Abstract
Introduction: Meat is an important source of nutrition for many people worldwide. Away from homes, it is widely consumed in restaurants (e.g. small roadside) and non-restaurants (including drinking parlors and open-air)in various forms assnack. Objective: This paper reports on the acute toxicity of mycotoxigenic fungi isolates from various ready-to-eat meat from Buea, Cameroon.
Method: Ten varieties of ready-to-eat meat samples (N=130: raw beef (n=18), beef soya (n=29), fried chicken (n=11), chicken soya (n=10), snail soya (n=13), fried pork (n=06), fried cow liver (n=13), roasted goat (n=06), boiled cow skin (n=07) and smoked beef (n=17).were collected from Buea municipality. The samples were cultures, and fungal isolates isolated microscopically. Thereafter, an acute toxicity test using 24 young BALB/c mice (weight range: 22-25g) was performed.
Results: A total of 40 mycotoxigenic fungi belonging to five genera (Aspergillus, Penicillium, Fusarium, Rhizomucor, and Rhizopus) were identified. The most dominant species was Aspergillus (unidentified Aspergillus species, 23.1%; and Aspergillus ochraceus,10.3%).The order of Aspergillus fungi proliferation were roasted goat liver(20%), fried chicken (20%), snails soya (20%)ean body weight of test mice significantly decreased (p<0.05) after the first 7 days of feeding with a mycotoxigenic fungi-supplemented diet relative to control mice that ate healthy diets. Aspartate Transaminase (AST, range: 169.9-563.1U/L) and Alanine Transaminase (ALT, range:521.1-707.5U/L) levels were significantly (p<0.05) higher than the reference values for healthy mice (range: 60-100U/L for AST and 25-6U/L for ALT).
Conclusion: Given the toxicity potentials of mycotoxigenic fungi, e.g. production of toxic secondary metabolites, not reported in this paper, and demonstrated by the liver toxicity/function test, ready-to-eat meat in Buea, Cameroon may not be adequately safe for consumption.
References
Ritchie H, Poser M. Meat and Dairy Production 2019. Available from https://ourworldindata.org/meat-production [accessed 10 December 2022].
World Health Organization. World health report. Geneva: The Organization; 2002.
Brabin BJ, Coulter JBS. Nutrition-associated disease. In: Cook GC, Zumla AI, editors. Manson's tropical diseases. London: Saunders; 2003; 561-80.
World Health Organization, United Nations Children's Fund. Joint statement on the management of acute diarrhoea. Geneva: The Organization; 2004.
FAO (Food and Agriculture Organization). Worldwide regulations for mycotoxins in food and feed in 2003. Rome: FAO. Food and Nutrition Papers 2004; No. 81.
Millward DJ, Jackson AA. Protein/energy ratios of current diets in developed and developing countries compared with a safe protein/energy ratio: implications for recommended protein and amino acid intakes. Public Health Nutr 2004; 7:387-405. https://doi.org/10.1079/PHN2003545
Abia WA, Wanji S, Tcheuntue F. Energy and nutrient contents of waterfufu and eru. African Journal of Food Science 2007; 1(2): 016-019.
Ong S, Loo L, Pang M, Tan R,Teng Y, Lou X, Chin SK, Naik MY, Yu H. Decompartmentalisation as a simple colour manipulation of plant-based marbling meat alternatives. Biomaterials 2021; 277: 121107. https://doi.org/10.1016/j.biomaterials.2021.121107
Wang SK, Fu LM, Chen GW, Xiao HM, Pan D, Shi RF, Yang LG, Sun GJ. Multisite survey of bacterial contamination in ready-to-eat meat products throughout the cooking and selling processes in urban supermarket, Nanjing, China. Food Sci Nutr. 2020; 8(5): 2427-2435. https://doi.org/10.1002/fsn3.1532
Park JW, Choi S, Hwang H, Kim Y. Fungal mycoflora and mycotoxins in Korean polished rice destined for humans. International Journal of Food Microbiology 2005; 103: 305-314. https://doi.org/10.1016/j.ijfoodmicro.2005.02.001
Mastanjević K, Kovačević D, Nešić K, Krstanović V, Habschied K. Traditional Meat Products-A Mycotoxicological Review. Life (Basel) 2023; 13(11): 2211. https://doi.org/10.3390/life13112211
Alshannaq A, Yu JH. Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food. Int J Environ Res Public Health 2017; 14(6): 632. https://doi.org/10.3390/ijerph14060632
Hawksworth DL. The fungal dimension of biodiversity: magnitude, significance and conservation. Mycological Research 1991; 95: 641-655. https://doi.org/10.1016/S0953-7562(09)80810-1
Marais GJ. (2000). Natural occurrence of mycotoxins in foods and feeds in South Africa. Submission to AFMA Technical Committee, October 2000.
