Use of Information Parameters as Criterion for Determination of Biological Activity of Hepatoprotective Preparations


  • David Areshidze Moscow State Regional University, 10A Radio st., Moscow, Russian Federation
  • Lyudmila Timchenko North Caucasus Federal University, 1 Pushkin st., Stavropol, Russian Federation
  • Maria Kozlova Moscow State Regional University, 10A Radio st., Moscow, Russian Federation
  • Igor Rzhepakovsky North Caucasus Federal University, 1 Pushkin st., Stavropol, Russian Federation
  • Ivan Syomin Moscow State Regional University, 10A Radio st., Moscow, Russian Federation



 Liver, hepatoprotector, biological activity, entropy.


Possibility of use of the information parameters characterizing adaptational and regenerative opportunities of tissue system of an organ as potential criteria for an assessment of biological activity of hepatoprotective preparations is investigated in this research. Influence of enzymatic hydrolyzate of Chlorophytum comosum (L.) on a morphofunctional condition of a liver of rats at experimental toxic damage of organ and in norm was studied. The assessment of morphofunctional condition of a liver under the influence of a preparation was carried out as with use of traditional morphological, biochemical and histologic parameters, and by means of information parameters, which were earlier used for an evaluation of the adaptative and regenerative opportunities of organs of mammals. As a result of research the hepatoprotective effect of enzymatic hydrolyzate of Chlorophytum comosum (L.) is confirmed both by dynamics of change of results of traditional techniques of an assessment and by informational parameters, that allows to draw a conclusion on possibility of use of information parameters as criterion for assesment of effectiveness of biostimulation.


Brodanova M. Etiology and epidemiology of hepatocellular carcinoma. Vnitr Lek 1997; 43(3): 157-161.

Emerit J, Samuel ?, Pavio N. Cu-Zn super oxide dismutase as a potential antifibrotic drug for hepatitis Crelated fibrosis. Biomed Pharmacother 2006; 60: 1-4.

Kala CP, Dhyani BS, Sajwan BS. Developing the medicinal plants sector in northern India: challenges and opportunities. J Ethnobiol Ethnomed 2006; 6: 32-38.

Morisco F, Vitaglione P, Amoruso D, Russo B, Fogliano V, Caporaso N. Foods and liver health. Mol Aspects Med 2008; 29(1-2): 144-150.

Guy J, Peters MG. Liver disease in women: the influence of gender on epidemiology, natural history, and patient outcomes. Gastroenterol Hepatol (NY) 2013; 9(10): 633-639.

Foxcroft DR, Coombes L, Wood S, et al. WITHDRAWN: Motivational interviewing for alcohol misuse in young adults. Cochrane Database Syst Rev 9. CD007025.

Ghajarieh Sepanlou S, Malekzadeh F, Naqhavi M, et al. Trend of gastrointestinal and liver diseases in Iran: results of the Global Burden of Disease study, 2010. Middle East J Dig Dis 2015; 7: 121-137.

Iruzubieta P, Teran ?, Crespo J, Fabrega E. Vitamin D deficiency in chronic liver disease. World J Hepatol 2014; 6(12): 901-915.

Pellegrini M, Pallottini V, Marin R, et al. Role of sex hormone estrogen in the prevention of lipid disorder. Curr Med Chem 2014; 21(24): 2734-2742.

Wieland A, Frank DN, Harnke B. Systematic review: microbial dysbiosis and nonalcoholic fatty liver disease. Aliment Pharmacol Ther 2015; 42(9): 1051-1063.

Kandilis AN, Papadopoulou IP, Koskinas J, Sotiropoulos G, Tiniakos DG. Liver innervation and hepatic function: new insights. J Surg Res 2015; 194(2): 511-519.

McMorrow AM, Connaughton RM, Lithander FE, Roche HM. Adipose tissue dysregulation and metabolic consequences in childhood and adolescent obesity: potential impact of dietary fat quality. Proc Nutr Soc 2015; 74(1): 67-82.

Yi J,Xia W, Wu J, Yuan J, et al. Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice. J Vet Sci 2014; 15: 141-148.

Kuntz E, Kuntz H-D. Hepatology: Principles and Practice Springer Medizin Verlag Heidelberg, 2006: 889-902.

