-Ribeiro MHM, Santos-Oliveira R, Cavalcanti CLB, Teles De Pontes-Faldo N, Coelho LCBB, Carneiro-Leao AMA, Correia MTS. Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model. J biomed biotechnol 2012b; 2012: 11p. http://dx.doi.org/10.1155/2012/184538
Arias ME, Gomez JD, Cudmani NM, Vattuone MA, Isla MI. Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. ex Hook et Arn. Life Scie 2004; 75: 191-202. http://dx.doi.org/10.1016/j.lfs.2003.12.007
Al-Fatimi M, Wurster M, Schroder G, Lindequist U. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. J Ethnopharmacol 2007; 111: 657-666. http://dx.doi.org/10.1016/j.jep.2007.01.018
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2015 Kossi Metowogo, Tchin Darré, François Daubeuf, Povi Lawson-Evi, Kwashi Eklu-Gadegbeku, Kodjo A. Aklikokou, Gado Napo-Koura, Nelly Frossard , Messanvi Gbeassor