Beetroot Juice Supplementation Increases High Density Lipoprotein-Cholesterol and Reduces Oxidative Stress in Physically Active Individuals


 Antioxidants,Lipid profile,Cortisol, Supplementation, Cardiovascular health.

How to Cite

Archana Singh, Saroj Kumar Verma, Vijay Kumar Singh, Chindalaga Nanjappa, Nagraj Roopa, Pakalpati Srinivas Raju, & Som Nath Singh. (2015). Beetroot Juice Supplementation Increases High Density Lipoprotein-Cholesterol and Reduces Oxidative Stress in Physically Active Individuals. Journal of Pharmacy and Nutrition Sciences, 5(3), 179–185.


Beetroot juice contains a high level of biologically accessible antioxidants, beneficial phytochemicals and dietary nitrate, which seem to exert beneficial effects in human health. Dietary nitrate, from beetroot has been reported to lower blood pressure. However the impact of beetroot on lipid profile and oxidative stress is unknown. In present study, the effect of supplementation with beetroot juice for 15 days was investigated. Plasma lipid profile, antioxidant status, oxidative stress and body composition changes were evaluated at baseline and after 15 days of beetroot juice supplementation. Beetroot juice supplementation beneficially influenced the lipid profile by significantly increasing the levels of high-density lipoprotein cholesterol (HDL-C) from 42.9 ± 8.3 mg/dl to 50.2 ± 9.8 mg/dl and decreasing low-density lipoprotein cholesterol (LDL-C) from 129.7 ± 82.3 mg/dl to 119.5 ± 79.2 mg/dl compared with baseline values. Beetroot juice supplementation increased (P < 0.05) plasma nitrite level and guanosine 3’, 5’-cyclic monophosphate (c-GMP) levels. A significant increase in plasma total antioxidant capacity and vitamin C levels was observed after beetroot juice intake for 15 days. There was no significant change in the body fat mass and lean body mass of participants with the beetroot juice supplementation. Beetroot juice supplementation significantly decreased the stress markers plasma hydroperoxides and cortisol levels. Beetroot juice acts as a potent vasodilator by increasing plasma c-GMP levels and nitrite levels. Beetroot juice consumption improves plasma lipid profile and antioxidant status, encouraging further evaluation on a population with higher cardiovascular disease risk.


Bhupathiraju SN, Wedick NM, Pan A, et al. Quantity and variety in fruit and vegetable intake and risk of coronary heart disease. American Journal of Clinical Nutrition 2013; 98(6): 1514-23.

Ruel G, Shi Z, Zhen S, et al. Association between nutrition and the evolution of multimorbidity: The importance of fruits and vegetables and whole grain products. Clinical Nutrition 2014; 33(3): 513-20.

Hartley L, Igbinedion E, Holmes J, et al. Increased consumption of fruit and vegetables for the primary prevention of cardiovascular diseases. Cochrane Database of Systematic Reviews 2013; 6: 1469-493.

Sluik D, Boeing H, Li K, et al. Lifestyle factors and mortality risk in individuals with diabetes mellitus: are the associations different from those in individuals without diabetes? Diabetologia 2014; 57(1): 63-72.

Crane TE, Khulpateea BR, Alberts DS, Basen-Engquist K, Thomson CA. Dietary Intake and Ovarian Cancer Risk: A Systematic Review. Cancer Epidemiology, Biomarkers and Prevention 2014; 23(2): 255-73.

Marchioli R, Schweiger C, Levantesi G, Tavazzi L, Valagussa F. Antioxidant vitamins and prevention of cardiovascular disease: epidemiological and clinical trial data. Lipids 2001; 36: S53-63.

Cosby K, Partovi KS, Crawford JH, et al. Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nature Medicine 2003; 9(12): 1498-1505.

Webb AJ, Patel N, Loukogeorgakis S, et al. Acute blood pressure lowering, vasoprotective, and antiplatelet properties of dietary nitrate via bioconversion to nitrite. Hypertension 2008; 51, 784–790

Wootton-Beard PC, Moran A, Ryan L. Stability of the antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion as measured by FRAP, DPPH, ABTS and Folin Ciocalteu methods. Food Research International 2011; 44: 217-224.

Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology 2002; 37: 153-161.

