Phytochemical and Biological Investigations of Different Partitioned Extracts of Ficus heteropleura Blume (Family: Moraceae) Leaves
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Keywords

Antidiabetic
Thrombolytic
Antimicrobial
Phytoconstituent
Anti-inflammatory
Ficus heteropleura
Membrane stabilizing

How to Cite

Nath, P. P., Hossen, M. S., Papia, S. A., Trisha, S. Y., Sultana, I., & Ibrahim, M. (2024). Phytochemical and Biological Investigations of Different Partitioned Extracts of Ficus heteropleura Blume (Family: Moraceae) Leaves. Journal of Pharmacy and Nutrition Sciences, 14, 90–99. https://doi.org/10.29169/1927-5951.2024.14.11

Abstract

Ficus heteropleura Blume is a plant of the Moraceae family with high medicinal value. Therefore, the plant leaves' fractional extracts have been subjected to preliminary screenings for phytoconstituents and pharmacological studies. The phytochemical analysis was done after fractionating the ethanolic extract with n-hexane, chloroform, and hydro-alcohol. Then, the TLC method, DPPH radical scavenging, disc diffusion, membrane stabilization activity, heat-induced hemolysis, human blood clot lysis, and starch-iodine method were performed. The chemical investigation resulted in the identification of glycosides, amides, reducing sugars, flavonoids, phenols, alkaloids, tannins, saponins, gums, and steroids from the different extracts of the leaves of Ficus heteropleura Blume. In the antioxidant assay, hydro-alcoholic, chloroform, and n-hexane extracts at the dose of 100μg/ml produced significant inhibition of 76.11% (IC50 = 46.47 µg/ml), 72.83% (IC50 = 54.72 µg/ml), and 61.83% (IC50 = 77.95 µg/ml), respectively, as compared to the reference standard drug, ascorbic acid, 85.01% (IC50 = 32.22 µg/ml). However, only moderate anti-inflammatory activity was found in hydro-alcoholic extract (IC50 = 4.24 µg/ml). The extract also showed moderate thrombolytic and membrane-stabilizing effects. Hydro-alcoholic extract had antifungal activity against only Candida albicans (11 mm). However, chloroform plant extract exhibited significant antifungal activity against all included fungi. In the anti-diabetic study, significant inhibition of amylase activity was found by the hydro-alcoholic (73.33%) and chloroform (83.3%) extracts, respectively, whereas the n-hexane was found to have moderate activity (35.44%) compared to acarbose. Therefore, before isolation, characterization, and determination of the mechanism of action of the screened pharmacological activities, further investigation is needed.

https://doi.org/10.29169/1927-5951.2024.14.11
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References

Sharma B, RA. Updated review on Indian Ficus species. Arabian Journal of Chemistry 2023; 16(8): 104976. https://doi.org/10.1016/j.arabjc.2023.104976

Ghule ST, Patil DK. Development of medicinal plants. Kisan World 2001; 28: 33-4.

Frodin DG. History and concepts of big plant genera. Taxon 2004; 53(3): 753-76. https://doi.org/10.2307/4135449

Adebayo EA, Ishola OR, Taiwo OS, Majolagbe ON, Adekeye BT. Evaluations of the methanol extract of Ficus exasperata stem bark, leaf and root for phytochemical analysis and antimicrobial activities. African Journal of Plant Science 2009; 3(12): 283-7.

Chaudhary LB, Sudhakar JV, Kumar A, Bajpai O, Tiwari R, Murthy GV. Synopsis of the genus Ficus L.(Moraceae) in India. Taiwania 2012; 57(2): 193-216.

Mahajan MS, Gulecha VS, Khandare RA, Upaganlawar AB, Gangurde HH, Upasani CD. Anti-edematogenic and analgesic activities of Ficus benghalensis. International Journal of Nutrition, Pharmacology, Neurological Diseases 2012; 2(2): 100-4. https://doi.org/10.4103/2231-0738.95936

Patil VV, Patil VR. Evaluation of anti-inflammatory activity of Ficus carica Linn. leaves.

Thite AT, Patil RR, Naik SR. Anti-arthritic activity profile of methanolic extract of Ficus bengalensis: Comparison with some clinically effective drugs. Biomedicine & Aging Pathology 2014; 4(3): 207-17. https://doi.org/10.1016/j.biomag.2014.03.005

Ramakrishna V, Gupt KP, Setty OH, Kondapia AK. Protective effect of Ficus bengalensis L. extract against H2O2 induced DNA damage and repair in neuroblastoma cells. Free Radicals and Antioxidants 2014; 4(1): 3-7. https://doi.org/10.5530/fra.2014.1.2

Anjum NI, Tripathi YC. In vitro alpha-amylase and alpha-glucosidase inhibitory activities of fruits of Ficus auriculata Lour. Int J Pharma Bio Sci 2019; 10(4): 134-41. https://doi.org/10.22376/ijpbs.2019.10.4.p134-141

Kirtikar KR, Basu BD. International Book Distributors, Dehradun, India. Indian Medicinal Plant 1999; 3: 2262-3.

Khanom F, Kayahara H, Tadasa K. Superoxide-scavenging and prolyl endopeptidase inhibitory activities of Bangladeshi indigenous medicinal plants. Bioscience, Biotechnology, and Biochemistry 2000; 64(4): 837-40. https://doi.org/10.1271/bbb.64.837

Rastogi RM. Compendium of Indian medicinal plants. Central Drug Research Institute, Lucknow, India 1990; 1: 388-9.

Dhakite P, Arts SNM, Gadpayale J. Phytochemical Screening of Ficus heterophylla L.f., Leaf of Moraceae Family Research Journal of Pharmaceutical, Biological and Chemical Sciences Phytochemical Screening of Ficus heterophylla L.f., Leaf of Moraceae Family 2020.

Hoque M, Rahman MM, Uddin MN. A study of analgesic effect of medicinal plant Ficus heterophylla in Swiss albino mice. World Journal of Advanced Research and Reviews 2023; 19(3): 516-23. https://doi.org/10.30574/wjarr.2023.19.3.1804

Tabassum F, Chadni SH, Mou KN, Hasif KI, Ahmed T, Akter M. In-vitro thrombolytic activity and phytochemical evaluation of leaf extracts of four medicinal plants of Asteraceae family. Journal of Pharmacognosy and Phytochemistry 2017; 6(4): 1166-9.

Sieniawska E, Świątek Ł, Sinan KI, Zengin G, Boguszewska A, Polz-Dacewicz M, Bibi Sadeer N, Etienne OK, Mahomoodally MF. Phytochemical insights into Ficus sur extracts and their biological activity. Molecules 2022; 27(6): 1863. https://doi.org/10.3390/molecules27061863

Sirisha N, Sreenivasulu M, Sangeeta K, Chetty CM. Antioxidant properties of Ficus species-a review.

Omale J, Okafor PN. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. African Journal of Biotechnology 2008; 7(17).

Vadivu R, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchinensis (Lour) Moore ssp Laurina. Bangladesh Journal of Pharmacology 2008; 3(2): 121-4. https://doi.org/10.3329/bjp.v3i2.956

Shinde UA, Phadke AS, Nari AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Chemical and Biological Investigations of Different Partitionated Extracts of F.heteropleura Blume (Family: Moraceae) Leaves. Page 10 Membrane stabilization activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil 1999; 70: 251-257. https://doi.org/10.1016/S0367-326X(99)00030-1

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