Background: The human organism is a complex superorganism including numerous eukaryotic, eubacterial, and archaean cells. The qualitative and quantitative assessment of the microbiota toxicity of chemical agents, i.e., their inhibitory effects on the microbial inhabitants of the human organism in health and disease, seems to hold much value in this context. In this work, a bacterial luminescence-based express test system for microbiota toxicity is applied to neurotransmitters such as serotonin, dopamine, norepinephrine, and histamine.
Methods: The biosensor was based on a GM Escherichia coli K12 strain (TGI) that contained the lux operon of the luminescent soil bacterium Photorhabdus luminescencens ZMI. The biosensor was exposed to the action of the tested neurotransmitters for 5 to 60 minutes The intensity of bacterial luminescence (counts.sec-1) was monitored in the control and the experimental samples with a Biotoks 6 ms luminometer (Russia); the toxicity index (T) of the neurotransmitters was determined.
Results: A marked toxic effect on bioluminescence was produced by serotonin, histamine, and dopamine at concentrations exceeding 80 µg/ml, 100 µg/ml, and 1 mg/ml, respectively. At lower concentration, these neurotransmitters were “negatively toxic”, i.e. stimulatory in terms of the effect on bacterial luminescence. In contrast, norepinephrine inhibited luminescence at all concentrations tested.
Conclusions: The bacterial luminescence-based testing method is applicable to the assessment of the destructive and stimulatory effects of neurotransmitters; the data obtained are of microbiological and clinical relevance.
Shenderov BA. Microbial ecology and its role in promoting health. Metamorfozy (Russian) 2014; 5: 72-80.
El Aidy S, Dinan TG, Cryan JF. Gut microbiota: the conductor in the orchestra of immune-neuroendocrine communication. Clin Ther 2015; 37: 954-67. https://doi.org/10.1016/j.clinthera.2015.03.002
Krishnan S, Alden N, Lee K. Pathways and functions of gut metabolism impacting host physiology. Curr Opin Biotech 2015; 36: 137-45. https://doi.org/10.1016/j.copbio.2015.08.015
Zarubina AP, Sorokina EV. First among equals. The bacterial luminescence test: one of the quickest and easiest bioassays. J Eurasian Researcher Union Biol Ser (Russian) 2015; 17(8): 161-3.
Zarubina AP, Perfiliev YD, Sorokina EV, et al. Evaluation of the properties of potassium ferrate used for water purification by luminescence bioassay. Mosc Univ Biol Bull 2016; 71(4): 226-30. https://doi.org/10.3103/S0096392516040131
Zarubina AP, Gapochka MG, Novoselova LA, et al. Effect of low intensity electromagnetic radiation on the toxicity of domestic wastewater tested with the «ecolum» test system. Mosc Univ Biol Bull 2013; 68(1): 49-52. https://doi.org/10.3103/S0096392512030108
Bulich AA, Tung KK, Scheibner G. The luminescent bacteria toxicity test: its potential use as an in vitro alternative. Biolum Chemilum 1990; 5(2): 71-77. https://doi.org/10.1002/bio.1170050202
Zarubina AP, Deev LI, Parkhomenko IM, et al. Evaluation of toxicity of argentum ions and nanoparticles on the model bacterial object with luminescent phenotype. Nan Sci Rus 2015; 10(5-6): 475-83.
Kaiser KL. Correlation of Vibrio fischeri bacteria test data with bioassay data for other organisms. //Environ Health Persp 1998; 106(2): 583-91. https://doi.org/10.1289/ehp.98106583
Ashmarin IP, Eshchenko ND, Karazeeva EP Neurochemistry in tables and diagrams. Moscow: Exam 2007.
Vasiliev VN Diagnosis and treatment of incurable nervous and mental diseases of dopamine etiology. Moscow: Media Kit; 2009.
Oleskin AV, El’-Registan GI, Shenderov BA. Role of neuromediators in the functioning of the human microbiota: «business talks» among microorganisms and the microbiota-host dialogue. Microbiology 2016; 85(1): 1-22. https://doi.org/10.1134/S0026261716010082
Anuchin AM, Chuvelev DI, Kirovskaya TA, et al. Effect of neuromediator monoamines on the growth characteristics of Escherichia coli K-12. Microbiology 2008; 77(6): 758-765. https://doi.org/10.1134/S0026261708060040
Oleskin AV, Kirovskaya TA, Botvinko IV, et al. Effect of serotonin (5-hydroxytryptamine) on the growth and differentiation of microorganisms. Microbiology 1998; 67(3): 306-31.
Danilov VS, Zarubina AP, Eroshnicov GE, et al. The biolumiscent sensor systems with lux operons from various species of luminescent bacteria. Mosc Univ Biol Bull 2002; 57(3): 20-4.
De Backer D, Biston P, Devriendt J, et al. Comparison of dopamine and norepinephrine in the treatment of shock. New Engl J Med 2010; 362(9): 779-89. https://doi.org/10.1056/NEJMoa0907118
Inoue W, Baimoukhametova D.V, Füzesi T, et al. Noradrenaline is a stress-associated metaplastic signal at GABA synapses. Nat Neurosci 2013; 16(5): 605-12. https://doi.org/10.1038/nn.3373
Oleskin AV, Shishov VI, Malikina KD. Symbiotic biofilms and brain neurochemistry. Hauppage (NY): Nova Science Publishers 2010.
Malikina KD, Shishov VA, Chuvelev DI, et al. Regulatory role of neuromediator amines in Saccharomyces cerevisiae cells. Appl Biochem Micro 2010; 46(6): 672-7. https://doi.org/10.1134/S0003683810060104
Xue R, Zhang YP, Jin ZL, et al. The discovery of 071031B, a novel serotonin and noradrenaline reuptake inhibitor. Neurosci Lett 2013; 544(1): 68-73. https://doi.org/10.1016/j.neulet.2013.02.076
Isbister GK, Bowe SJ, Dawson A, et al. Relative toxicity of selective serotonin reuptake inhibitors (SSRIs) in overdose. J Toxicol-Clin Toxic 2004; 42(3): 277-85. https://doi.org/10.1081/CLT-120037428
Sarkar C, Basu B, Chakroborty D, et al. The immunoregulatory role of dopamine: an update. Brain Behav Immun 2010; 24(4): 525-8. https://doi.org/10.1016/j.bbi.2009.10.015
Sconbaume E, Sellers EA, Johnson GE. Noradrenaline and survival of rats in a cold environment. Can J Biochem Phys 1963; 41: 975-83. https://doi.org/10.1139/o63-111
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Copyright (c) 2017 Alexander V. Oleskin , Elena V. Sorokina , Alevtina P. Zarubina , Inna M. Parkhomenko