Pitalua E, Jimenez M, Vernon-Carter EJ, Beristain CI. Antioxidative activity of microcapsules with beetroot juice using gum Arabic as wall material. Food and Bioproducts Processing 2010; 88: 253-258.

Lansley KE, Winyard PG, Bailey SJ, et al. Acute dietary nitrate supplementation improves cycling time trial performance. Medicine and Science in Sports and Exercise 2011; 43(6): 1125-1131.

Miller GD. Beets Go Mainstream. Nutritional Disorders & Therapy 2013; 3: 3.

Hord NG, Tang Y, Bran NS. Food sources of nitrates and nitrites: the physiologic context for potential health benefits. American Journal of Clinical Nutrition 2009; 90(1): 1-10.

Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science 1993; 84: 407-412.

Zannoni V, Lynch M, Goldstein S, Sato P. A rapid micro method for the determination of ascorbic acid in plasma and tissues. Biochemical Medicine 1974; 11(1): 41-48.

Wolff SP. Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods in Enzymology 1994; 233: 182-189.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 1951; 193: 265.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry 1972; 18: 499-502.

Bailey SJ, Fulford J, Vanhatalo A, et al. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. Journal of Applied Physiology 2010; 109(1): 135-148.

Heunks LM, Cody MJ, Geiger PC, Dekhuijzen PN, Sieck GC. Nitric oxide impairs Ca2± activation and slows cross-bridge cycling kinetics in skeletal muscle. Journal of Applied Physiology 2001; 91(5): 2233-2239.

Galler S, Hilber K, Göbesberger A. Effects of nitric oxide on force-generating proteins of skeletal muscle. PflugersArchiv 1997; 434(3): 242-245.

Hobbs AJ. Soluble guanylate cyclase: the forgotten sibling. Trends in Pharmacological Sciences 1997; 18: 484-491.

Baliga RS, Milsom AB, Ghosh SM, et al. Dietary nitrate ameliorates pulmonary hypertension: cytoprotective role for endothelial nitric oxide synthase and xanthine oxidoreductase. Circulation 2012; 125(23): 2922-2932.

Viner RI, Williams TD, Schöneich C. Nitric oxide-dependent modification of the sarcoplasmic reticulum Ca-ATPase: localization of cysteine target sites. Free Radical Biology and Medicine 2000; 29(6): 489-496.

Lopez AD, Mathers CD, Ezzati M, Jamison DT, Murray CJL. Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data. Lancet 2006; 367: 1747-57.

Bazzano LA, He J, Ogden LG, et al. Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study. American Journal of Clinical Nutrition 2002; 76: 93-9.

Joshipura KJ, Ascherio A, Manson JE, et al. Fruit and vegetable intake in relation to risk of ischemic stroke. Journal of the American Medical Association 1999; 282: 1233-9.

Joshipura KJ, Hu FB, Manson JE, et al. The effect of fruit and vegetable intake on risk for coronary heart disease. Annals of Internal Medicine 2001; 134: 1106-14.

Ness AR, Powles J. Fruit and vegetables, and cardiovascular disease: a review. International Journal of Epidemiology 1997; 26: 1-13.

Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002; 18: 872-879.

Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003; 189: 41-54.

Escribano J, Pedreño MA, García-Carmona F, Muñoz R. Characterization of the antiradical activity of betalains from beta vulgaris L. roots. Phytochemical Analysis 1998; 9(3): 124-127.<124::AID-PCA401>3.0.CO;2-0

Aprikian O, Levrat-Verny M, Besson C, Busserolles J, Rémésy C, Demigné C. Apple favourably affects parameters of cholesterol metabolism and of anti-oxidative protection in cholesterol-fed rats. Food Chemistry 2001; 75: 445-452.

Lee JH, Felipe P, Yang YH, et al. Effects of dietary supplementation with red-pigmented leafy lettuce (Lactuca sativa) on lipid profiles and antioxidant status in C57BL/6J mice fed a high-fat high-cholesterol diet. British Journal of Nutrition 2009; 101: 1246-1254.

Kanner J, Harel S, Granit R. Betalains – a new class of dietary cationized antioxidants. Journal of Agricultural and Food Chemistry 2001; 49(11): 5178-5185.

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Copyright (c) 2015 Archana Singh, Saroj Kumar Verma, Vijay Kumar Singh, Chindalaga Nanjappa, Nagraj Roopa, Pakalpati Srinivas Raju , Som Nath Singh