Antioxidant Activity and other Physicochemical Characteristics of Different Propolis Extracts


Propolis, antioxidant, Liquid Chromatography-Mass Spectrometry, Gas Chromatography-Mass Spectrometry, Folin-Ciocalteu.

How to Cite

Alaa Zam, & Mohammad Othman. (2018). Antioxidant Activity and other Physicochemical Characteristics of Different Propolis Extracts. Journal of Pharmacy and Nutrition Sciences, 8(2), 59–67.


This study was to investigate the antioxidant activity of three different extracts of propolis (Ethanolic 50%, methanolic 100% and aqueous extracts) with some of their physicochemical aspects. Ethanolic extract was analyzed by LC-MS, while the methanolic extract was analyzed by GC-MS in order to screen polyphenols and volatile compounds in the sample, the identity of these components were achieved by means of comparing their mass spectra with previous published studies. Forrier transformation infrared spectroscopy (FTIR) was conducted on the methanolic extract and crude propolis samples to gain an overview of some physical characteristics. The antioxidant activities of the three extracts were measured by DPPH scavenging ability and FRAP assay. LC-MS and GC-MS allowed the identification of 19 phenolic and 17 volatile compounds. The most abundant phenolics found in our sample correspond with p-Coumaric benzyl ester, Pinobanksin-3-O-acetate, Caffeic acid benzyl ester and pinocembrin, as they showed the highest peaks in the total ion chromatogram (TIC). In addition, the most abundant volatile compounds showed a high level of agreement with δ-Cadinene and γ- Terpinen.Furthermore, Ethanol 50% extract showed the strongest antioxidant activity, which has a significant correlation with its high polyphenolic content.


Volpi N, Bergonzini G. Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry. J Pharm Biomed Anal 2006; 354: 361-3.

Miguel MG, Antunes MD. Is propolis safe as an alternative medicine? J Pharm Bioall Sci 2011; 479:495-4.

Toreti VC, et al. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evid Based Complement Alternat Med 2013; 1: 13-2013.

Gulcin I, et al. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol 2010; 2227: 2238-8.

Huang S, et al. Recent advances in the chemical composition of propolis. Molecules 2014; 19610: 19632-12.

Silva JC, et al. Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food Chem Toxicol 2012; 1790: 1795-5.

Gardana C, et al. Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal 2007; 390: 399-3.

Ramos AFN, Miranda JL. Propolis: a review of its anti-inflammatory and healing actions. J Venom Anim Toxin Incl Trop Dis 2007; 697: 710-4.

Pellati F, Prencipe FP, Benvenuti S. Headspace solid-phase microextraction-gas chromatography-mass spectrometry characterization of propolis volatile compounds. J Pharm Biomed Anal 2013; 103: 111-84.

Russo A, Longo R, Vanella A. Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia 2002; S21: 29-73.

Nakajima Y, et al. Water extract of propolis and its main constituents, caffeoylquinic acid derivatives, exert neuroprotective effects via antioxidant actions. Life Sci 2007; 370: 377-4.

Sun C, et al. Effect of Ethanol/Water Solvents on Phenolic Profiles and Antioxidant Properties of Beijing Propolis Extracts. Evid Based Complement Alternat Med 2015; 1: 9-2015.

Penton ZE. Headspace gas chromatography. In: J. Pawliszyn, editors. Comprehensive Analytical Chemistry. Elsevier Science 2002; pp. 279-296.

Ötles S. Analysis of Polyphenols in Foods. In: Ötles S, editors. Methods of analysis of food components and additives. 2nd ed. Boca Raton: CRC Press, 2012; pp. 199-260.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 1841: 1856-6.

Li S, et al. Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Ind Crops Prod 2013; 289: 298-51.

Falcao SI, et al. Phenolic profiling of Portuguese propolis by LC-MS spectrometry: uncommon propolis rich in flavonoid glycosides. Phytochem Anal 2013; 309: 318-4.

Banskota AH, Tezuka Y, Kadota S. Recent progress in pharmacological research of propolis. Phytother Res 2001; 561: 571-7.

Dias LG, Pereira AP, Estevinho LM. Comparative study of different Portuguese samples of propolis: pollinic, sensorial, physicochemical, microbiological characterization and antibacterial activity. Food Chem Toxicol 2012; 4246: 4253-12.

Falcao SI, et al. Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds. Anal Bioanal Chem 2010; 887:897-2.

Pellati F, et al. HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. J Pharm Biomed Anal 2011; 934: 948-5.

Nunes CA, Guerreiro MC. Characterization of Brazilian green propolis throughout the seasons by headspace GC/MS and ESI-MS. J Sci Food Agric 2012; 433: 438-2.

Bridi R, et al. International Regulations of Propolis Quality: Required Assays do not Necessarily Reflect their Polyphenolic-Related In Vitro Activities. J Food Sci 2015; C1188: 1195-6.

Vinson JA, et al. Plant Flavonoids, Especially Tea Flavonols, Are Powerful Antioxidants Using an in Vitro Oxidation Model for Heart Disease. J Agric Food Chem 1995; 2800: 2802-11.

Debbache N, Atmani D, Atmani D. Chemical analysis and biological activities of Populus nigra, flower buds extracts as source of propolis in Algeria. Ind Crops Prod 2014; 85: 92-53.

Mohammad Zadeh S, et al. Antioxidant power of Iranian propolis extract. Food Chemistry 2007; 729: 733-3.

Silva V, et al. Antioxidant activity of uruguayan propolis. In vitro and cellular assays. J Agric Food Chem 2011; 6430: 6437-12.

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