Rose, a Potential Nutraceutical: An Assessment of the Total Phenolic Content and Antioxidant Activity
DOI:
https://doi.org/10.6000/1927-5951.2013.03.01.4Keywords:
Scavenging ability, solvent extraction, reducing power, Rosa hybrida L., total phenolic.Abstract
Rosa hybrida L. was reported to contain high total phenolic content and antioxidant activity. The scarce information on antioxidant properties of Malaysian cultivated R. hybrid L. had lead to the present study, which aimed to determine the effect of different solvent extraction on the total phenolic content and antioxidant activity of roses of different colours. All the 23 R. hybrida L. cultivars’ petals extracted with 70% ethanol had significantly higher 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity compared to the water extraction. The five cultivars (03, 203, 205, 402 and M203) that comprise the highest DPPH scavenging activity were subjected to various antioxidant assays. Cultivar M203 showed highest total phenolic content (TPC) at all concentration. Cultivar M203 and 402 gave higher DPPH radical scavenging ability (EC50=107.08 µg/ml) and 2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) radical cation scavenging ability (EC50=258.13 µg/ml). In ferric reducing antioxidant power assay, cultivar M203 has the highest trolox equivalent value at 200, 300 and 500 µg/ml concentrations while in b-carotene bleaching assay, cultivars 03, 205, and M203 (at the concentration of 500 µg/ml) showed higher antioxidant activity than synthetic antioxidant (BHA). Strong positive correlations were found between TPC and antioxidant activities, hence, suggesting that the high antioxidant activity of selected R. hybrida L. petals might be mainly contributed by the phenolic compounds. In general, cultivar M203 showed the best antioxidant activity with nutraceutical potential.
References
Percival M. Antioxidants. Clin Nutr Insight 1998; 31: 1-4.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem-Biol Interact 2006; 160: 1-40.
http://dx.doi.org/10.1016/j.cbi.2005.12.009
Halliwell B. Free radicals, antioxidants and human disease: Curiosity, cause or consequence? The Lancet 1994; 344: 721-4.
http://dx.doi.org/10.1016/S0140-6736(94)92211-X
Caillet S, Yu HL, Lessard S, Lamourex G, Ajdukovic D, Lacroix M. Fenton reaction applied for screening natural antioxidants. Food Chem 2005; 100: 542-52.
http://dx.doi.org/10.1016/j.foodchem.2005.10.009
Wong CC, Li HB, Cheng KW, Chen F. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 2006; 97: 705-11.
http://dx.doi.org/10.1016/j.foodchem.2005.05.049
Pisoschi AM, Cheregi MC, Danet AF. Total antioxidant capacity of some commercial fruit juices: Electrochemical and spectrophotometrical approaches. Molecules 2009; 14: 480-93.
http://dx.doi.org/10.3390/molecules14010480
Iris FF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: The FRAP assay. Anal Biochem 1996; 239: 70-76.
http://dx.doi.org/10.1006/abio.1996.0292
Leong LP, Shui G. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem 2002; 76: 69-75.
http://dx.doi.org/10.1016/S0308-8146(01)00251-5
Jacob JA, Mahal HS, Mukherjee T, Kapoor S. Free radical reactions with the extract of brassica family. Food Chem 2011; 129: 1132-8.
http://dx.doi.org/10.1016/j.foodchem.2011.05.091
Reidl KM, Hagerman EA. ABTS radical quenching and capacity by tannin-protein. J Agric Food Chem 2001; 49: 4917-23.
Antonio J, Meléndez M, Paul DF, Peter MB. Accumulation of health promoting phtochemicals in wild related and their contribution to in vitro antioxidant activity. Phytochem 2010; 71: 1104-14.
http://dx.doi.org/10.1016/j.phytochem.2010.03.021
Jaitak V, Sharma K, Kalia K, Kumar N, Singh HP, Kaul VK,
et al. Antioxidant activity of Potentilla fulgens: An alpine plant of western Himalaya. Food Compos Anal 2010; 23: 142-7.
http://dx.doi.org/10.1016/j.jfca.2009.02.013
Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S, Blanchi M, et al. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different In vitro assays. J Nutr 2003; 133: 2812-9.
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Food Compos Anal 2006; 19: 669-75.
http://dx.doi.org/10.1016/j.jfca.2006.01.003
Ee KY, Agboola S, Rehman A, Zhao J. Characterisation of phenolic compounds present in raw and roasted wattle (Acacia victoriae Bentham) seeds. Food Chem 2011; 129: 816-21.
http://dx.doi.org/10.1016/j.foodchem.2011.05.028
Wojdylo A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 2007; 105: 940-9.
http://dx.doi.org/10.1016/j.foodchem.2007.04.038
Ercisli S. Chemical composition of fruits in some rose (Rosa spp.) species. Food Chem 2007; 104: 1379-84.
http://dx.doi.org/10.1016/j.foodchem.2007.01.053
Choi EM, Hwang JK. Investigations of anti-inflammatory and antinociceptive activities of Piper cubeba, Physalic angulata and Rosa hybrida. J Ethnopharmacol 2003; 89: 171-5.
http://dx.doi.org/10.1016/S0378-8741(03)00280-0
Vinokur Y, Rodov V, Reznick N, Goldman G, Horev B, Umiel N, et al. Rose petal tea as an antioxidant-rich beverage: Cultivar effects. Food Sci 2006; 71: S42-S47.
