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Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Authors

  • Anton Tkachenko

    Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Trinklera st. 6, 61022 Kharkiv, Ukraine

  • Denys Pogozhykh

    Clinic for Hematology, Hemostaseology, Oncology and Stem Cell Transplantation, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

  • Anatolii Onishchenko

    Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Trinklera st. 6, 61022 Kharkiv, Ukraine

  • Valeriy Myasoedov

    Department of Medical Biology, Kharkiv National Medical University, Nauky ave. 4, 61022, Kharkiv, Ukraine

  • Leonid Podrigalo

    Department of Medical Science, Kharkiv State Academy of Physical Culture, Klochkovska str., 99, 61022, Ukraine

  • Vladimir Klochkov

    Yu.V. Malyukin Department of Nanostructured Materials, Institute for Scintillation Materials National Academy of Sciences of Ukraine, Nauky ave. 60, 61072 Kharkiv, Ukraine

  • Tetyana Chumachenko

    Department of Epidemiology, Kharkiv National Medical University, Trinklera st. 12, 61022 Kharkiv, Ukraine

  • Volodymyr Prokopyuk

    Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Trinklera st. 6, 61022 Kharkiv, Ukraine

  • Svetlana Yefimova

    Yu.V. Malyukin Department of Nanostructured Materials, Institute for Scintillation Materials National Academy of Sciences of Ukraine, Nauky ave. 60, 61072 Kharkiv, Ukraine

  • Galina Gubina-Vakulyck

    Department of Pathological Anatomy, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine

  • Nataliya Kavok

    Yu.V. Malyukin Department of Nanostructured Materials, Institute for Scintillation Materials National Academy of Sciences of Ukraine, Nauky ave. 60, 61072 Kharkiv, Ukraine

  • Dmytro Butov

    Department of Phthisiology and Pulmonology, Kharkiv National Medical University, Newton st. 145, 61000 Kharkiv, Ukraine

  • Andrii Andrieiev

    Department of Pathological Anatomy, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine

  • Hanna Polikarpova

    Department of Biochemistry, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine

  • Oksana Nakonechna

    Department of Biochemistry, Kharkiv National Medical University, Nauky ave. 4, 61022 Kharkiv, Ukraine

DOI:

https://doi.org/10.29169/1927-5951.2021.11.06

Keywords:

Carrageenan, food additive E407a, flow cytometry, apoptosis, necrosis, nanoparticles

Abstract

Gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation.

This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation.

Materials and Methods: Fragments of the small intestine of 8 rats treated orally with a carrageenan-containing food additive E407a at a dose of 140 mg / kg of weight during 2 weeks, 8 animals orally exposed to both E407a and VNPs at a dose of 20 μg / kg of weight during the same period of time, and 8 control rats were stained routinely and immunostained for CD3 and CD68 with the subsequent immunohistochemical scoring. Moreover, analysis of viability and cell death modes of granulocytes was performed by flow cytometry using Annexin V and 7-aminoactinomycin D (7-AAD).

Results: Oral exposure to the food additive E407a resulted in the development of enteritis associated with altered small intestinal morphology, infiltration of the lamina propria with macrophages and T-lymphocytes, and activation of peripheral blood granulocyte apoptosis. VNPs administered against the background of E407a-induced slight intestinal inflammation improved small intestinal morphology, decreased infiltration rate of the immune cells mentioned above without affecting the intensity of granulocyte apoptosis.

Conclusion: Oral administration of VNPs ameliorates E407a-induced enteritis.

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Published

2021-06-24

Issue

Vol. 11 (2021)

Section

Articles

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How to Cite

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation. (2021). Journal of Pharmacy and Nutrition Sciences , 11, 40-48. https://doi.org/10.29169/1927-5951.2021.11.06

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