Antioxidant, Cytotoxic, Membrane Stabilizing and Antimicrobial Activities of Bark and Seed of Entada phaseoloides (L.) Merr.: A Medicinal Plant from Chittagong Hill Tracts
DOI:
https://doi.org/10.6000/1927-5951.2011.01.02.13Keywords:
Antimicrobial, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC).Abstract
The methanolic crude extract of the bark and seed of Entada phaseoloides (L.) Merr. and its different organic soluble partitionates were screened for antioxidant, cytotoxic, membrane stabilizing and antimicrobial activities. The crude extract and carbon tetrachloride and aqueous soluble fractionsof both bark and seed showed higher level of total phenolic content (TPC, 245.59, 240.22, 240.03 & 117.0 mg of GAE/gm of dried extract). In the DPPH assay, the crude extract of bark and its chloroform and aqueous soluble fractions demonstrated strong antioxidant property with the IC50 of 3.24, 1.55 and 3.6 μg/ml, respectively whereas all the fractions of seed extract revealed mild antioxidant activity. The petroleum ether soluble fraction of both seed and bark exhibited significant cytotoxicity (LC50 = 1.54 & 5.4 μg/ml) which confers the presence of bioactive metabolites in this plant. On the other hand, the crude extract of seed and petroleum ether soluble fraction of bark inhibited the hemolysis of RBC of rat’s blood by 78.89 and 57.43%, respectively as compared to 84.44% exerted by acetyl salicylic acid (0.10 mg/ml). In antimicrobial screening, the carbon tetrachloride soluble fraction of bark showed significant antimicrobial activity against Staphylococcus aureus (zone of inhibition = 17.0 mm) with MIC and MBC values of 7.81 μg/ml and 125 μg/ml, respectively
References
Moermann DE. An analysis of the food plants and drug plants of native North America. J Ethnopharmacol 1996; 52: 1-22.
http://dx.doi.org/10.1016/0378-8741(96)01393-1
Srivastava J, Lambert J, Vietmeyer N. 1996. Medicinal plants: An expanding role in development. World Bank Technical; paper. No. 320.
Ghani A. Introduction to Pharmacognosy. Ahmadu Bello University Press, Ltd. Zaria, Nigeria; 1990; p. 45-47, 187-97.
Haroon AM. Effect of some macrophytes extracts on growth of Aspergillus parasiticus. Egyptain J Aqua Res 2006; 32: 301-13.
Ndubuisi JA, Emeka OE, Luke, UN. Physicochemical properties of chloroform extract of water hyacinth (Eichhornia crassipes). The African J Plant Sci Biotech 2007; 1: 40-2.
Roy S, Uddin MZ, Hassan MA, Rahman MM. Medico-botanical report on the Chakma community of Bangladesh. Bangladesh J Plant Taxon 2008; 15: 67-72.
Xiong H, Xiao E, Zhao Y, Yang G, Mei Z. Sulfur-containing amides from Entada phaseoloides. Acta Pharmaceutica Sinica 2010; 45: 624-6.
Ikegami F, Sekine T, Aburada M, Fujii Y, Komatsu Y, Murakoshi I. Synthesis of entadamide A and entadamide B isolated from Entada phaseoloides and their inhibitory effects on 5-lipoxygenase. Chem Pharm Bull (Tokyo). 1989; 37: 1932-3.
http://dx.doi.org/10.1248/cpb.37.1932
Ramakrishna, D., Pavan, KK., Mukkanti, K, Abedulla, KK. Antiulcer activity of the seeds of Entada phaseoloides. Pharmacologyonline 2008; 3: 93-9.
Jinrui D, Leonardus BSK, Soefjan T, Kosasih P, John MP, Kinghorn AD. Phenylacetic acid derivatives and a thioamide glycoside from Entada phaseoloides. Phytochemistr, 1991; 30: 3749-52.
http://dx.doi.org/10.1016/0031-9422(91)80102-7
Ikegami F, Sekine T, Duangteraprecha S, Matsushita N, Matsuda N, Ruangrungsi N. Entadamide C, a sulphur-containing amide from Entada phaseoloides. Phytochemistry 1989; 28: 881-2.
http://dx.doi.org/10.1016/0031-9422(89)80135-9
Perumal S, Klaus B, Harinder PS. Chemical composition, protein fractionation, essential amino acid potential and antimetabolic constituents of an unconventional legume, Gila bean (Entada phaseoloides Merrill) seed kernel. J Sci Food Agric 2002; 82: 192–202.
http://dx.doi.org/10.1002/jsfa.1025
Barua AK, Chakrabarty M, Datta PK, Ray S. Phaseoloidin, a homogentisic acid glucoside from Entada phaseoloides. Phytochemistry 1988; 27: 3259-61.
http://dx.doi.org/10.1016/0031-9422(88)80038-4
Sikder MAA, Siddique AB, Hossian AKMN, Miah MK, Kaisar MA, Rashid MA. Evaluation of thrombolytic activity of four Bangladeshi medicinal plants, as a possible renewable source for thrombolytic compounds. J Pharm Nutrition Sci 2011; 1: 4-8.