http://dx.doi.org/10.1111/j.1365-2621.2006.tb12404.x
Lee JH, Lee HJ, Choung MG. 2011. Anthocyanin compositions and biological activities from the red petals of Korean edible rose (Rosa hybrid cv. Noblered). Food Chem 2011, 129, 272-8.
http://dx.doi.org/10.1016/j.foodchem.2011.04.040
Lim YY, Murtijaya J. Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT 2007; 40: 1664-9.
http://dx.doi.org/10.1016/j.lwt.2006.12.013
Zhang LZ, Jie GL, Zhang JJ, Zhao BL. Significant longevity- extending effects of EGCG on Canorhabditis elegans under stress. Free Radical Bio Med 2009; 46: 414-21.
http://dx.doi.org/10.1016/j.freeradbiomed.2008.10.041
Ordoñez AAL, Gomez JD, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 2005; 97: 452-8.
http://dx.doi.org/10.1016/j.foodchem.2005.05.024
Yu JM, Ahmedna M, Goktepe I. Effects of processing methods and extractions solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chem 2005; 90: 199-206.
http://dx.doi.org/10.1016/j.foodchem.2004.03.048
Zeng YW, Zhao JL, Peng YH. A comparative study on the free radical scavenging activities of some fresh flowers in southern China. LWT 2008; 41: 1586-91.
http://dx.doi.org/10.1016/j.lwt.2007.10.010
Cavaliere C. The effects of climate change on medicinal and aromatic plants. Herbalgram 2008; 81:44-57.
Zobayed SMA, Afreen F, Kozai T. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s wort. Plant Physiol Biochem 2005; 43: 977-84.
http://dx.doi.org/10.1016/j.plaphy.2005.07.013
Kirakosyan A, Seymour E, Kaufman PB, Warber S, Bolling S, Chang SC. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (hawthorn) subjected to drought and cold stress. J Agric Food Chem 2003; 51: 3973-6.
http://dx.doi.org/10.1021/jf030096r
Kalberer SR, Wisniewski M, Arora R. Deacclimation and reacclimation of cold hardy plants: Current understanding and emerging concepts. Plant Sci 2006; 171: 3-6.
http://dx.doi.org/10.1016/j.plantsci.2006.02.013
Mariod AA, Ibrahim RM, Ismail M, Ismail N. Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake. Food Chem 2009; 116; 306-12.
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Vui Fui Vun , Siong Chin Poh, Hip Seng Yim
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- L.V. Fedulova, Е.R. Vasilevskaya, Е.А. Kotenkova, A.A. Elkina, M.G. Baryshev , A.B. Lisitsyn, Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 2 (2017)
- Abubaker Ibrahim Elbur, Abdullah Salem AlMalki , Sultan Abdullah Alharthi, Self-Efficacy, Self-Care Activities Management and Treatment Outcome among Type 2 Diabetes Saudi Patients: Across-Sectional Study , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 2 (2017)
- D.A. Areshidze, L.D. Timchenko, M.A. Kozlova, I.A. Syomin, I.V. Rzhepakovsky, S.I. Piskov , V.N. Vakulin, Influence of Biologically Active Substance “STEMB” on a Morphofunctional Condition of a Liver and Kidneys of Rats at an Experimental Hepatorenal Syndrome , Journal of Pharmacy and Nutrition Sciences : Vol. 5 No. 3 (2015)
- Rama Mousleh, Shaza Al Laham, Histological Assessment of Pioglitazone Preventive Effect in Glycerol Contrast-Induced Nephropathy in Rats , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 2 (2017)
- Azman Abdullah, Tan Chung Phern, Nur Fatihah Hanis Mohamad Rahim, Nur Izzati Zaharuddin, Nurdayana Maulat Salihin, Ahmad Hilmi Yusof, Kamsiah Jaarin, Yusof Kamisah, Qodriyah Haji Mohd Saad, Nur Azlina Mohd Fahami, Harizz Miszard Radman, The Effects of Cosmos caudatus (Ulam Raja) on the Levels of Expression of Nrf2 Target Genes in Mice Liver , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 4 (2017)
- Befekadu Ejigu, Tesfaye Girma Legesse , Daniel Haile Chercos, Prevalence and Associated Factors of Malnutrition among Children Aged 6-59 Months in Addi Harush Eritrean Refugees Camp, Tigray Region, North Ethiopia , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 4 (2017)
- Viviana Loria-Kohen, Thais Lourenço-Nogueira, Isabel Espinosa-Salinas, Francisco R. Marín, Cristina Soler-Rivas , Ana Ramirez de Molina, Nutritional and Functional Properties of Edible Mushrooms: A Food with Promising Health Claims , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 3 (2014)
- Alejandro González Álvarez, Manuel Gómez Barrera, Joaquín Borrás Blasco , Emilio José Giner Serret, Adalimumab and Etanercept in the Treatment of Rheumatoid Arthritis and Spondyloarthropathies: Budget Impact Model of Dose Reduction , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 3 (2014)
- C. R. Vasudish, S. Nataraj , K. S. Premavalli, Development and Shelf Stability of Natural Fibre Rich Retort Pouch Ready to Eat Products , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 2 (2014)
- Seah Young Ng, Loo Wee Chia, Birdie Scott Padam , Fook Yee Chye, Effect of Selected Oligosaccharides on the Viability and Fermentation Kinetics of Lactobacillus acidophilus and Lactobacillus casei in Cultured Milk , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 2 (2014)
You may also start an advanced similarity search for this article.