Hawksworth DL. Fungal diversity and its implications for genetic resources collections. Studies in Mycology 2004; 50: 9-18.
Blaha J, Tamchinova J, Reisnerova H. The occurrence of moulds and aflatoxin B1in Vietnamese feeds. Journal of Tropical Science 1990; 30: 21-31.
Njobeh PB. (2003). Influence of composition and storage conditions on diet quality and productivity of broiler chickens. Thesis written in fulfilment of the requirements for the degree of Master of Science in the Discipline of Animal and Poultry Science, School of Agricultural Science and Agribusiness, University of Natal, Pietermaritzburg, South Africa.
Filtenborg O, Frisvad TC, Samson RA. Specific association of fungi in foods and influence of physical environmental factors. In: Introduction to Food- and Airborne Fungi 6th Edition. Samson, R. A., Hoekstra, E. S., Frisvad, J. C. and Filtenborg, O. (Eds.). Centralbureau voor Schimmelculture, Utrecht 2000; 306-320.
Ayuk-Takem JA, Eckebil JP, Chheda HR. Problems and potentials of maize (Zea mays L.) research and production in Cameroon. Review of Scientific Technology 1982; 2: 5-16.
Sayed MA, Mohamoud EL A., Abou- EIAlIa AA. Mycoflora and natural occurrence of mycotoxins in meat and livers of imported bulls, poultry and some meat products. Assuit Vet Med J 2000; 43: 188-200. https://doi.org/10.21608/avmj.2000.182028
Abia WA, Warth B, Sulyok M, Krska R, Tchana A, Njobeh PB, Turner PC, Kouanfack C, Eyongetah M, Dutton M, Moundipa PF. Bio-monitoring of mycotoxin exposure in Cameroon using a urinary multi-biomarker approach. Food and Chemical Toxicology 2013b; 62: 927-934. https://doi.org/10.1016/j.fct.2013.10.003
Tchana NA, Moundipa PF, Tchouanguep FM. Aflatoxin Contamination in Food and Body Fluids in Relation to Malnutrition and Cancer Status in Cameroon. International Journal of Environmental Research and Public Health 2010; 7: 178-188. https://doi.org/10.3390/ijerph7010178
Abia WA, Warth B, Sulyok M, Krska R, Tchana NA, Njobeh PB, Dutton MF, Moundipa PF. Determination of multi-mycotoxin occurrence in cereals, nuts and their products in Cameroon by liquid chromatography tandem mass spectrometry (LC-MS/MS). Food Control 2013a; 31: 438-453. https://doi.org/10.1016/j.foodcont.2012.10.006
Abia WA, Warth B, Ezekiel CN, Sarkanj B, Turner PC, Marko D, Krska R, Sulyok M. Uncommon toxic microbial metabolite patterns in traditionally home-processed maize dish (fufu) consumed in rural Cameroon. Food and Chemical Toxicology (Part A) 2017; 107:10-19. https://doi.org/10.1016/j.fct.2017.06.011
Ekwa Y. Monono, Wilfred A. Abia, Ngomba DM Mokake, Yannick A. Afanga, Eyole N. Monono, Ojong A. Ntane, Kame HL Monono, Jemimah E. Ngale, Marie EI Ebobisse, Sally A. Sumbele, Dopgima L. Levai. Aflatoxins in Cameroon: Occurrence, Potential Health Risks and the Way Forward in Implementing the Partnership for Aflatoxin Control in Africa (PACA) Country-Led Model. Journal of Food and Nutrition Sciences 2024; 12: 222-233. https://doi.org/10.11648/j.jfns.20241205.13
Abia WA. Aflatoxins: Probing the multifaceted impacts and innovative solutions for agri-food security and safety, economic stability, trade and public health in Cameroon. Journal of Nutrition and Diet Management 2024; 2:1-9
Essono G, Ayodele M, Akoa A, Foko J, Olembo S, Gockowski J. Aspergillus species on cassava chips in storage in rural areas of southern Cameroon: their relationship with storage duration, moisture content and processing methods. African Journal of Microbiology Research 2007; 1: 001-008.
