Abstract
Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either in natural or synthetic form but they are quite expensive and some of it not available in the market anymore. These problems strongly associated with the accuracy and reliability of High Performance Liquid Chromatography (HPLC) analysis data. Therefore, this study aimed to set up an analytical scheme of obtaining β-carotene standard from the leaves of Morinda citrifolia as one of the carotenoid standards for HPLC analysis. M. citrifolia has been selected due to its abundance throughout the year with tropical climate. The scheme via open column chromatography (OCC) established that the purity of β-carotene standard was 97% and the coefficient of correlation was 0.9923. However after 30 day storage period of time, the purity decreased to 95.46%. Although these had an effect on the carotenoid standard stability but it can be a reliable source of β-carotene standard for HPLC analysis as well as active pharmaceutical ingredient for cosmeceutical, nutraceutical, food and beverage industries.
References
Johnson EA, Schroeder WA. Advances in Biochemical Engineering and Biotechnology. Springer, Berlin 1996.
Othman R. Biochemistry and genetics of carotenoid composition in potato tubers. Lincoln University, Christchurch, New Zealand, PhD thesis 2009.
Britton G. Carotenoids Chemistry and Biology. Springer, New York 1989.
Norshazila S, Irwandi J, Rashidi O, Yumi Zuhanis HH. Scheme of obtaining ?-carotene standard from pumpkin (Cucurbita moschata) flesh. Int Food Res J 2012; 19: 531-535.
Fatimah AMZ, Norazian MH, Rashidi O. Identification of carotenoid composition in selected ‘ulam’ or traditional vegetables of Malaysia. Int Food Res J 2012; 19: 527-530.
Rodriguez-Amaya D, Kimura M. HarvestPlus handbook for carotenoid analysis. HarvestPlus, Washington DC 2004.
Saidin I. Sayuran tradisional, ulam dan penyedap rasa. Penerbit Universiti Kebangsaan Malaysia, Bangi, Malaysia 2000.
Tee ES, Lim CL. Carotenoids composition and content of Malaysian vegetables and fruits by the AOAC and HPLC methods. Food Chem 1991; 41: 309-339. https://doi.org/10.1016/0308-8146(91)90057-U
Rouchaud J, Moons C, Meyer JA. Effects of pesticide treatments on the carotenoid pigments of lettuce. J Agric Food Chem 1984; 32: 1241-1245. https://doi.org/10.1021/jf00126a008
Lee JJ, Crosby KM, Pike LM, Yoo KS, Leskovar DI. Impact of genetic and environmental variation on development of flavonoids and carotenoids in pepper (Capsicum spp.). Sci Hort 2005; 106: 341-352. https://doi.org/10.1016/j.scienta.2005.04.008
Schreiner M, Huyskens-Keil S. Phytochemicals in fruit and vegetables: Health promotion and postharvest elicitors. Crit Rev in Plant Sci 2006; 25: 267-278. https://doi.org/10.1080/07352680600671661
Solovchenko AE, Avertcheva OV, Merzlyak MN. Elevated sunlight promotes ripening-associated pigment changes in apple fruit. Postharvest Biol Technol 2006; 40: 183-189. https://doi.org/10.1016/j.postharvbio.2006.01.013
Lefsrud M, Kopsell D, Wenzel A, Sheehan J. Changes in kale (Brassica oleracea L. var. acephala) carotenoid and chlorophyll pigment concentrations during leaf ontogeny. Sci Hort 2007; 112: 136-141. https://doi.org/10.1016/j.scienta.2006.12.026
Aherne SA, Jiwan MA, Daly T, O’Brien NM. Geographical location has greater impact on carotenoid content and bioaccessibility from tomatoes than variety. Plant Foods Hum Nutr 2009; 64: 250-256. https://doi.org/10.1007/s11130-009-0136-x
Xu JG, Hu QP, Wang XD, Luo JY, Liu Y, Tian CR. Changes in the main nutrients, phytochemicals, and antioxidant activity in yellow corn grain during maturation. J Agric Food Chem 2010; 58: 5751-5756. https://doi.org/10.1021/jf100364k
Yang J, Zhu Z, Wang Z, Zhu B. Effects of storage temperature on the contents of carotenoids and glucosinolates in Pakchoi (Brassica rapa L. ssp. Chinensis Var. Communis). J Food Biochem 2010; 34: 1186-1204. https://doi.org/10.1111/j.1745-4514.2010.00358.x
Adewusi SRA, Bradbury JH. Carotenoids in cassava: Comparison of open-column and HPLC methods of analysis. J Sci Food Agr 1993; 62: 375-383. https://doi.org/10.1002/jsfa.2740620411
Carvalho PRN, Collins CH, Rodriguez-Amaya DB. Comparison of provitaminA determination by normal-phase gravity- flow column chromatography and reversed-phase high performance liquid chromatography. Chromatographia 1993; 33: 133-137. https://doi.org/10.1007/BF02275893
Kimura M. Rodriguez-Amaya D. A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoids. Food Chem 2002; 78: 389-398. https://doi.org/10.1016/S0308-8146(02)00203-0
Britton G. Structure and properties of carotenoids in relation to function. FASEB J 1995; 9: 1551-1558.
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Copyright (c) 2017 Rashidi Othman , Fatimah Azzahra Mohd Zaifuddin , Norazian Mohd Hassan