A Generalized Review on Extraction of Biomolecules

Authors

  • S. Shireen Food and Nutrition, Foozie Fit. Kandivali (W) Mumbai-400057, Maharastra, India
  • A.S. Zarena Department of Biotechnology, Teresian college. Mysore-570011, Karnataka, India

DOI:

https://doi.org/10.29169/1927-5951.2022.12.15

Keywords:

Extraction, Tools, Purification, Chromatography

Abstract

Nature has given us a wide range of biological compounds that can be utilized to help combat health problems, but sometimes with over-processing, these advantages are diminished or lost. Food and pharmaceutical companies have developed a range of new approaches to harness and retain the naturally occurring diversity and quality of bioactive compounds efficiently and effectively. Pharmaceutically important plant products have been known for millennia; they have been used in crude and unrefined forms. One of the best ways to pick the best plant bioactive is through genetic engineering, omics, and plant tissue culture. Many laboratories routinely screen plant species for bioactive compounds to discover new ones. All extraction methods depend on the researcher's preference and what exactly the research entails. Successful extraction begins with the careful selection and preparation of plant samples and thorough knowledge and review of the appropriate literature. Here we have attempted to describe the different stages and methods of extraction from the medicinal plants. From the review, it can be concluded that no universal extraction method is ideal and that each extraction procedure is unique.

References

Muzzaffar S, Azam I. Health and Medicinal Properties of Saffron. In Handbook of Oleoresins. CRC Press pp. 63-93. https://doi.org/10.1201/9781003186205-4 DOI: https://doi.org/10.1201/9781003186205-4

Lou Q, Liu H, Luo W, Chen K, Liu Y. Creating a novel petal regeneration system for function identification of colour gene of grape hyacinth. Plant Methods 2021; 17(1): 1-0. https://doi.org/10.1186/s13007-021-00794-7 DOI: https://doi.org/10.1186/s13007-021-00794-7

Zarena AS. Exploring the Potential Bioactive Properties of Marine Natural Products. Current Bioactive Compounds 2019; 15(5): 524-39. https://doi.org/10.2174/1573407214666180727092555 DOI: https://doi.org/10.2174/1573407214666180727092555

Daud NM, Putra NR, Jamaludin R, Norodin NS, Sarkawi NS, Hamzah MH, Nasir HM, Zaidel DN, Yunus MA, Salleh LM. Valorisation of plant seed as natural bioactive compounds by various extraction methods: A review. Trends in Food Science & Technology 2022; 119: 201-14. https://doi.org/10.1016/j.tifs.2021.12.010 DOI: https://doi.org/10.1016/j.tifs.2021.12.010

Zarena AS, Sankar KU. A study of antioxidant properties from Garcinia mangostana L. pericarp extract. Acta Scientiarum Polonorum Technologia Alimentaria 2009; 8(1): 23-34.

Tzanova M, Atanasov V, Yaneva Z, Ivanova D, Dinev T. Selectivity of current extraction techniques for flavonoids from plant materials. Processes 2020; 8(10): 1222. https://doi.org/10.3390/pr8101222 DOI: https://doi.org/10.3390/pr8101222

Hayat J, Akodad M, Moumen A, Baghour M, Skalli A, Ezrari S, Belmalha S. Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubiumvulgare L. from 2 different localities of Northeast of Morocco. Heliyon 2020; 6(11): e05609. https://doi.org/10.1016/j.heliyon.2020.e05609 DOI: https://doi.org/10.1016/j.heliyon.2020.e05609

Nwakiban AP, Sangiovanni E, Piazza S, Fumagalli M, Khalilpour S, Martinelli G, Magnavacca A, Beretta G, Agbor GA, Kuiaté JR, Dell’Agli M. Antioxidant and anti-inflammatory activities of Cameroon nutritional spice extracts in human gastric epithelial cells. Planta Medica 2019; 85(18): SL-AR. https://doi.org/10.1055/s-0039-3399631 DOI: https://doi.org/10.1055/s-0039-3399631

Zarena AS, Bhattacharya S, Kadimi US. Mangosteen oil-in-water emulsions: rheology, creaming, and microstructural characteristics during storage. Food and Bioprocess Technology 2012; 5(8): 3007-13. https://doi.org/10.1007/s11947-011-0695-3 DOI: https://doi.org/10.1007/s11947-011-0695-3

Zarena AS, Manohar B, UdayaSankar K. Optimization of supercritical carbon dioxide extraction of xanthones from mangosteen pericarp by response surface methodology. Food and Bioprocess Technology 2012; 5(4): 1181-8. https://doi.org/10.1007/s11947-010-0404-7 DOI: https://doi.org/10.1007/s11947-010-0404-7

Handa SS. An overview of extraction techniques for medicinal and aromatic plants. Extraction technologies for medicinal and aromatic plants. United Nations Industrial Development Organization 2008; 1: 21-40.

