Inhibitory Effects of Leaves of Guava (Psidium guajava) on TPA-Induced Inflammation and Tumor Promotion in Two-Stage Carcinogenesis in Mouse Skin

Authors

  • Ken Yasukawa Nihon University, 7-7-1, Narashinodai, Chiba 274-8555, Japan
  • Tomohito Kakegawa JosaiInternationalUniversity, 1Gumyo, Togane, Chba283-8555 ,Japan

DOI:

https://doi.org/10.6000/1927-5951.2015.05.04.1

Keywords:

 Guava, Psidium guajava, antitumor promotion, anti-inflammation, cancer prevention.

Abstract

Cancer prevention offers the most cost-effective long-term health strategy. The methanol extract of the leaves of guava(Psidium guajava) exhibits marked antitumor activity in an in vivo two-stage carcinogenesis test in mice using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and 12-O-tetradecanoylphorbol-13- acetate (TPA) as a promoter. From the active fraction of the methanol extract, five triterpene acids, uvaol (1), ursolic acid (2), corosolic acid (3), asiatic aci (4), and oleanolic acid d (5), were isolated and identified. These compounds were evaluated for their inhibitory effects on TPA-induced inflammation (1 µg/ear) in mice, and showed marked anti-inflammatory effects, with a 50% inhibitory dose of 117–657 nmol/ear. The leaves of guava may therefore be effective for cancer prevention.

References

Yasukawa K. Edible and medicinal plants as cancer chemopreventive agents, In: ‘Drug discovery research in pharmacognosy’. Eds Vallisuta O, Olimat SM. InTech, Rijeka 2012; pp. 181‒208. http://dx.doi.org/10.5772/34545

Yasukawa K. Edible and medicinal mushrooms as promising agents in cancer. In: ‘Drug discovery and development ― From molecules to medicine’, Eds Vallisuta O, Olimat SM. InTech, Rijeka 2015; pp. 39‒61. http://dx.doi.org/10.5772/59964

Yasukawa K. Cancer chemopreventive agents: Natural pentacyclic triterpenoids. In: ‘Pentacyclic triterpenes as promising agents in cancer’. Ed Salvador JAR. Nova Science Publishers, New York 2010; pp. 127‒157. https://www.novapublishers.com/catalog/product_info.php?products_id=12372

Yasukawa K. Cancer chemopreventive agents: tetracyclic triterpenes promising as cancer, In: ‘Horizons in cancer research. Vol. 51’ Ed Watanabe HS, Nova Science Publisher, New York 2011; pp. 89‒113. https://www.novapublishers.com/catalog/product_info.php?products_id=42026&osCsid=b91c93eb69fe4df43fab8c1d356c0176

Gutiérrez RMP, Mitchell S, Solis RV. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 2008; 117: 1‒27. http://dx.doi.org/10.1016/j.jep.2008.01.025

Sanda KA, grema HA, Geidam YA, Bukar-Kolo YM. Pharmacological aspects of Psidium guajava: an update. Int J Pharmacol 2011; 7: 316-324. http://dx.doi.org/10.3923/ijp.2011.316.324

Mahato SB, Kundu AP. 13C NMR spectra of pentacyclic triterpenoids ―A compilation and some salient features. Phytochemistry 1994; 37: 1517-1575. http://dx.doi.org/10.1016/S0031-9422(00)89569-2

Li L-M, Pu J-X, Xiao W-L, Sun H-D. Triterpenes from Isodon xerophilus. Chinese J Nat Med 2012; 10: 307-310. http://dx.doi.org/10.3724/SP.J.1009.2012.00307

Cheng J-J, Zhang L-J, Cheng H-L, Chiou C-T, Lee I-J, Kuo Y-H. Cytotoxic hexacyclic triterpene acids from Euscaphis japonica. J Nat Prod 2010; 73: 1655-1658. http://dx.doi.org/10.1021/np1003593

Yasukawa K, Takahashi H, Kitanaka S, Hirayama H, Shigemoto K. Inhibitory effect of an aqueous extract of Phellinus linteus on tumor promotion in mouse skin. Mushroom Sci Biotechnol 2007; 15: 97-101.

