Extraction, and Characterization of Carotenoids from 11 Allelopathic Plant Species as Potential Halal Food Colorants and Active Pharmaceutical Ingredients
DOI:
https://doi.org/10.29169/1927-5951.2020.10.01.4Keywords:
Allelopathic species, carotenoids, active pharmaceutical ingredients, grass, fern, herb, tree.Abstract
Carotenoids are a class of natural product compound that are currently being used as colouring agents and widely used in food industry. Carotenoids are bioactive pigments obtained mainly from plants through dietary intake. They possess good features in terms of dietary supplement, food colourant, and polymer stabiliser. The presence of 4 main carotenoids, which are β-carotene, zeaxanthin, lutein, and violaxanthin, were determined in 4 classes of allelopathic plant groups namely trees, ferns, grasses and herbaceous plants. This research aims to explore the carotenoid’s content and composition in 11 allelopathic species by HPLC analysis. A. auriculiformis (tree) was found to have the highest total carotenoid concentration (146.36 µg/g DW) that was substantially higher than all other species tested whereas the lowest total carotenoid concentration was found in S. palustris (fern) (3.76 µg/g DW). Lutein and β-carotene were detected highest in A. auriculiformis (tree), with 1024 ± 25.5 µg/g DW and 37.55 ± 3.16 µg/g DW, respectively. Violaxanthin and zeaxanthin were found substantially highest in M. cajuputi (tree) (5.02 ± 0.5 µg/g DW) and S. palustris (fern) (5.88 ± 0.19µg/g DW), respectively.
References
Mezzomo N, Ferreira SRS. Carotenoids functionality, sources, and processing by supercritical technology?: A review. J Chem 2016; 1-17. https://doi.org/10.1155/2016/3164312
Othman R, Noh NH, Mohd Hatta FA, Jamaludin MA. Natural Carotenoid Pigments of 6 Chlorophyta Freshwater Green Algae Species. J Pharm Nutr Sci 2018; 8: 1-5. https://doi.org/10.6000/1927-5951.2018.08.01.1
Rodriguez-Amaya DB, Rodriguez EB, Amaya-Farfan J. Advances in food carotenoid research: chemical and technological aspects, implications in human health. Ma1 J Nutr 2006; 12(1): 101-121.
Othman R, Mohd Zaifuddin FA, Mohd Hassan N. Characterisation of Carotenoid and Total Retinol Equivalent Content in Ulam and Medicinal Species as Alternative Food Intervention to Combat Vitamin A Deficiency. J Pharm Nutr Sci 2017; 7: 81-87. https://doi.org/10.6000/1927-5951.2017.07.03.2
Othman R, Mohd Hassan N, Abu Bakar AE, Noh NH, Ahmad Fadzillah N, Mahmad N. Manipulation of Environmental Stress Towards Lutein Production in Chlorella fusca Cell Culture. J Pharm Nutr Sci 2019; 9: 251-257.
Othman R, Md Amin NA, Abu Bakar AE, Ahmad Fadzillah N, Mahmad N. Carotenoid Pigments of Red, Green and Brown Macroalgae Species as Potential Active Pharmaceutical Ingredients. J Pharm Nutr Sci 2019; 9: 14-19.
Othman R, Mohd Zaifuddin FA, Mohd Hassan N. Isolation and Purification of ? -Carotene from Morinda citrifolia as HPLC Standard and Active Pharmaceutical Ingredient. J Pharm Nutr Sci 2017; 7: 95-99. https://doi.org/10.6000/1927-5951.2017.07.03.4
Santos DT, Albuquerque CLC, Meireles MAA. Antioxidant dye and pigment extraction using a homemade pressurized solvent extraction system. Procedia Food Sci 2011; 1: 1581-1588. https://doi.org/10.1016/j.profoo.2011.09.234
Arvayo-Enríquez H, Mondaca-Fernández I, Gortáres-Moroyoqui P, Lopez-Cervantes J, Rodríguez-Ramírez R. Carotenoids extraction and quantification: a review. Anal Methods 2013; 5(12): 2916-2924. https://doi.org/10.1039/c3ay26295b
Othman R. Biochemistry and genetics of carotenoid composition in potato tubers. Lincoln University; 2009.