Jha AK, Sit N. Extraction of bioactive compounds from plant materials using combination of various novel methods: A review. Trends in Food Science & Technology 2021. https://doi.org/10.1016/j.tifs.2021.11.019 DOI: https://doi.org/10.1016/j.tifs.2021.11.019

Zarena AS, Gopal S, Vineeth R. Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein. Journal of Analytical Methods in Chemistry 2014; 2014. https://doi.org/10.1155/2014/989543 DOI: https://doi.org/10.1155/2014/989543

Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. Journal of Pharmacy & Bioallied Sciences 2020; 12(1): 1. https://doi.org/10.4103/jpbs.JPBS_175_19 DOI: https://doi.org/10.4103/jpbs.JPBS_175_19

Sharma M, Kaushik P. Vegetable phytochemicals: An update on extraction and analysis techniques. Biocatalysis and Agricultural Biotechnology 2021; 36: 102149. https://doi.org/10.1016/j.bcab.2021.102149 DOI: https://doi.org/10.1016/j.bcab.2021.102149

Zarena AS, Sankar KU. Screening of xanthone from mangosteen (Garcinia mangostana L.) peels and their effect on cytochrome c reductase and phosphomolybdenum activity. Journal of Natural Products (India) 2009; 2: 23-30.

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Medicine 2018; 13(1): 1-26. https://doi.org/10.1186/s13020-018-0177-x DOI: https://doi.org/10.1186/s13020-018-0177-x

Akhtar I, Javad S, Yousaf Z, Iqbal S, Jabeen K. Microwave assisted extraction of phytochemicals an efficient and modern approach for botanicals and pharmaceuticals. Pakistan Journal of Pharmaceutical Sciences 2019; 32(1).

Llompart, Maria, Carmen Garcia-Jares, MaríaCeleiro, and Thierry Dagnac. Extraction| Microwave-assisted extraction. 2019; 67-77. https://doi.org/10.1016/B978-0-12-409547-2.14442-7 DOI: https://doi.org/10.1016/B978-0-12-409547-2.14442-7

Prandi B, Di Massimo M, Tedeschi T, Rodríguez-Turienzo L, Rodríguez Ó. Ultrasound and Microwave-Assisted Extraction of Proteins from Coffee Green Beans: Effects of Process Variables on the Protein Integrity. Food and Bioprocess Technology 2022; 15(12): 2712-22. https://doi.org/10.1007/s11947-022-02907-z DOI: https://doi.org/10.1007/s11947-022-02907-z

Du C, Wang J, Wang Y, Deng J, Luo G. Microdroplet-based continuous countercurrent extraction with high phase ratio. Separation and Purification Technology 2022; 121269. https://doi.org/10.1016/j.seppur.2022.121269 DOI: https://doi.org/10.1016/j.seppur.2022.121269

Wang F, Cheng L, Cao Y, Wei Q, Tong C, Shi S. Online extraction and enrichment coupling with high‐speed counter‐current chromatography for effective and target isolation of antitumor anthraquinones from seeds of Cassia obtusifolia. Journal of Separation Science 2022; 45(4): 938-44. https://doi.org/10.1002/jssc.202100775 DOI: https://doi.org/10.1002/jssc.202100775

Zarena AS, Sankar KU. Xanthones enriched extracts from mangosteen pericarp obtained by supercritical carbon dioxide process. Separation and Purification Technology 2011; 80(1): 172-8. https://doi.org/10.1016/j.seppur.2011.04.027 DOI: https://doi.org/10.1016/j.seppur.2011.04.027

Zarena AS, Sankar KU. Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: characterization by HPLC/LC–ESI-MS. The Journal of Supercritical Fluids 2009; 49(3): 330-7. https://doi.org/10.1016/j.supflu.2009.03.004 DOI: https://doi.org/10.1016/j.supflu.2009.03.004

Bartella L, Mazzotti F, Talarico IR, Santoro I, Di Donna L. Hydroxytyrosol-Fortified Foods Obtained by Supercritical Fluid Extraction of Olive Oil. Antioxidants 2021; 10(10): 1619. https://doi.org/10.3390/antiox10101619 DOI: https://doi.org/10.3390/antiox10101619

