Physical and Chemical Mutation for Enhanced Alpha-Amylase Production by Aspergillus fumigatus NTCC1222 under Solid State Fermentation Conditions Using Agri-Residue Waste


Aspergillus fumigatus, Amylase, UV-mutation, EMS-EtBr mutation.

How to Cite

Shalini Singh, Sanamdeep Singh, & Jyoti Mangla. (2016). Physical and Chemical Mutation for Enhanced Alpha-Amylase Production by Aspergillus fumigatus NTCC1222 under Solid State Fermentation Conditions Using Agri-Residue Waste . Journal of Pharmacy and Nutrition Sciences, 6(1), 22–26.


The search for better microbial sources of enzymes has long been an area of active research owing to the clear and distinct importance in todays’ world where exploration and application of environment friendly products has become a necessity. Though the natural, wild microbial strains, are continuously be searched and explored for their potential for amylase production yet, simultaneous attempts for improved enzyme production are necessary too. In the present study, a comparison between the influence of different mutational treatments (UV treatment and EMS-EtBr treatment) on amylase production was studied. Aspergillus fumigatus NTCC1222, an indigenously isolated amylase producer, which has shown an amylase activity of 341.7 U/mL under optimized conditions of SSF in our previous study, was subjected to UV- and EMS-EtBr- treatment for possible improved amylase production under optimized solid state fermentation conditions. The UV mutated strains yielded an amylase production of 614.2 U/mL while the chemically (EMS-EtBr) mutated strain produced 814.1 U/mL of amylase activity thereby indicating the successful enhancement in amylase activity for the test fungal strain.


Payen A. Memoir on the composition of the tissue of plants and of woody [material

Kirk O, Borchert TV, Fuglsang CC. Industrial enzyme applications. Current Opinion in Biotechnology 2002; 13: 345-351.

Singh S, Tyagi CH, Dutt D, Upadhyaya JS. Production of high level of cellulase-poor xylanases by wild strains of white rot fungus Coprinellus disseminatus in solid state fermentation. New Biotechnology 2009; 26(¾): 165-170.

Singh S, Dutt D. Mitigation of adsorbable organic halides in combined effluents of wheat straw soda-AQ pulp bleached with cellulase-poor crude xylanases of Coprinellus disseminatus in elemental chlorine free bleaching. Cellulose chemistry and Technology 2014; 48(1-2): 127-135.

Singh S, Sharma S, Kaur C, Dutt D. Potential of cheap cellulosic residue as carbon source in amylase production by Aspergillus niger SH-2 for application in enzymatic desizing at high temperatures. Cellulose Chemistry and Technology 2014; 48(5-6): 521-527.

Prabakaran M, Thennarasu V, Mangala RA, Bharathidasan R, Chandrakala N, Mohan N. Comparative studies on the enzyme activities of wild and mutant fungal strains isolated from sugarcane field. Indian Journal of Science and Technology 2009; 2(11): 46-49.

Singh S, Dutt D, Tyagi CH. Screening of Xylanases from Indigenously Isolated White Rot Fungal Strains for Possible Application in Pulp Biobleaching. Open Access Scientific Reports 2013; 2(602).

Singh S, Dutt D, Tyagi CH. Environmentally friendly total chlorine free bleaching of wheat straw pulp using novel cellulase poor xylanases of wild strains of Coprinellus disseminatus. Bioresources 2011; 6(4): 3876-3882.

Singh S, Dutt D, Tyagi CH, Upadhyaya JS. Bio-conventional bleaching of wheat straw soda-AQ pulp with crude xylanases from SH-1 NTCC-1163 and SH-2 NTCC-1164 strains of Coprinellus disseminatus to mitigate AOX generation. New Biotechnology 2010; 28(1): 47-57.

Tyagi CH, Singh S, Dutt D. Effect of two fungal strains of Coprinellus disseminatus SH-1 NTCC-1163 and SH-2 NTCC-1164 on pulp refining and mechanical strength properties of wheat straw soda-AQ pulp. Cellulose Chemistry and Technology 2011; 45(3-4): 257-263.

Kar S, Ray RC, Mohapatra UB. Alpha-amylase production by Streptomyces erumpens MTCC 7317 in solid state fermentation using response surface methodology (RSM). Polish Journal of Microbiology 2008; 57(4): 289-296.

Champreda V, Kanokratana P, Sriprang R, Tanapongpipat S, Eurwilaichitr L. Purification, biochemical characterization, and gene cloning of a new extracellular thermotolerant and glucose tolerant maltooligosaccharide-forming alpha-amylase from an endophytic ascomycete Fusicoccum sp. BCC4124. Bioscience, Biotechnology and Biochemistry 2007; 71(8): 2010-2020.

Sindhu R, Suprabha GN, Shashidhar S. Optimization of process parameters for the production of ?- amylase from Penicillium janthinellum (NCIM 4960) under solid state fermentation. African Journal of Microbiology Research 2009; 3(9): 498-503.

Prabhakar A, Krishnaiah K, Janaun J, Bono A. An overview of engineering aspects of solid state fermentation. Malaysian Journal of Microbiology 2005; 1(2): 10-16.

