Effects of Quercetin and Omega-3 Combination on Nuclear Factor Kappa B (NFκB) Expression Level in Pancreatic Tissue of Rats with Type-2 Diabetes Mellitus
DOI:
https://doi.org/10.6000/1927-5951.2017.07.01.1Keywords:
Quercetin, omega-3, type-2 diabetes mellitus, immunohistochemistry, nuclear factor kappa B (NFκB)Abstract
Background: Hyperglycemia increases nuclear factor kappa B (NFκB) expression and promotes cellular injury. Quercetin and omega-3 are expected to regulate NFκB expression. This study aims to measure the effect of combination therapy with quercetin and omega-3 in lowering the expression of NFκB in the pancreatic tissue of rats with type-2 DM as compared to those treated with monotherapy with either agent.
Methods: This experimental study involved the use of a paraffin block of pancreatic tissue from 24 male Wistar rats aged 3 months, weighing between 250 g and 350 g. All rats underwent induction of type-2 DM and were divided into 4 groups: K1 (treated daily with placebo), K2 (treated with quercetin at 20 mg·kgBW-1·d-1), K3 (treated with omega-3 at 100 mg·kgBW-1·d-1), and K4 (treated with quercetin at 20 mg·kgBW-1·d-1 and omega-3 at 100 mg·kgBW-1·d-1). Treatments were administered orally for four weeks. Once the treatment was completed, samples of pancreatic tissue were collected for the measurement of the percentage of NFκB expression using immunohistochemical (IHC) staining.
Results:The average level of NFκB expression in the pancreatic nuclei of DM rats treated with the combination of omega-3 and quercetin was significantly lower than that of those treated with placebo, quercetin only, or omega-3 only (p < 0.05).
Conclusion: The combination of quercetin at 20 mg·kgBW-1·d-1 and omega-3 at 100 mg·kgBW-1·d-1 is significantly more effective in lowering the percentage of NFκB in pancreatic nuclei than monotherapy with either agent.
References
World Health Organization. Global report on diabetes. Geneva; 2016: available on http://www.who.int.
Moussa S. Oxidative stress in diabetes mellitus. Rom J Biophys 2008; 18(3): 225-36.
Ayepola OR, Brooks NL, Oguntibeju OO. Oxidative stress and diabetic complications: the role of antioxidant vitamins and flavonoids. In: Antioxidant-antidiabetic agents and human health. Rijeka, Croatia: INTECH 2014.
Karasu C. Glycoxidative stress and cardiovascular complications in experimentally-induced diabetes: effects of antioxidant treatment. Open Cardiovasc Med J 2010; 4: 240-56. https://doi.org/10.2174/1874192401004010240
Erejuwa OO. Management of diabetes mellitus: could simultaneous targeting of hyperglycemia and oxidative stress be a better panacea? Int J Mol Sci 2012; 13(3): 2965-72. https://doi.org/10.3390/ijms13032965
Ingle PV, Talele GS. Adverse effects of metformin in combination with glimepiride and glibenclamide in patients with type 2 diabetes mellitus. Asian J Pharm Clin Res 2012; 5(Suppl 1): 108-110.
Cryer PE. Death during intensive glycemic therapy of diabetes: mechanisms and implications. Am J Med 2011; 124(11): 993-6. https://doi.org/10.1016/j.amjmed.2011.08.008
Kajimoto Y, Kaneto H. Role of oxidative stress in pancreatic beta-cell dysfunction. Ann N Y Acad Sci 2004; 1011: 168-76. https://doi.org/10.1196/annals.1293.017
Parabathina RK, Raja GV, Rao MN, Rao GS, Rao KS. Cardioprotective effects of vitamin E, morin, rutin and quercetin against doxorubicin induced oxidative stress of rabbits: a biochemical study. J Chem Pharm Res 2010; 2(3): 754-65.
Kelly GS. Quercetin. Monograph. Altern Med Rev 2011; 16(2): 172-94.
Anjaneyulu M, Chopra K. Quercetin, an anti-oxidant bioflavonoid, attenuates diabetic nephropathy in rats. Clin Exp Pharmacol Physiol 2004; 31(4): 244-8. https://doi.org/10.1111/j.1440-1681.2004.03982.x
Bardini G, Rotella CM, Giannini S. Dyslipidemia and diabetes: reciprocal impact of impaired lipid metabolism and beta-cell dysfunction on micro- and macrovascular complications. Rev Diabet Stud 2012; 9(2-3): 82-93. https://doi.org/10.1900/RDS.2012.9.82
Wardlaw G, Hampl J. Perspectives in nutrition 7th ed. New York: McGraw Hill 2007.
Fayez AM, Awad AS, El-Naa MM, Kenawy SA, El-Sayed ME. Beneficial effects of thymoquinone and omega-3 on intestinal ischemia/reperfusion-induced renal dysfunction in rats. Bull Fac Pharmacy, Cairo Univ 2014; 52(2): 171-7. https://doi.org/10.1016/j.bfopcu.2014.05.003
Barik R, Jain S, Qwatra D, Joshi A, Tripathi G, Goyal R. Antidiabetic activity of aqueous root extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats. Indian J Pharmacol 2008; 40(1): 19. https://doi.org/10.4103/0253-7613.40484
Pham NA, Schwock J, Iakovlev V, Pond G, Hedley DW, Tsao MS. Immunohistochemical analysis of changes in signaling pathway activation downstream of growth factor receptors in pancreatic duct cell carcinogenesis. BMC Cancer 2008; 8(1): 43. https://doi.org/10.1186/1471-2407-8-43
Starkey JM, Haidacher SJ, LeJeune WS, Zhang X, Tieu BC, Choudhary S, et al. Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Diabetes 2006; 55(5): 1252-9. https://doi.org/10.2337/db05-1554
Bischoff SC. Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care 2008; 11(6): 733-40. https://doi.org/10.1097/MCO.0b013e32831394b8
Indra M, Karyono S, Ratnawati R, Malik SG. Quercetin suppresses inflammation by reducing ERK1/2 phosphorylation and NF kappa B activation in leptin-induced human umbilical vein endothelial cells (HUVECs). BMC Res Notes 2013; 6(1): 275. https://doi.org/10.1186/1756-0500-6-275
Zwart SR, Pierson D, Mehta S, Gonda S, Smith SM. Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-?B activation: from cells to bed rest to astronauts. J Bone Miner Res 2009; 25(5): 1049-57. https://doi.org/10.1359/jbmr.091041
Vanden BW, Vermeulen L, Delerive P, De Bosscher K, Staels B, Haegeman G. A paradigm for gene regulation: inflammation, NF-kappaB and PPAR. Adv Exp Med Biol 2003; 544: 181-96. https://doi.org/10.1007/978-1-4419-9072-3_22
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