A simple precise, accurate RP-HPLC method has been developed and validated for analysis of Zolmitriptan (ZLM). The separation and quantization were achieved on a 250 mm reversed phase column with a hydrophilic linkage between silica particles and hydrophobic alkyl chains. The mobile phase was constituted (flow rate 0.8 ml min-1) of eluant A (CH3OH) and eluant B (aqueous tetra butyl ammonium hydrogen sulphate) (pH 3.4; 10 mM) using isocratic elution with UV detection at 224 nm. The method showed good linearity for ZMT in the 1–100 μg mL-1 range with regression equation 15576x ± 99401 and correlation coefficient 0.999 respectively. The limit of quantitation (LOQ) and limit of detection (LOD) were found to be 0.8134 and 0.2687 μg mL-1 respectively. Finally the applicability of the method was validated according to ICH guidelines and can be applicable for the analysis of commercial dosage forms.
Oldman AD, Smith LA, McQuay HJ and Moore RA. 2002. Pharmacological treatments for acute migraine: quantitative systematic review. Pain. 97: 247-257.
Xu, G.Y.; Zhou, Y.; Xu, M.C. A convenient synthesis of antibacterial Zolmitriptan from (S)-glyceraldehyde acetonide. Chin. Chem. Lett. 2006, 17, 302–304.
Yates R, Nairn K, Dixon R, Kemp JV, Dane AL. Pharmacokinetics, Dose Proportionality, and Tolerability of Single and Repeat Doses of a Nasal Spray Formulation of Zolmitriptan in Healthy Volunteers. J. Clin. Pharmacol. 2002, 42, 1244-1250.
Vishwanathan K, Bartlett MG and Stewart JT. Determination of antimigraine compounds rizatriptan, zolmitriptan, naratriptan and sumatriptan in human serum by liquid chromatography/electrospray tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2000, 14, 168-172.
Chen X, Liu D, Luan Y, Jin F and Zhong D. Determination of zolmitriptan in human plasma by liquid chromatography– tandem mass spectrometry method: Application to a pharmacokinetic study. J. Chromatogr. B. 2006, 832, 30-35.
Zhang Z, Xu F, Tian Y, Li W and Mao G. Quantification of zolmitriptan in plasma by high-performance liquid chromatography–electrospray ionization mass spectrometry. J. Chromatogr. B. 2004, 813, 227-233.
Yao JC, Yan-hui QU, Zhao XY, Hu L, Zhu R, Li H, Ding J. Determination of Zolmitriptan in Human Plasma by High- Performance Liquid Chromatography-Electrospray Mass Spectrometry and Study on Its Pharmacokinetics. J. Chinese Pharm. Sci. 2005, 14, 25-28.
Srinivasu MK, Rao BM, Sridhar G, Chandrasekhar KB and Kumar PR. A validated chiral LC method for the enantiomeric separation of Zolmitriptan key intermediate, ZTR-5. J. Pharm. Biomed. Anal. 2005, 39, 796-800.
Srinivasu MK, Rao BM, Sridhar G, Kumar PR, Chandrasekhar KB and Islam A. A validated chiral LC method for the determination of Zolmitriptan and its potential impurities. J. Pharm. Biomed. Anal. 2005, 37, 453-460.
Yu L, Yao T, Ni S, Zeng S. Determination of zolmitriptan enantiomers in rat liver microsomes by chiral high performance liquid chromatography with fluorescence detection. Biomed. Chromatogr. 2005, 19, 191–195.
Chen J, Jiang X, Jiang W, Mei N, Gao X and Zhang Q. Highperformance liquid chromatographic analysis of zolmitriptan in human plasma using fluorescence detection. J. Pharm. Biomed. Anal. 2004, 3, 639-645.
Hu YZ, Yao TW and Wang XJ. HPLC determination of zolmitriptan and its related substances. Zhejiang Da Xue Bao Yi Xue Ban. 2004, 33, 37-40.
Vijayakumar EKS, Samel MA, Bhalekar SB, Pakhale SM. A new stability indicating HPLC method for related substances in zolmitriptan. Indian J Pharm Sci. 2010, 72, 119-122.
Clement EM and Franklin M. Simultaneous measurement of zolmitriptan and its major metabolites Ndesmethylzolmitriptan and zolmitriptan N-oxide in human plasma by high-performance liquid chromatography with coulometric detection. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2002, 766, 339-343.
Raza A, Ansari TM and Niazi SB. Novel Spectrophotometric Method for the Determination of Zolmitriptan in Pharmaceutical Formulations. J. Chinese Chemical Society. 2007, 54, 1413-1417.
Induri MR, Raju MB, Prasad YR, Reddy KP, Boddu R and Raidu ChS. A validated RP-HPLC method for the quantification of Zolmitriptan in tablet dosage form. Der Pharma Chemica. 2010, 2, 351-357.
Rao BM, Srinivasu MK, Sridhar G, Kumar PR, Chandrasekhar KB and Islam A. A stability indicating LC method for zolmitriptan. J. Pharm. Biomed. Anal. 2005, 39, 503-509.
Aydogmus Z, Inanli I. ExtZLMtive spectrophotometric methods for determination of zolmitriptan in tablets. J AOAC Int. 2007, 90, 1237-1241.
Pang N, Zhang Z, Bai Y and Liu H. (2009). A study of the inteZLMtion between enantiomers of zolmitriptan and hydroxypropyl-beta-cyclodextrin by capillary electrophoresis. Anal Bioanal Chem. 393: 313-320.
International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceutical for Human Use: Validation of Analytical procedures, Text and methodology - Q2 (R1), 2005.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.