Azithromycin-Chitosan Films for Improved Haemostasis and Wound Healing


  • Linda Fateh Damascus University, Syria
  • Susan Waiss   Scientific Studies and Research Centre, Damascus, Syria
  • Mohammad Othman Damascus University, Syria



Azithromycin AZM, Chitosan CS, wound healing, haemostatic, film.


Wound healing is a global medical concern, because haemorrhage is still a major cause of morbidity and mortality. Therefore, more effective and cost-effective dressings are required. Antimicrobial dressing of Azithromycin (AZM) (a broad-spectrum macrolide antibiotic) with Chitosan (CS) as a carrier, has a lot of desired properties such as haemostatic, pain-reducing, and less scar forming. It is prepared by a solvent evaporation method. The film morphology is investigated by SEM and film-water wettability is measured by a contact angle method. The drug-polymer interactions are evaluated by using FTIR and DSC. Azithromycin loaded films (different mass ratio of AZM to CS w % 10%, 25% and 50%) were sterilized by Gamma radiation, then their antimicrobial characteristics and haemostatic performance were evaluated. Film thickness was about 9 µm. The wettability properties of films were enhanced by adding AZM and PEG6000 5% (mass ratio of PEG 6000 to CS w %). Microbial growth inhibitions were identified for films loaded with Azithromycin.


Di Lena F. Hemostatic polymers: The concept, state of the art and perspectives [Internet

Yu L, Shang X, Chen H, Xiao L, Zhu Y, Fan J. A tightly-bonded and flexible mesoporous zeolite-cotton hybrid hemostat. Nat Commun [Internet

Khoshmohabat H, Paydar S, Kazemi HM, Dalfardi B. Overview of agents used for emergency hemostasis. Trauma Monthly. Kowsar Medical Publishing Company 2016; Vol. 21. DOI:

Groenewold MD, Gribnau AJ, Ubbink DT. Topical haemostatic agents for skin wounds: A systematic review. BMC Surg [Internet

Nakielski P, Pierini F. Blood interactions with nano- and microfibers: Recent advances, challenges and applications in nano- and microfibrous hemostatic agents [Internet

Boateng J, Catanzano O. Advanced Therapeutic Dressings for Effective Wound Healing - A Review. J Pharm Sci [Internet

Hamdan S, Pastar I, Drakulich S, Dikici E, Tomic-Canic M, Deo S, et al. Nanotechnology-Driven Therapeutic Interventions in Wound Healing: Potential Uses and Applications. ACS Cent Sci 2017; 3(3): 163-75. DOI:

Percival SL, McCarty S, Hunt JA, Woods EJ. The effects of pH on wound healing, biofilms, and antimicrobial efficacy [Internet

Dai T, Tanaka M, Huang YY, Hamblin MR. Chitosan preparations for wounds and burns: Antimicrobial and wound-healing effects [Internet

Halstead FD, Rauf M, Bamford A, Wearn CM, Bishop JRB, Burt R, et al. Antimicrobial dressings: Comparison of the ability of a panel of dressings to prevent biofilm formation by key burn wound pathogens. Burns [Internet

Rukavina Z, Šegvi? Klari? M, Filipovi?-Gr?i? J, Lovri? J, Vani? Ž. Azithromycin-loaded liposomes for enhanced topical treatment of methicillin-resistant Staphyloccocus aureus (MRSA) infections. Int J Pharm [Internet

Ghosal K, Chandra A, Praveen G, Snigdha S, Roy S, Agatemor C, et al. Electrospinning over Solvent Casting: Tuning of Mechanical Properties of Membranes. Sci Rep [Internet

Hassan MA, Omer AM, Abbas E, Baset WMA, Tamer TM. Preparation, physicochemical characterization and antimicrobial activities of novel two phenolic chitosan Schiff base derivatives. Sci Rep 2018; 8(1): 1-14. DOI:

Xu F, Weng B, Materon LA, Gilkerson R, Lozano K. Large-scale production of a ternary composite nanofiber membrane for wound dressing applications. J Bioact Compat Polym [Internet

Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering [Internet

Pilehvar-Soltanahmadi Y, Akbarzadeh A, Moazzez-Lalaklo N, Zarghami N. An update on clinical applications of electrospun nanofibers for skin bioengineering [Internet

Gu BK, Park SJ, Kim MS, Kang CM, Kim J Il, Kim CH. Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials. Carbohydr Polym [Internet

Gandhi R, Pillai O, Thilagavathi R, Gopalakrishnan B, Kaul CL, Panchagnula R. Characterization of Azithromycin hydrates. Eur J Pharm Sci [Internet

McMullan BJ, Mostaghim M. Prescribing azithromycin. Aust Prescr [Internet

Payab S, Jafari-Aghdam N, Barzegar-Jalali M, Mohammadi G, Lotfipour F, Gholikhani T, et al. Preparation and physicochemical characterization of the azithromycin-Eudragit RS 100 nanobeads and nanofibers using electrospinning method. J Drug Deliv Sci Technol [Internet

Azhdarzadeh M, Lotfipour F, Zakeri-Milani P, Mohammadi G, Valizadeh H. Anti-bacterial performance of azithromycin nanoparticles as colloidal drug delivery system against different gram-negative and gram-positive bacteria. Adv Pharm Bull 2012; 2(1): 17-24.