Njobeh PB, Dutton MF, Koch SH, Chuturgoon AA, Stoev SD, Seifert K. Contamination wwithith storage fungi of human food from Cameroon. International Journal of Food Microbiology 2009; 135: 193-198. https://doi.org/10.1016/j.ijfoodmicro.2009.08.001
Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CH, Perrone G, Seifert KA, Varga J, Yaguchi T, Samson RA. Identification and nomenclature of the genus Penicillium. Stud Mycol 2014; 78: 343-71. https://doi.org/10.1016/j.simyco.2014.09.001
Molua EL, Lambi CM. The economic impact of climate change on agriculture in Cameroon. CEEPA Discussion Paper No. 17, Centre for Environmental Economics and Ploicy in Africa, University of Pretoria.
Pitt JI, Hocking AD. Fungi and Food Spoilage, 2nd edition. Blackie Academic and Professional, an imprint of Chapman and Hall, London, UK 1997; 173- 366. https://doi.org/10.1007/978-1-4615-6391-4_6
Okungbowa FI. Rhizopus, Aspergillus and Candida implicated in the protein loss of stored cotton(Gossypium hirsutum L.) seeds at ambient temperature. African Journal of Agriculture Research 2005; 1: 1-6.
Jimoh KO, Kolapo AL. Mycoflora and aflatoxin production in market samples of some selected Nigerian foodstuffs. Research Journal of Microbiology 2008; 3(3): 169-174. https://doi.org/10.3923/jm.2008.169.174
Darwish S, El Bayomi M, Abd El-Moaty M, Gad M. Mould contamination and aflatoxin residues in frozen chicken meat-cuts and giblets. Japanese Journal of Veterinary Research 2016; 64(Supplement 2): S167-171.
Ismail MA, Zaky ZM .Evaluation of the mycological status of luncheon meat with special reference to aflatoxigenicmoulds and aflatoxin residues. Mycopathologia 1999; 146: 147-154. https://doi.org/10.1023/A:1007086930216
Mizakova A, Pipova M, Turek P. The occurrence of moulds in fermented raw meat products. Czech J. Food Sci 2002; 3: 89-94. https://doi.org/10.17221/3516-CJFS
Pitt JI, Hocking AD. (2009). Fungi and Food spoilage. 3rd Ed Springer Science +Business Media, LLC, 233 Spring Street, New York, NY 10013, USA. https://doi.org/10.1007/978-0-387-92207-2_2
Iacumin L, Chiesa L, Boscolo D, Manzano M, Cantoni C, Orlic S, Comi G Moulds and ochratoxin A on surfaces of artisanal and industrial dry sausages. Food Microbiol 2009; 26: 65-70. https://doi.org/10.1016/j.fm.2008.07.006
Sopandi T, Wardah A. Sugar consumption in mono and co-culture Saccharomyces cerevisiae and others selected microorganism for bioethanol production from stream rice husk medium. Asian. J. Microbiol. Biotechnol. Environ. Sci 2015; 17(3): 89-98.
Adeniyi AF, Adeleye JO, Adeniyi CY. Diabetes, Sexual Dysfunction and Therapeutic Exercise: A 20 Year Review. Current Diabetes Reviews 2010; 6: 201-206. https://doi.org/10.2174/157339910791658907

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