Kaisar MA, Rahman MS, Rahman MZ, Hasan CM, Rashid, MA. A review on phytochemicals from some medicinal plants
of Bangladesh. J Pharm Nutrition Sci 2011; 1: 87-95.
http://dx.doi.org/10.6000/1927-5951.2011.01.01.14
Van Wagenen BC, Larsen R, Cardellina JH, Ran dazzo D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri. J Org Chem 1993; 58: 335-7.
http://dx.doi.org/10.1021/jo00054a013
Skerget M, Kotnik P, Hadolin M, Hras A, Simonic M, Knez Z. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem 2005; 89: 191-8.
http://dx.doi.org/10.1016/j.foodchem.2004.02.025
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995; 28: 25-30.
http://dx.doi.org/10.1016/S0023-6438(95)80008-5
Choi HY, Jhun EJ, Lim BO, Chung IM, Kyung SH, Park DK. Application of flow injection-chemilumineacence to the study of radical scavenging activity in plants. Phytother Res 2000; 14: 250-3.
http://dx.doi.org/10.1002/1099-1573(200006)14:4<250:: AID-PTR587>3.0.CO;2-J
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen JB, Nicholsand DE, Mclaughlin JL. Brine shrimp; a convenient general bioassay for active plant constituents. Planta Med 1982; 45: 31-4.
http://dx.doi.org/10.1055/s-2007-971236
Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity - a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 1999; 70: 251-7.
http://dx.doi.org/10.1016/S0367-326X(99)00030-1
Bauer AW, Kirby WMM, Sherries JC, Tuck M. Antibiotic susceptibility testing by a standardized disc diffusion method. J Am Clin Pathol 1966; 45: 493-6.
Jennifer MA. Determination of minimum inhibitory concentrations. J Antimic Chemother 2001; 48: 5-16.
Turnidge JD, Ferraro MJ, Jorgensen JH. Manual of Clinical Microbiology. 8th Ed. Washington. American Society of Clinical Microbiology; 2003; p.1103.
Brumitt W, Hamilton-Miller MT, Gooding A. Importance of methodology in determining bactericidal and bacteriostatic activities of Azlocillin and ticarcillin against Pseudomonas aeruginosa. J Med Microbiol 1984; 17: 37- 44.
Downloads
Published
Issue
Section
License
Copyright (c) 2011 Fahima Aktar , Md. Ruhul Kuddus , Sk. Omar Faruque , Farhana Rumi , Mohiuddin Abdul Quadir , Mohammad A. Rashid
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Rashidi Othman, Suhair Kamoona, Irwandi Jaswir, Parveen Jamal , Farah Ayuni Mohd Hatta, Stability of Carotenoid Composition in Orange Sweet Potato (Ipomoea batatas) Tuber Flesh Over Three Growing Seasons and Months Storage Time , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 4 (2017)
- Naeem Quraishi, Tasneem Ahmad, Allah-Bux Ghanghro, Abdullah Arejo, Sharib S. Muhammad, Atta Chandio, Phase II Clinical Trial to Establish Efficacy of a Locally Appropriate Bivalent Anti Snake Venom in Pakistan , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
- Manoj Kumar Mishra, Dubey Deepti , Upamanyu Neeraj, Development and Evaluation of Controlled Release Bilayer Tablets of Hydrochlorothiazide and Losartan Potassium , Journal of Pharmacy and Nutrition Sciences : Vol. 5 No. 1 (2015)
- Rashidi Othman , Fatimah Azzahra Mohd Zaifuddin , Norazian Mohd Hassan, Characterisation of Carotenoid and Total Retinol Equivalent Content in Ulam and Medicinal Species as Alternative Food Intervention to Combat Vitamin A Deficiency , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
- Pakorn Suksangsri, Weerapat Santinakul, Wiwit Suttithumsatid, Pharkphoom Panichayupakaranant, Integrated Green Microwave Extraction and Hydrophobic Resin-Based Enrichment of Mitragynine from Mitragyna speciosa , Journal of Pharmacy and Nutrition Sciences : Vol. 16 (2026)
- Ken Yasukawa, Inhibitory Effect of a Combined Treatment of Glycyrrhizin and Caffeine on Tumor Promotion by 12-O-Tetradecanoylphorbol-13-Acetate in Two-Stage Carcinogenesis in Mouse Skin , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 3 (2013)
- Kana Sop Marie Modestine, Namadi Muhamadu, Tetanye Ekoe , Gouado Inocent, Effect of Spirulina platensis Supplementation on Nutritional and Biochemical Parameters of Under Five Years Malnourished Children from an Orphanage in Douala, Cameroon , Journal of Pharmacy and Nutrition Sciences : Vol. 5 No. 1 (2015)
- Md. Sahab Uddin , G.M. Sala Uddin , Mst. Marium Begum , Yesmin Begum , Oscar Herrera-Calderon , Md. Manjurul Islam , Mohamed M. Abdel-Daim, Inspection of Phytochemical Content and In Vitro Antioxidant Profile of Gnaphalium luteoalbum L.: An Unexplored Phytomedicine , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
- Sarah V. Cogle , Susan E. Smith , George O. Maish III , Gayle Minard , Martin A. Croce , Roland N. Dickerson, Sliding Scale Regular Human Insulin for Identifying Critically Ill Patients Who Require Intensive Insulin Therapy and for Glycemic Control in those with Mild to Moderate Hyperglycemia , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
- Azman Abdullah, Tan Chung Phern, Nur Fatihah Hanis Mohamad Rahim, Nur Izzati Zaharuddin, Nurdayana Maulat Salihin, Ahmad Hilmi Yusof, Kamsiah Jaarin, Yusof Kamisah, Qodriyah Haji Mohd Saad, Nur Azlina Mohd Fahami, Harizz Miszard Radman, The Effects of Cosmos caudatus (Ulam Raja) on the Levels of Expression of Nrf2 Target Genes in Mice Liver , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 4 (2017)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Mominur Rahman, Razibul Habib, Mohammed Aktar Sayeed, Muhammad Erfan Uddin, Rasheduzzaman Chowdhury, Mohammad A. Rashid, Amer Hasan , Mansoor Ahmed, Sedative and Anxiolytic Activities of Geodorum densiflorum Roots in Swiss Albino Mice , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 4 (2013)