Singh S, Sharma S, Kaur C, Dutt D. UV-and EMS-induced mutations affecting synthesis of alkaloids and lipase in Penicillium roquefortii. Cell Chem and Technol 2014; 48(5-6): 521-527.

EL-Bondkly AM, Keera AA. Arab J Biotech 2007; 10(2): 241-248.

Chadha BS, Rubinder K, Saini HS. Constitutive ?-amylase producing mutant and haploid strains of thermophillic fungus Thermomyces lanuginosus. Folia Microbial 2005; 50(2): 133-140.

Hanh VV, Pham TA, Kim K. Improvement of fungal cellulase production by mutation and optimization of solid state fermentation. Mycobiology 2011; 39(1): 20-25.

Bin G, Laisu X, Youfang D, Yanquan L. Screening of alpha amylase high-producing strains from Bacillus subtilis. Journal of Zhejiang 1999; 23: 88-92.

Feitkenhauer H. Anaerobic digestion of desizing wastewater: influence of pretreatment and anionic surfactant on degradation and intermediate accumulation. Enzyme and Microbial Technology 2003; 33: 250-258.

Gigras P, Sahai V, Gupta R. Statistical Media optimization and Production of ITS ?-Amylase from Aspergillus oryzae in a bioreactor. African Journal of Microbiology Research 2002; 45: 203-208.

Hmidet N, El-Hadj Ali N, Haddar A, Kanoun S, Alya S, Nasri M. Alkaline proteases and thermostable ?-amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive. Biochemical Engineering Journal 2009; 47: 71-79.

Varalakshmi KN, Kumudini BS, Nandini BN, Solomon J, Suhas R, Mahesh B, Kavitha AP. Production and characterzition of ?- Amylase from Aspergillus niger JGI24 isolated in Bangalore. Polish Journal of Microbiology 2009; 58(1): 29-36.

Singh S, Singh S, Bali V, Sharma L, Mangla J. Production of fungal amylases using cheap, readily available agriresidues, for potential application in textile industry. BioMed Research International 2014; 9(14).

Singh S, Cheema SK, Kaur B, Mann NK. Influence of ethanol on growth alpha-amylase production for Aspergillus fumigatus NTCC1222 under solid state fermentation. International Journal of Engineering Research and Technology 2013; 6(8): 67-69.

Singh S, Cheema SK, Kaur B, Mann NK. Sorbitol: an enhancer of growth and alpha-amylase production for Aspergillus fumigatus NTCC1222 using wheat bran as substrate. International Journal of Biotechnology and Bioengineering Research 2013; 4(6): 555-560.

Singh S, Kaur B, Mann NK, Cheema SK. Influence of calcium chloride on growth and alpha-amylase production for wild and UV-mutated strains of Aspergillus fumigatus. International Journal of Biotechnology and Bioengineering Research 2013; 4(7): 697-702.

Miller GL, Analytical Chemistry, Use of dinitro salicylic acid reagent for determination of reducing sugar 1959; 31: 426-429.

Sowmiya E, Jagannathan N, Rajagopal K. Strain Improvement Of Curvularia lunata to enhance the lipid production by chemical mutagenesis. International Journal of Pharma and Bio Sciences 2011; 2(3): 9-27.

Zia MA, Rahman K, Sheikh MA, Khan IA. Chemically treated strain improvement of Aspergillus niger for enhanced production of glucose oxidase. International Journal Of Agriculture & Biology 2010; 10(407): 964-966.

Nicolás-Santiago SD, Regalado-González C, García-Almendárez B, Fernández FJ, Téllez-Jurado A, Huerta-Ochoa S. Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants. Electronic J Biotechnol 2006; 9: 10-19.

Suribabu K, Govardhan TL, Hemalatha KPJ. Strain Improvement of Brevibacillus borostelensis R1 for Optimization of ?-Amylase Production by Mutagens. J Microb Biochem Technol 2014; 6(3): 123-127.

Meenu M, Santhosh D, Kamia C, Randhir S. Strain improvement of Aspergillus flavus for enhanced production. Ind J Microbiol 2000; 40: 25-32.

Sarikaya E, Gürgün V. Increase of the a-amylase yield by some Bacillus strains. Turk J Biol 2000; 24: 299-308.

Ikram-ul-haq S, Ali A, Saleem JMM. Mutagenesis Of Bacillus licheniformis through Ethyl Methanesulfonate for alpha Amylase Production. Pak J Bot 2009; 41: 1489-1498.

McCann J, Ames BN. Detection of carcinogens as mutagens in the Salmonella/microsome test:assay of 300 chemicals. Proc Natl Acad Sci USA 1975; 72: 5135-5139.

Sathyaprabha G, Panneerselvam A, Muthukkumarasamy S. Production of cellulase and amylase from wild and mutated fungal isolates. E-Journal of Life Sciences 2011; 1(1): 39-45.

Malik S, Iftikhar T, Ul Haq I. Enhanced amyloglucosidase biosynthesis through mutagenesis using Aspergillus niger. Pak J Bot 2011; 43(1): 111-119.

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Copyright (c) 2016 Shalini Singh, Sanamdeep Singh , Jyoti Mangla