Ramawat KG, Merillon J-M. Bioactive molecules and medicinal plants. Springer-Verlag Berlin Heidelberg 2008: 391.

Benzie IFF, Wachtel-Galor S. Herbal medicine: biomolecular and clinical. – 2nd edition. – Taylor & Francis Group 2011: 488.

Ramawat KG. Herbal drugs: Ethnomedicine to modern medicine. Springer-Verlag Berlin Heidelberg 2009; 414.

Kolotilin I, Topp E, Cox B, et al. Plant-based solutions for veterinary immunotherapeutic and prophylactic. Vet Res 2014; 45(117): 1-12.

Zhou Y-X, Xia W, Yue W, et al. Rhein: a review of pharmacological activities. Evid based Complement Alternat Med 2015; 1-10.

Salas CE, Badillo-Corona JA, Ramirez-Sotelo G. Biologically active and antimicrobial peptides from plants. Biomed Res Int 2015; 1-12.

Osbourn AE, Lanzotti V. Plant-derived natural products: synthesis, function, and application. Springer Science+Business Media 2009: 598.

Hermenean A, Popescu ?, Ardelean A, et al. Hepatoprotective effects of Berberis vulgaris L. extract/? cyclodextrin on carbon tetrachloride-induced acute toxicity in mice. Int J Mol Sci 2012; 13(7): 9014-9034.

Iloon Kashkooli R, Najafi SS, Sharif F, et al. The effect of berberis vulgaris extract on transaminase activities in non-alcoholic Fatty liver disease. Hepat Mon 2015; 15(2): e25067.

Oqus S, Kanter M, Erboga M, Irbis C. Protective effect of Urtica dioica on liver damage induced by biliary obstruction in rats 2013; 29(9): 838-845.

Liu JH, Tang J, Zhu ZF, et al. Design, synthesis, and anti-tumor activity of novel betulinic acid derivatives. J Asian Nat Prod Res 2014; 16: 34-42.

Mohamed MA, Marzouk MS, Moharram MM, et al. Phytochemical constituents and hepatoprotective activity of Viburnum tinus. Phytochemistry 2005; 66(23): 2780-2786.

Madi Almajwal A, Farouk Elsadek M. Lipid-lowering and hepatoprotective effects of Vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats. Nutr Res Pract 2015; 9: 37-42.

Georgiev V, Ananga A, Tsolova V. Recent advances and uses of grape flavonoids as nutraceuticals. Nutrients 2014; 6: 391-415.

Giribabu N, Eswar Kumar K, Swapna Rekha S. Vitis vinifera (Muscat variety) seed ethanolic extract preserves activity levels of enzymes and histology of the liver in adult male rats with diabetes. Evid based Compl Alternative Med 2015: 1-8.

Czinner E, Hagymasi, Blazovics A. The in vitro effect of Helichrysi flos on microsomal lipid peroxidation. J Ethnopharmacol 2001; 77(1): 31-35.

Paul A, Das J, Samadder A, et al. Poly (lactide-co-glycolide) nano-encapsulation of chelidonine, an active bioingredient of greater celandine (Chelidonium majus), enhances its ameliorative potential against cadmium induced oxidative stress and hepatic injury in mice. Environ Toxicol Pharmacol 2013; 36(3): 937-947.

Freitag AF, Cardia GFE, da Rocha PR, et al. Hepatoprotective effect of Silymarin (Silybum marianum) on hepatotoxicity induced by acetaminophen in spontaneousely hypertensive rats. Evid based Compl Alternative Med 2015; 1-9.

Lovelace ES, Waqoner J, MacDonald T, et al. Silymarin suppresses cellular inflammation by inducing reparative stress signaling. J Nat Prod 2015; 78(8): 1990-2000.

Lian N, Jiang F, Jin H, et al. Curcumin regulates cell fate and metabolism by inhibiting hedgehog signaling in hepatic stellate cells. Lab Investig 2015; 95(7): 790-803.

ChrubasikS, Endelein W, Bauer R, Grabner W. Evidence for antirheumatic effectivness of. stewed Herba utricae dioicae in acute arthritis: a pilot study. Phytomedicine 1997; 4: 105-108.