Lefebvre T, Destandau E, Lesellier E. Sequential extraction of carnosic acid, rosmarinic acid and pigments (carotenoids and chlorophylls) from Rosemary by online supercritical fluid extraction-supercritical fluid chromatography. Journal of Chromatography A 2021; 1639: 461709. https://doi.org/10.1016/j.chroma.2020.461709 DOI: https://doi.org/10.1016/j.chroma.2020.461709

Jumaah F, Sandahl M, Turner C. Supercritical fluid extraction and chromatography of lipids in bilberry. Journal of the American Oil Chemists' Society 2015; 92(8): 1103-11. https://doi.org/10.1007/s11746-015-2680-x DOI: https://doi.org/10.1007/s11746-015-2680-x

Das G, Patra JK, Kang SS, Shin HS. Pharmaceutical importance of some promising plant species with special reference to the isolation and extraction of bioactive compounds: A review. Current Pharmaceutical Biotechnology 2022; 23(1): 15-29. https://doi.org/10.2174/1389201022666210122125854 DOI: https://doi.org/10.2174/1389201022666210122125854

Su Y, Xia S, Wang R, Xiao L. Phytohormonal quantification based on biological principles. Hormone Metabolism and Signaling in Plants 2017; 13: 431-70. https://doi.org/10.1016/B978-0-12-811562-6.00013-X DOI: https://doi.org/10.1016/B978-0-12-811562-6.00013-X

De La Cruz-Jiménez L, Hernández-Torres MA, Monroy-García IN, Rivas-Morales C, Verde-Star MJ, Gonzalez-Villasana V, Viveros-Valdez E. Biological activities of seven medicinal plants used in Chiapas, Mexico. Plants 2022; 11(14): 1790. https://doi.org/10.3390/plants11141790 DOI: https://doi.org/10.3390/plants11141790

Rito-Palomares M, Benavides J, editors. Aqueous two-phase systems for bioprocess development for the recovery of biological products. Cham: Springer International Publishing 2017. https://doi.org/10.1007/978-3-319-59309-8 DOI: https://doi.org/10.1007/978-3-319-59309-8

Iqbal M, Tao Y, Xie S, Zhu Y, Chen D, Wang X, Huang L, Peng D, Sattar A, Shabbir MA, Hussain HI. Aqueous two-phase system (ATPS): an overview and advances in its applications. Biological Procedures Online 2016; 18(1): 1-8. https://doi.org/10.1186/s12575-016-0048-8 DOI: https://doi.org/10.1186/s12575-016-0048-8

Chen C, Tian H, Xing S, Li C, Zeng X, He L. Influence of different parameters on reverse micelle extraction combined with acetone precipitation to purify sn-1, 3 extracellular lipase from Aspergillus niger GZUF36. Journal of Food Science and Technology 2019; 56(6): 2899-908. https://doi.org/10.1007/s13197-019-03743-4 DOI: https://doi.org/10.1007/s13197-019-03743-4

Walter H, editor. Partitioning in aqueous two–phase system: theory, methods, uses, and applications to biotechnology Elsevier 2012.

Tan SC, Leow JW, Lee HK. Emulsification-assisted micro-solid-phase extraction using a metal-organic framework as sorbent for the liquid chromatography-tandem mass spectrometric analysis of polar herbicides from aqueous samples. Talanta 2020; 216: 120962. https://doi.org/10.1016/j.talanta.2020.120962 DOI: https://doi.org/10.1016/j.talanta.2020.120962

Vas G, Vekey K. Solid‐phase microextraction: a powerful sample preparation tool prior to mass spectrometric analysis. Journal of Mass Spectrometry 2004; 39(3): 233-54. https://doi.org/10.1002/jms.606 DOI: https://doi.org/10.1002/jms.606

Naliyadhara N, Kumar A, Girisa S, Daimary UD, Hegde M, Kunnumakkara AB. Pulsed electric field (PEF): Avant-garde extraction escalation technology in food industry. Trends in Food Science & Technology 2022. https://doi.org/10.1016/j.tifs.2022.02.019 DOI: https://doi.org/10.1016/j.tifs.2022.02.019

Cheng Z, Jiang H. Supported liquid extraction (SLE) in LC‐MS bioanalysis. Sample Preparation in LC‐MS Bioanalysis 2019; 76-84. https://doi.org/10.1002/9781119274315.ch6 DOI: https://doi.org/10.1002/9781119274315.ch6

