logo

In Vitro Membrane Stabilizing and Thrombolytic Activities of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflora Growing in Bangladesh

Authors

  • Farhana Islam

    Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

  • Sharmin Reza Chowdhury

    Department of Pharmacy, State University of Bangladesh, Dhaka-1205, Bangladesh

  • Tasnuva Sharmin

    Department of Pharmacy, State University of Bangladesh, Dhaka-1205, Bangladesh

  • Md. Gias Uddin

    Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

  • Mohammad A. Kaisar

    Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

  • Mohammad A. Rashid

    Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.6000/1927-5951.2013.03.01.8

Keywords:

 Hypotonic solution, haemolysis, acetyl salicylic acid, streptokinase, clot lysis.

Abstract

The methanol extracts and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflorawere subjected to assays for membrane stabilizing and thrombolytic activities. The extractives inhibited heat- as well as hypotonic solution-induced haemolysis of human erythrocytes in vitro. The pet-ether soluble fraction of O. mungos, M. macrophylla and S. nodiflora demonstrated 61.16 % & 24.75%, 52.55% & 23.35% and 60.24% & 22.85% inhibition of hemolysis of RBC caused by hypotonic solution and heat, whereas the carbon tetrachloride soluble fraction of G. philippensis showed 49.05% and 21.25% inhibition of hypotonic and heat induced hemolysis of RBC, respectively. Here, acetyl salicylic acid was used as reference standard at 0.10 mg/mL. Among the four plants, the carbon tetrachloride soluble fraction of O. mungos, methanol extract of M. macrophylla, carbon tetrachloride soluble fraction of G. philippensis and chloroform soluble fraction of S. nodiflora revealed highest thrombolytic activity with clot lysis value of 50.09%, 49.50%, 47.14% and 46.37%, respectively. Standard streptokinase and water were used as positive and negative controls which showed 65.00% and 3.84% lysis of clot, respectively.

References

Ayyanar M, Ignacimuthu S. Herbal medicines for wound healing among tribal people in Southern India: Ethnobotanical and Scientific evidences. Intl J Appl Res Nat Prod 2009; 2: 29-42.

Peter JH, Ibironke MO. Flowering plants used against snakebite. J Ethnopharmacol 1993; 39: 1-29.

http://dx.doi.org/10.1016/0378-8741(93)90047-9

Manandar NP, Manandar SP. Plants and People of Nepal, Timber Press 2002; p. 327.

Anonymous. Dictionary of Chinese Traditional Medicine, Jiangsu New Medical College, Shanghai Science and Technology Press 1986; p. 176.

Dinda B, Chowdhury DR, Monhata BC. Naturally occurring iridoids, secoiridoids and their bioactivity. An update review, part 3. Chem Pharm Bull 2009; 57: 765-96.

http://dx.doi.org/10.1248/cpb.57.765

Kim NC, Desjardins AE, Wu CD. Activity of triterpenoid glycosides from the root barks of Mussaenda macrophylla against oral pathogens. J Nat Prods 1999; 62: 1379-84.

http://dx.doi.org/10.1021/np9901579

Helfrich E, Rimpler. Iriod glycosides from Gmelina phillippensis. Phytochemistry 2000; 54: 191-99.

http://dx.doi.org/10.1016/S0031-9422(00)00060-1

Chung RCK. Gmelina philippensis Cham. In: van Valkenburg, JLCH. and Bunyapraphatsara, N. (Editors). Plant Resources of South-East Asia No. 12(2): Medicinal and poisonous plants 2. Backhuys Publisher, Leiden, The Netherlands 2001; p. 281.

Watson L, Dallwitz M. The families of flowering plants: Description, Illustration, Identification and Information retrieval. DELTA -taxonomic data bases 2000.

Forsetieri AM, Monforte MT, Ragusa S, Trovato A, Lauk L. Antiinflammatory, analgesic and antipyretic activity in rodents of plant extracts used in African medicine. Phytother Res 1996; 10: 100-106.

http://dx.doi.org/10.1002/(SICI)1099-1573(199603)10:2<100::AID-PTR724>3.0.CO;2-I

Abada KA. Chemical control of sugar beet damping-off and root-rot diseases. Egypt J Phytopathol 1995; 33: 1-8.

Belmain SR, Nealb GE, Rayb DE, Goloba P. Insecticidal and vertebrate toxicity associated with ethnobotanicals used as post-harvest protectants in Ghana. Food Chem Toxicol 2001; 39: 287-91.

http://dx.doi.org/10.1016/S0278-6915(00)00134-4

Prekeyi TF, Omonigho O. Effects of dietary supplementation of node weed (Synedrella nodiflora) on toxicity of copper and lead in guinea pigs Cavia porcellus. Toxicol Environ Chem 2007; 89: 215-22.

http://dx.doi.org/10.1080/02772240601019999

Kaisar MA, Rahman MS, Rahman MZ, Hasan CM, Rashid MA. A review on phytochemicals from some medicinal plants of Bangladesh. J Phar Nutri Sci 2011; 1: 87-95.

http://dx.doi.org/10.6000/1927-5951.2011.01.01.14

Sharmin T, Islam F, Kaisar MA, Uddin MG, Rashid MA. Antioxidant, Thrombolytic and Cytotoxic Activities of Picrasma javanica. Dhaka Univ J Pharm Sci 2012; 11: 71-74.

http://dx.doi.org/10.3329/dujps.v11i1.12491

Van Wagenen BC, Larsen R, Cardellina JH, Ran dazzo D, Lidert ZC, Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosaruetzleri. J Org Chem 1993; 58: 335-37.

Shindhe UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity– a possible mechanism of action for the anti-inflammatory activity of Cedrusdeodara wood oil. Fitoterapia 1999; 70: 251-57.

http://dx.doi.org/10.1016/S0367-326X(99)00030-1

Sikder MA, Rahman MA, Kaisar MA, Rahman MS, Hasan CM, Rashid MA. In vitro Antioxidant, Reducing Power, Free Radical Scavenging and Membrane stabilizing activities of seeds of Syzygium cumini L. Lat Am J Pharm 2011; 30: 781-85.

Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. Development of invitro model to study clot lysis activity of thrombolytic drugs. Thromb J 2006; 4: 14.

http://dx.doi.org/10.1186/1477-9560-4-14

Omale J, Okafor PN. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. Afr J Biotechnol 2008; 7: 3129-33.

Abe H, Katada K, Orita M, Nishikibe M. Effects of calcium antagonists on the erythrocyte membrane. J Pharm Pharmacol 1991; 43: 22-26.

http://dx.doi.org/10.1111/j.2042-7158.1991.tb05441.x

Downloads

Published

2013-01-05

Issue

Vol. 3 No. 1 (2013)

Section

Articles

License

Copyright (c) 2013 Mohammad A. Rashid, Mohammad A. Kaisar, Md. Gias Uddin, Tasnuva Sharmin, Sharmin Reza Chowdhury, Farhana Islam

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

In Vitro Membrane Stabilizing and Thrombolytic Activities of Ophirrhiza mungos, Mussaenda macrophylla, Gmelina philippensis and Synedrella nodiflora Growing in Bangladesh . (2013). Journal of Pharmacy and Nutrition Sciences , 3(1), 71-75. https://doi.org/10.6000/1927-5951.2013.03.01.8

Similar Articles

71-80 of 99

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)