Antioxidant Effect of a Combination of S-Acetyl-L-Glutathione, Vitamin E, Silybum Marianum on Hepatic Cells under Oxidative Stress: An In Vitro Study


  • N. Lonigro Department of Drug Science and Technology, University of Turin, Turin, Italy
  • Z. R. Mohammed Candioli Pharma S.r.l., Beinasco (TO), Italy
  • F. Perondi Department of Veterinary Science, University of Pisa, San Piero a Grado (Pisa), Italy
  • E. Martello Division of Epidemiology and Public Health, School of Medicine, University of Nottingham, UK
  • A. Costale Department of Drug Science and Technology, University of Turin, Turin, Italy
  • E. Rosso Department of Drug Science and Technology, University of Turin, Turin, Italy
  • N. Bruni Candioli Pharma S.r.l., Beinasco (TO), Italy



pet, liver, oxidative stress, ROS, Supplement, natural ingredient


Oxidative stress plays a key role in the pathogenesis of liver diseases and can be involved in the inflammatory process of liver cells. The aim of this vitro study is to assess the antioxidant efficacy of three distinct components (fermented S-Acetyl -L-glutathione, Silybum marianum (L.) Gaern. and vitamin E all-rac-alpha-tocopheryl acetate) both individually and in combination (Glutasyl product). In addition, we also evaluated the combined antioxidant effect of the three ingredients on human hepatic cells subjected to oxidative stress induced by H2O2 treatment.

The results showed the dose-dependent antioxidant potential of the three components suggesting promising applications in medical contexts. The synergistic antioxidant effects observed for the Glutasyl was more than the individual components. Additionally, Glutasyl showed not-toxic activity on human hepatocytes and it could be considered useful in mitigating cytotoxicity induced by oxidative stress.


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How to Cite

Lonigro, N., Mohammed , Z. R., Perondi, F., Martello, E., Costale, A., Rosso, E., & Bruni, N. (2024). Antioxidant Effect of a Combination of S-Acetyl-L-Glutathione, Vitamin E, Silybum Marianum on Hepatic Cells under Oxidative Stress: An In Vitro Study . Journal of Pharmacy and Nutrition Sciences, 14, 1–7.