Halboos MHN. Spectrophotometric Method for Determination and Biological Activity of Trace Amount of Cd(II) by bis(2-((pyridin-2-ylimino)methyl)phenyl)-4,4'- (diazene-1,2-diyl)dibenzoate. Int J Sci Res 2015; 4(3): 2222-2226.

Guan S, Peng C, Li H, Xu S, Yuan Z. Determination of biological activity of extract from hirudo by N-benzoyl-L-arginine ethyl ester. Zhongguo Zhong Yao Za Zhi 2010; 35(15): 1990-1992.

Torii Y, Goto Y, Takahashi M, Ishida S, Harakawa T, Sakamoto T, et al. Quantitative determination of biological activity of botulinum toxins utilizing compound muscle action potentials (CMAP), and comparison of neuromuscular transmission blockage and muscle flaccidity among toxins. Toxicon 2009; 55(2-3): 407-14.

Zhang H, Zhang ?, Xu B. Analysis and determination of biological activity of short-chain peptides from porcine brain hydrolysate. J Pharm Biomed Anal 2005; 37(2): 333-9.

Katafuchi T, Hamano K, Minamino N. Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides. Biochem Biophys Res Commun 2004; 313(1): 74-9.

Penkov D, Dimitrovs S, Andronova V, Milieva E, Murdjeva M, Stanimirova I, et al. Biological activity of bulgarian Folia Betulae dry extract. Int J Pharm Pharmaceut Sci 2015; 7(7): 124-136.

Luesch H, Yoshida WY, Moore RE, Paul VJ, Moorberry SL. Isolation, structure determination, and biological activity of Lyngbyabellin A from the marine cyanobacterium lyngbya majuscula. J Nat Prod 2000; 63(5): 611-5.

Schulte M, Brecht-Krauss D, Heymer B, Guhlmann A, Hartwig E, Sarkar MR., Schulte M, Brecht-Krauss D, Heymer B, et al. Fluorodeoxyglucose positron emission tomography of soft tissue tumours: is a non-invasive determination of biological activity possible. Eur J Nucl Med 1999; 26(6): 599-605.

Chopin V, Matias I, Stefano GB, Salzet M. Amino acid sequence determination and biological activity of therin, a naturally occuring specific trypsin inhibitor from the leech Theromyzon tessulatum. Eur J Biochem 1998; 254(3): 565-70.

Takei Y, Takano M, Itahara Y, Watanabe TX, Nakajima K, Conkin DJ, et al. Rainbow Trout Ventricular Natriuretic Peptide: Isolation, Sequencing, and Determination of Biological Activity. Gen Comp Endocrinol 1995; 96(3): 420-6.

Fumiere M, Fumiere M, Poltronieri H, Endringe R. Seasonality modifies rosemary’s composition and biological activity. Ind Crop Prod 2015; 70: 106-118.

Moreta C, Tena M-T, Kannan K. Analytical method for the determination and a survey of parabens and their derivatives in pharmaceuticals. Environ Res 2015; 142: 452-460.

Preuett B, Leeder S, Abdel-Rahman S. Development and application of a high-throughput screening method to evaluate antifungal activity against trichophyton tonsurans. J Biomol Screen 2015.

Chakraborty M, Karmakar I, Haldar S, Nepal A, Haldar P. Anticancer and antioxidant activity of methanol extract of Hippophae Salicifolia in EAC induced swiss albino mice. Int J Pharm Pharmaceut Sci 2015; 7(8): 24-32.

Pincus SM, Keefe DL. Quantification of hormone pulsatility via approximate entropy algorithm. Am J Physiol 1992; 262: 741-754.

Pincus SM, Viscarello RR. Approximate entropy: a regularity measure for fetal heart rate analysis. Obstet Gynecol 1992; 79 (2): 249-255.

Veldhius JD, Roelfsema F, Iranmanesh A, Carroll B, Keenan D, Pincus S. Basal, Pulsatile, Entropic (Patterned), and Spiky (Staccato-like) Properties of ACTH Secretion: Impact of Age, Gender, and Body Mass Index. J Clin Endocrinol Metab 2009; 94: 4045-4052.

Tejera E, Plain A, Portelinha A, Caceres J, Rebelo I, Nieto-Villar J. Heart rate variability complexity in the aging process. J Comp Math Meth Med 2007; 18(4): 287-296.