Liang D, Lu Z, Yang H, Gao J, Chen R. Novel Asymmetric Wettable AgNPs/Chitosan Wound Dressing: In vitro and in vivo Evaluation. ACS Appl Mater Interfaces. 2016 Feb 24; 8(6): 3958-68. DOI:

Mathew A, Vaquette C, Hashimi S, Rathnayake I, Huygens F, Hutmacher DW, et al. Antimicrobial and Immunomodulatory Surface-Functionalized Electrospun Membranes for Bone Regeneration. Adv Healthc Mater 2017; 6(10). DOI:

Kauss T, Gaubert A, Boyer C, Ba BB, Manse M, Massip S, et al. Pharmaceutical development and optimization of azithromycin suppository for paediatric use. Int J Pharm [Internet

Aucamp M, Odendaal R, Liebenberg W, Hamman J. Amorphous azithromycin with improved aqueous solubility and intestinal membrane permeability. Drug Dev Ind Pharm [Internet

Schreml S, Szeimies RM, Karrer S, Heinlin J, Landthaler M, Babilas P. The impact of the pH value on skin integrity and cutaneous wound healing. J Eur Acad Dermatology Venereol 2010; 24(4): 373-8. DOI:

Power G, Moore Z, O’Connor T. Measurement of pH, exudate composition and temperature in wound healing: A systematic review. J Wound Care [Internet

Shuai H-H, Yang C-Y, Harn HI-C, York RL, Liao T-C, Chen W-S, et al. Using surfaces to modulate the morphology and structure of attached cells – a case of cancer cells on chitosan membranes. Chem Sci [Internet

Habiba U, Afifi AM, Salleh A, Ang BC. Chitosan/(polyvinyl alcohol)/zeolite electrospun composite nanofibrous membrane for adsorption of Cr6+, Fe3+ and Ni2+. J Hazard Mater [Internet

Contact Angle [Internet

Sun X, Tang Z, Pan M, Wang Z, Yang H, Liu H. Chitosan/kaolin composite porous microspheres with high hemostatic efficacy. Carbohydr Polym [Internet

Fathi P, Sikorski M, Christodoulides K, Langan K, Choi YS, Titcomb M, et al. Zeolite-loaded alginate-chitosan hydrogel beads as a topical hemostat. J Biomed Mater Res - Part B Appl Biomater [Internet

Tan L, Hu J, Huang H, Han J, Hu H. Study of multi-functional electrospun composite nanofibrous mats for smart wound healing. Int J Biol Macromol [Internet

Gu BK, Park SJ, Kim MS, Lee YJ, Kim J-I, Kim C-H. Gelatin blending and sonication of chitosan nanofiber mats produce synergistic effects on hemostatic functions. Int J Biol Macromol [Internet

Madhumathi K, Sudheesh Kumar PT, Abhilash S, Sreeja V, Tamura H, Manzoor K, et al. Development of novel chitin/nanosilver composite scaffolds for wound dressing applications. J Mater Sci Mater Med [Internet

Quan K, Li G, Tao L, Xie Q, Yuan Q, Wang X. Diaminopropionic Acid Reinforced Graphene Sponge and Its Use for Hemostasis. ACS Appl Mater Interfaces [Internet

Ong SY, Wu J, Moochhala SM, Tan MH, Lu J. Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials [Internet

Ruini F, Tonda-Turo C, Chiono V, Ciardelli G. Chitosan membranes for tissue engineering: Comparison of different crosslinkers. Biomed Mater [Internet

Thein-Han WW, Saikhun J, Pholpramoo C, Misra RDK, Kitiyanant Y. Chitosan-gelatin scaffolds for tissue engineering: Physico-chemical properties and biological response of buffalo embryonic stem cells and transfectant of GFP-buffalo embryonic stem cells. Acta Biomater [Internet

Bonilla J, Fortunati E, Atarés L, Chiralt A, Kenny JM. Physical, structural and antimicrobial properties of poly vinyl alcohol–chitosan biodegradable films. Food Hydrocoll [Internet

Adeli E. Preparation and evaluation of azithromycin binary solid dispersions using various polyethylene glycols for the improvement of the drug solubility and dissolution rate. Brazilian J Pharm Sci [Internet

Li L, Deng J, Deng H, Liu Z, Li X. Preparation , characterization and antimicrobial activities of chitosan / Ag / ZnO blend films. Chem Eng J [Internet




How to Cite

Linda Fateh, SusanWaiss , & Mohammad Othman. (2019). Azithromycin-Chitosan Films for Improved Haemostasis and Wound Healing. Journal of Pharmacy and Nutrition Sciences, 9(6), 305–315.