Łubek-Nguyen A, Ziemichód W, Olech M. Application of enzyme-assisted extraction for the recovery of natural bioactive compounds for nutraceutical and pharmaceutical applications. Applied Sciences 2022; 12(7): 3232. https://doi.org/10.3390/app12073232 DOI: https://doi.org/10.3390/app12073232

Pingret D, Fabiano-Tixier AS, Chemat F. Ultrasound-assisted extraction. Natural Product Extraction: Principles and Applications 2013; 21: 89. https://doi.org/10.1039/9781849737579-00089 DOI: https://doi.org/10.1039/9781849737579-00089

Robards K, Ryan D. Principles and practice of modern chromatographic methods. Academic Press 2021. https://doi.org/10.1016/B978-0-12-822096-2.00005-0 DOI: https://doi.org/10.1016/B978-0-12-822096-2.00005-0

Azmi FI, Goh PS, Ismail AF, Hilal N, Wong TW, Misson M. Biomolecule-Enabled Liquid Separation Membranes: Potential and Recent Progress. Membranes 2022; 12(2): 148. https://doi.org/10.3390/membranes12020148 DOI: https://doi.org/10.3390/membranes12020148

Stanbury PF, Whitaker A, Hall SJ. Principles of fermentation technology. Elsevier 2013.

Parhizi Z, Karami H, Golpour I, Kaveh M, Szymanek M, Blanco-Marigorta AM, Marcos JD, Khalife E, Skowron S, Adnan Othman N, Darvishi Y. Modeling and optimization of energy and exergy parameters of a hybrid-solar dryer for basil leaf drying using RSM. Sustainability 2022; 14(14): 8839. https://doi.org/10.3390/su14148839 DOI: https://doi.org/10.3390/su14148839

Verri Jr WA, Vicentini FT, Baracat MM, Georgetti SR, Cardoso RD, Cunha TM, Ferreira SH, Cunha FQ, Fonseca MJ, Casagrande R. Flavonoids as anti-inflammatory and analgesic drugs: mechanisms of action and perspectives in the development of pharmaceutical forms. Studies in Natural Products Chemistry 2012; 36: 297-330. https://doi.org/10.1016/B978-0-444-53836-9.00026-8 DOI: https://doi.org/10.1016/B978-0-444-53836-9.00026-8

Valkó K. Separation methods in drug synthesis and purification. Elsevier 2000.

https://www.separations.eu.tosohbioscience.com/ accessed on 01/11/2022

Molineau J, Hideux M, West C. Chromatographic analysis of biomolecules with pressurized carbon dioxide mobile phases–A review. Journal of Pharmaceutical and Biomedical Analysis 2021; 193: 113736. https://doi.org/10.1016/j.jpba.2020.113736 DOI: https://doi.org/10.1016/j.jpba.2020.113736

McPherson RA, Pincus MR. Henry's clinical diagnosis and management by laboratory methods E-book. Elsevier Health Sciences 2021.

Proudfoot A, Frank AO, Frommlet A, Lingel A. Selective methyl labeling of proteins: enabling structural and mechanistic studies as well as drug discovery applications by solution-state NMR. In Methods in Enzymology 2019; (Vol. 614, pp. 1-36). Academic Press. https://doi.org/10.1016/bs.mie.2018.08.035 DOI: https://doi.org/10.1016/bs.mie.2018.08.035

Bruno A, Velders AH, Biasone A, Li Vigni M, Mondelli D, Miano T. Chemical Composition, Biomolecular Analysis, and Nuclear Magnetic Resonance Spectroscopic Fingerprinting of Posidonia oceanica and Ascophyllumn odosum Extracts. Metabolites 2023; 13(2): 170. https://doi.org/10.3390/metabo13020170 DOI: https://doi.org/10.3390/metabo13020170

van der Wel PC. New applications of solid-state NMR in structural biology. Emerging topics in life sciences 2018; 2(1): 57-67. https://doi.org/10.1042/ETLS20170088 DOI: https://doi.org/10.1042/ETLS20170088

Smyth MS, Martin JH. x Ray crystallography. Molecular Pathology 2000; 53(1): 8. https://doi.org/10.1136/mp.53.1.8 DOI: https://doi.org/10.1136/mp.53.1.8

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Published

2022-12-27

How to Cite

Shireen, S., & Zarena, A. (2022). A Generalized Review on Extraction of Biomolecules . Journal of Pharmacy and Nutrition Sciences, 12, 175–187. https://doi.org/10.29169/1927-5951.2022.12.15

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Section

Special Issue: Secondary Metabolites: Detection and Bioactivity