Safara F, Doraisamy S, Azman A, Jantan A, Ranga S. Wavelet Packet Entropy for Heart Murmurs Classification. Adv Bioinformatics 2012; 4.

Darbin O, Dees D, Martino A, Adams E, Nariyoku D. An entropy-based model for basal ganglia dysfunctions in movement disorders. Biomed Res Int 2013; 2013: 5.

Wu, H-T, Lee C-Y, Liu A-B. Multiscale cross-approximate entropy analysis as a measurement of complexity between ecg r-r interval and ppg pulse amplitude series among the normal and diabetic subjects. Comput Math Methods Med 2013; 2013.

Molnar J, Thornton B, Molnar A, Gaal D, Luo L, Bergmann-Leitner E. Thermodynamic aspects of cancer: possible role of negative entropy in tumor growth, its relation to kinetic and genetic resistance. Lett Drug Des Discov 2005; 2: 429-438.

Luo L F, Molnar J, Dong H, Lv XG, Spengler G. Attempts to introduce thermodynamics in anticancer therapy. Acta Sci Nat Univ Intramongol 2006; 36: 295-303.

Luo L F, Molnar J, Dong H, Lv XG, Spengler G. Physicochemical attack against solid tumors based on the reversal of direction of entropy flow: an attempt to introduce thermodynamics in anticancer therapy. Diagn Pathol 2006; 1(43).

Kayser K, Borkenfeld S, Goldman T, Kayser G. How to measure diagnosis-associated information in virtual slides. Diagn Pathol 2010; 6(11). http://www.diagnosticpathology. org/content/6/S1/S9

Kayser K, Borkenfeld S, Goldman T, Kayser G. To be at the right place at the right time. Diagn Pathol 2011; 6(68):

Kayser K, Gabius HJ. The application of thermodynamic principles to histochemical and morphometric tissue research: principles and practical outline with focus on the glycosciences. Cell Tissue Res 1999; 3: 443-455.

Kayser K. Qantitive pathology in virtual microscopy: History, applications, perspectives. Acta Histochem 2013; 12: 179-188.

Dunn J, Hveem T, Pretorius M, Oukrif D, Nielsen B, Albergsten L, et al. Comparison of nuclear texture analysis and image cytometric DNA analysis for the assessment of dysplasia in Barrett's oesophagus. Br J Canc 2011; 105(8): 1218-1223.

Vido J, Adam, R, Lorand-Metze K, Metze K. Computerized texture analysis of atypical immature myeloid precursors in patients with myelodysplastic syndromes: an entity between blasts and promyelocytes. Diagn Pathol 2011; 6(93).

Areshidze D, Timchenko L, Klimenko A, Gulyukin M, Kozlova M. Influence of an Enzymatic Hydrolyzate of Chlorophytum comosum (L.) on Morphofunctional Integrity of a Liver of White Rats at ExperimentalToxic Damage During Various Periods of Ontogenesis. Global Veterinaria; 2013; 11(6): 794-802.

Areshidze D, Timchenko L, Kozlova M. Information Condition of the Liver of Dogs at Pathologies at the Reproductive Period of Ontogenesis. Academic Journal of Cancer Research 2013; 6(2): 79-83.

Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol 2003; 33(2): 105-136.

Boll M, Weber L, Becker E, Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z Naturforsch C 2001; 56(7-8): 649-659.

Dalton SR, Lee SML, King RN, et al. Carbon tetrachloride-induced liver damage in asialoglycoprotein receptor-deficient mice. Biochem Pharmacol 2009; 77: 1283-1290.

Avtandilov G, Barsukov V. Informational analysis of immune and endocrine organs. Morphological changes in the course of infection. Zentralbl Pathol 1992; 138(5): 345-349.

Areshidze D, Timchenko L, Kozlova M. Information condition of the liver of dogs at pathologies at the reproductive period of ontogenesis. Acad J Canc Res 2013; 6(2): 79-83.




How to Cite

David Areshidze, Lyudmila Timchenko, Maria Kozlova, Igor Rzhepakovsky, & Ivan Syomin. (2016). Use of Information Parameters as Criterion for Determination of Biological Activity of Hepatoprotective Preparations. Journal of Pharmacy and Nutrition Sciences, 6(2), 61–71.