Carotenoid Content and Composition in 20 Medicinal Plant Species of Traditional Malay Midwifery Postnatal Bath


 Malay midwifery, postnatal traditional bath, carotenoids, antioxidant, active pharmaceutical ingredients, natural bioactive compounds.

How to Cite

Rashidi Othman, Siti Farah Aliya Abdul Halim, Farah Ayuni Mohd Hatta, & Mohd Aizat Jamaludin. (2017). Carotenoid Content and Composition in 20 Medicinal Plant Species of Traditional Malay Midwifery Postnatal Bath. Journal of Pharmacy and Nutrition Sciences, 7(4), 193–197.


Today in Malay community, midwifery traditional knowledge of herbal medicine has disappeared and extinct. The facts are Malay midwives are becoming rare and the more crucial is medicinal plants are over-harvested. The aim of this research is to identify and investigate the active pharmaceutical ingredients content in 20 selected species used in the Malay traditional bath. There is a solid need to analyse the potential of these natural bioactive compounds, particularly carotenoids to be fully utilised and commercialised especially in halal market and health advantages. Through High performance liquid chromatography (HPLC) analysis, all 20 species were found to have at least four individual carotenoid pigments with a relatively high concentration of lutein and β-carotene and lower concentrations of zeaxanthin. Strobilanthes crispus (Pecah Kaca) leaf was detected to have the highest total carotenoid content (1546.80±283.45 μg/g DW)while Psidium guajava (Jambu Batu) shoot has the lowest total carotenoid content (112.9±82.2 μg/g DW). The significant outcome of the research was a new findings of new natural bioactive compound sources as health promoting agents which covers not only the Shariah requirement, but also safety aspects. Moreover, it will preserve the traditional knowledge of Malay traditional bath practices.


Skeat WW. Malay Magic - an introduction to the folklore and popular religion of the Malay Peninsula. Macmillan and Co., Limited, UK, 1900.

Ali A, Howden-Chapman, P. Maternity services and the role of the traditional birth attendant, bidan kampung, in rural Malaysia. J Public Health Manag Pract 2007; 13(3): 278-286.

Karim WJ. Malay midwives and witches. Soc Sci Med 1984; 18(2): 159-166.

Hasan Z, Mazlan NA, Yaacob, M. Tumbuhan ubatan untuk kesejahteraan wanita. Institut Penyelidikan dan Kemajuan Pertanian Malaysia (MARDI) dan NRH Publications Sdn. Bhd., Malaysia 2005.

Ramli AG, Shamsaini S, Jaafar L, Fariza, F. Traditional and Complementary Medicine Practice Guideline on Malay Massage. Traditional and Complementary Medicine Division, Ministry of Health, Malaysia 2009.

Ajayi I, Ajibade O, Oderinde, R. Preliminary phytochemical analysis of some plant seeds. Res J Chem Sci 2011; 1(3): 3-7.

Sati SC, Sati N, Rawat U, Sati, OP. Medicinal plants as a source of antioxidants. Res J Phytochem 2010; 4(4): 213-224.

Othman R, Kammona S, Jaswir I, Jamal, P. Characterisation of carotenoid content in diverse local sweet potato (Ipomoea batatas) flesh tubers. Int J Pharm Pharm Sci 2015; 7(2): 347-351.

Rodriguez-Amaya DB. Latin American food sources of carotenoids. Arch Latinoam Nutr, 1999; 49: 74S-84S.

Sangeetha RK, Baskaran, V. Carotenoid composition and retinol equivalent in plants of nutritional and medicinal importance: Efficacy of? ?-carotene from Chenopodium album in retinol-deficient rats. Food Chem 2010; 119(4):1584-1590.

Othman R. Biochemistry and genetics of carotenoid composition in potato tubers. 2009; Ph.D. Dissertation. Lincoln University, New Zealand.

Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol, 1994; 144: 301-313.

Fatimah MZ, Norazian MH, Rashidi, O. Identification of carotenoid composition in selected “ulam” or traditional vegetables in Malaysia. Int Food Res J 2012; 19(2): 527-530.

Wong JY, Matanjun P, Ooi YBH, Chia, KF. Characterization of phenolic compounds, carotenoids, vitamins and antioxidant activities of selected Malaysian wild edible plants. Int J Food Sci Nutr 2013; 64: 1-11.

Znidarcic D, Ban D, Sircelj, H. Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. Food Chem 2011; 129: 1164-1168.

Gutierrez RMP, Mitchell S, Solis, RV. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 2008; 117(1): 1-27.

Hirschberg J. Production of high-value compounds: carotenoids and vitamin E. Curr Opin Biotechnol 1999; 10:186-191.

Cunningham FX, Gantt, E. Genes and enzymes of carotenoid biosynthesis in plants. Ann Rev Plant Physiol Plant Mol Biol 1998; 49: 557-583.

Rodriguez-Amaya DB. Important Food Sources of Provitamins A. Carotenoids and food preparation: The retention of provitamin A carotenoids in prepared, processed, and stored foods. OMNI Press, Washington, 1997.

Khoo HE, Prasad KN, Kong KW, Jiang Y, Ismail, A. Carotenoids and their isomers: Color pigments in fruits and vegetables. Molecules 2011; 16(2): 1710-1738.

Morosinotto T, Caffarri S, Dall’Osto L, Bassi, R. Mechanistic aspects of the xanthophyll dynamics in higher plant thylakoids. Physiol Plant 2003; 119: 347-354.

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Copyright (c) 2017 Rashidi Othman, Siti Farah Aliya Abdul Halim, Farah Ayuni Mohd Hatta , Mohd Aizat Jamaludin