Bioactivity of Pancratium Maritimum Methanolic Extract and its Alkaloid Fractions: Antimicrobial and Anti Α-Amylase Properties
DOI:
https://doi.org/10.29169/1927-5951.2026.16.07Keywords:
Pancratium maritimum, alkaloids, α-amylase inhibition, antibacterial, antifungalAbstract
Pancratium maritimum, or sea daffodil, is a species of bulbous plant that belongs to the Amaryllidaceae family, it is among one of the most important alkaloid-containing plant families. Methanolic extract of Pancartium maritimum was prepared. The phytochemicals in P. maritimum methanolic were identified using UHPLC-MS method. An in vitro spectrophotometric assay based on a colorimetric method was used to test the α- amylase inhibitory effect. Antibacterial and antifungal activities were tested against a wide range of relevant microorganisms using the broth dilution method.
This study showed the presence of alkaloids in methanolic extract as hordenine, lycorine and galanthamine, with an inhibitory action similar to acarbose at intermediate concentrations, the methanolic extract demonstrated significant concentration-dependent α-amylase inhibition, reaching 85.2% at 5 mg/mL (IC5₀ = 0.29 mg/mL). Alkaloid fraction displayed the highest antibacterial activity against the ATCC strain of Pseudomonas aeruginosa bacteria (MIC=1.125 mg/mL), butanolic fraction exerted a considerable anticandidal activity against Candida glabrata and candida parapsilosis fungi (MIC=0.031 and MIC=0.125mg/mL respectively).
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