Wellburn AR. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J Plant Physiol 1994; 144(3): 307-313. https://doi.org/10.1016/S0176-1617(11)81192-2
Goiris K, Muylaert K, Fraeye I, Foubert I, De Brabanter J, De Cooman, L. Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. J Appl Phycol 2012; 24(6): 1477-1486. https://doi.org/10.1007/s10811-012-9804-6
Yoo KS, Bang H, Lee EJ, Crosby K, Patil BS. Variation of Carotenoid, Sugar, and Ascorbic Acid Concentrations in Watermelon Genotypes and Genetic Analysis. Hort Environ Biotechnol 2012; 53(6): 552-560. https://doi.org/10.1007/s13580-012-0014-6
Lester GE, Crosby KM.. Ascorbic acid, folic acid, and potassium content in postharvest green-flesh honeydew muskmelons: Influence of cultivar, fruit size, soil type, and year. J Amer Soc Hort Sci 2002; 127: 843-847. https://doi.org/10.21273/JASHS.127.5.843
Leskovar DI, Bang H, Crosby KM, Maness N, Franco JA, Perkins-Veazie P. Lycopene, carbohydrates, ascorbic acid and yield components of diploid and triploid watermelon cultivars are affected by deficit irrigation. J Hort Sci Biotechnol 2004; 79: 5-81. https://doi.org/10.1080/14620316.2004.11511739
Perkins-Veazie P, Collins JK, Davis AR, Roberts W. Carotenoid content of 50 watermelon cultivars. J Agr Food Chem 2006; 54: 2593-2597. https://doi.org/10.1021/jf052066p
Stepansky A, Kovalski I, Schaffer AA, Per-Treves R. Variation in sugar levels and invertase activity in mature fruit representing a broad spectrum of Cucumis melo genotypes. Genet Resour Crop Evolut 1999; 46: 53-62. https://doi.org/10.1023/A:1008636732481
Yativ M, Haray I, Wolf S. Sucrose accumulation in watermelon fruits: Genetic variation and biochemical analysis. J Plant Physiol 2010; 167: 589-596. https://doi.org/10.1016/j.jplph.2009.11.009
Dale MFB, Griffiths DW, Todd DT. Effects of Genotype, Environment, and Postharvest Storage on the Total Ascorbate Content of Potato (Solanum tuberosum) Tubers. J Agric Food Chem 2003; 51: 244-248. https://doi.org/10.1021/jf020547s
Hubert J, Galani Y, Mankad PM, Shah AK, Patel NJ, Acharya RR, Talati JG. Effect of Storage Temperature on Vitamin C, Total Phenolics, UPLC Phenolic Acid Profile and Antioxidant Capacity of Eleven Potato (Solanum tuberosum) Varieties. Hortic Plant J 2017; 3(2): 73-89. https://doi.org/10.1016/j.hpj.2017.07.004
Griffiths DW, Dale MF, Morris WL, Ramsay G. Effects of season and postharvest storage on the carotenoid content of Solanum phureja potato tubers. J Agric Food Chem 2007; 55(2): 379-85. https://doi.org/10.1021/jf0620822
Griffiths DW, Dale MFB. Effect of light exposure on glycoalkaloid content of Solanum phureja tubers. J Agric Food Chem 2001; 49: 5223-5227. https://doi.org/10.1021/jf010656r
Othman R, Mohd Hassan N, Mohd Hatta FA. Influence of Environmental Stress toward Carotenogenesis Regulatory Mechanism through In vitro Model System. In: Carotenoids. INTECH, Croatia, pp. 87-104.
Chloupek O, Hrstkova P. Adaptation of crops to environment. Theor Appl Genet 2005; 111: 1316-1321. https://doi.org/10.1007/s00122-005-0060-x
Lu W, Haynes K, Wiley E. Clevidence B. Carotenoid content and colour in diploid potatoes. J Amer Soc Hort Sci 2001; 126: 722-726. https://doi.org/10.21273/JASHS.126.6.722
Fraser PD, Bramley PM. The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 2004; 43: 228-265. https://doi.org/10.1016/j.plipres.2003.10.002
Havaux M, Niyogi KK. The violaxanthin cycle protects plants from photooxidative damage by more than one mechanism. Proc Natl Acad Sci USA 1999; 96: 8762-8767. https://doi.org/10.1073/pnas.96.15.8762
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