Akita A, Sun Y, Yasukawa K. Inhibitory effects of chaga (Inonotus obliquus) on tumor promotion in two-stage mouse skin carcinogenesis. J Pharm Nutr Sci 2015; 5: 71-76. http://dx.doi.org/10.6000/1927-5951.2015.05.01.11

Yasukawa K, Sun Y, Kitanaka S, Tomizawa N, Miura M, Motohashi S. Inhibitory effect of the Rhizomes of Alpinia officinarum on TPA-induced inflammation and tumor promotion in two-stage carcinogenesis in mouse skin. J Nat Med 2008; 62: 374-378. http://dx.doi.org/10.1007/s11418-008-0243-2

Yasukawa K, Yu SY, Tsutsumi S, Kurokawa M, Park YK. Inhibitory effects of Brazilian propolis on tumor promotion in two-stage mouse skin carcinogenesis. J Pharm Nutr Sci 2012; 2: 71-76. http://dx.doi.org/10.6000/1927-5951.2012.02.01.10

Yasukawa K, Okuda S, Nobushi Y. Inhibitory effect of gymnema (Gymnema sylvestre) leaves on tumour promotion in two-stage mouse skin carcinogenesis. Evid-Based Complement Alternat Med 2014; 2014: 328684. http://dx.doi.org/10.1155/2014/328684

Yasukawa K, Kitanaka S, Kawata K, Goto K. Anti-tumor promoters phenolics and triterpenoid from Hippophae rhamnoides. Fitoterapia 2009; 80: 164-167. http://dx.doi.org/10.1016/j.fitote.2009.01.006

Yasukawa K, Takido M, Takeuchi M, Nakagawa S. Effect of chemical constituents from plants on 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice. Chem Pharm Bull (Tokyo) 1989; 37: 1071-1073. http://dx.doi.org/10.1248/cpb.37.1071

Huang M-T, Ho C-T, Wang Z-Y, Ferraro T, Lou Y-R, Stauber K, Ma W, Georgiadis C, Laskin JD, Conney AH. Inhibition of skin tumorigenesis by rosemary and its constituents carnosol and ursolic acid. Cancer Res 1994; 54: 701‒708. http://cancerres.aacrjournals.org/content/54/3/701.long

Tokuda H, Ohigashi H,Koshimizu K,Ito Y. Inhibitory effects of ursolic and oleanolic acid on skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate. Cancer Letters 1986; 33: 279-285. http://dx.doi.org/10.1016/0304-3835(86)90067-4

Ringborn T, Segura L, Noreen Y, Perera P, Bohlin L. Ursolic acid from Plantago major, a selective inhibitor of cyclooxygenase-2 catalyzed prostaglandin biosynthesis. J Nat Prod 1998; 61: 1212‒1215. http://dx.doi.org/10.1021/np980088i

Subbaramaiah K, Michaluarth P, Sporn MB, Annenberg AJ. Ursolic acid inhibits cyclooxigenese-2 transcription in human mammary epithelial cells. Cancer Res 2000; 60: 2399‒2404. http://cancerres.aacrjournals.org/content/60/9/2399.long

You HJ, Choi CY, Kim JY, Park SJ, Hahm KS, Jeong HG. Ursolic acid enhances nitric oxide and tumor necrosis factor-α production via nuclear factor-κB activation in the resting macrophages. FEBS Lett 2001; 509: 156‒160. http://dx.doi.org/10.1016/S0014-5793(01)03161-1

Cha HJ, Park MT, Chung HY, Kim ND, Sato H, Seiki M, Kim K-W. Ursolic acid-induced down-regulation of MMP-9 gene is mediated through the nuclear translocation of glucocorticoid receptor in HT1080 human fibrosarcoma cells. Oncogene 1998; 16: 771‒778. http://dx.doi.org/10.1038/sj.onc.1201587

Yim E-K, Lee K-H, Namkoong S-E, Um S-J, Park J-S. Proteomic analysis of ursolic acid-induced apoptosis in cervical carcinoma cells. Cancer Lett 2006; 235: 209‒220. http://dx.doi.org/10.1016/j.canlet.2005.04.007

Fujiki H, Sueoka E, Suganuma M. Tumor promoters: from chemicals to inflammatory proteins. J Cancer Res Clin Oncol 2013; 139: 1603‒1614. http://dx.doi.org/10.1007/s00432-013-1455-8

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Published

2015-08-05

How to Cite

Ken Yasukawa, & Tomohito Kakegawa. (2015). Inhibitory Effects of Leaves of Guava (Psidium guajava) on TPA-Induced Inflammation and Tumor Promotion in Two-Stage Carcinogenesis in Mouse Skin . Journal of Pharmacy and Nutrition Sciences, 5(4), 216–221. https://doi.org/10.6000/1927-5951.2015.05.04.1

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