Lycopene Potentiates the Protective Effect of Aliskiren on Doxorubicin-Induced Cardiomyopathy in Rats
DOI:
https://doi.org/10.6000/1927-5951.2015.05.03.5Keywords:
Aliskiren, lycopene, doxorubicin, cardiomyopathy, antioxidant enzymes.Abstract
Objective: The present study was designed to explore the combination therapy of lycopene with aliskiren in doxorubicin induced cardiomyopathy.
Methods: Cardiomyopathy was induced in Wistar rats by i.p. administration of Doxorubicin (DOX) (15 mg/kg, single dose). Haemodynamic parameters (Systolic, diastolic blood pressure, Heart rate), heart weight, heart weight/body weight ratio, serum lactate dehydrogenase (LDH), oxidative stress (TBARS) and antioxidant enzymes (Glutathione, SOD and catalase) as well as transmission electron microscopein heart tissue were carried out.
Results: There was significant increase in blood pressure, serum, lactate dehydrogenase (LDH), oxidative stress (TBARS) and significant decrease in heart weight, heart weight/body weight ratio and antioxidant enzymes in cardiomyopathic rats. Combined therapy of lycopene (2 and 4mg/kg) with aliskiren (100 mg/kg) treatment showed pronounced beneficial effecton above parameters.Furthermore lycopene with aliskiren significantly improves the antioxidant defense by increasing reduced glutathione, SOD, catalase, heart size and heart weight/body weight ratio. It is clearly observed from Transmission electron microscopic slides of DOX treated rats, there was swelling of mitochondria with disruption of cristae, rupture of nuclear membrane, condensation and margination of nuclear chromatins which were well protected by lycopene along with aliskiren treatment.
Conclusion: Therefore, combination therapy of lycopene with aliskiren offers better treatment for DOX-induced cardiomyopathy than ALK alone.
References
Sacco G, et al. Cardioprotective effects of zofenopril, a new angiotensin-converting enzyme inhibitor, on doxorubicin-induced cardiotoxicity in the rat. European Journal of Pharmacology 2001; 414: 71-78. http://dx.doi.org/10.1016/S0014-2999(01)00782-8
Luca G, et al. Anthracycline cardiomyopathy in breast cancer patients: synergism with trastuzumab and taxanes. Cardiovascular Toxicology 2007; 7: 67-71. http://dx.doi.org/10.1007/s12012-007-0013-5
Hiona A, et al. Pre-treatment with ACE inhibitor attenuates doxorubicin-induced cardiomyopathy via preservation of mitochondrial function. J Thorac Cardiovac Surg 2011; 142: 396-403. http://dx.doi.org/10.1016/j.jtcvs.2010.07.097
Siveski-Iliskovic N, et al. Probucol promotes endogenous antioxidants and provides protection against adriamycin-induced cardiomyopathy in rats. Circulation 1994; 89: 2829-2835. http://dx.doi.org/10.1161/01.CIR.89.6.2829
Sharma H, et al. Anti-apoptotic potential of rosuvastatin pretreatment in murine model of cardiomyopathy. Int J Cardiol 2011; 150: 193-200. http://dx.doi.org/10.1016/j.ijcard.2010.04.008
Ferreira AL, et al. Doxorubicin as an antioxidant: maintenance of myocardial levels of lycopene under doxorubicin treatment. Free Radic Biol Med 2007; 43: 740-51. http://dx.doi.org/10.1016/j.freeradbiomed.2007.05.002
Myers CE, et al. Adriamycin: the role of lipid peroxidation in cardiac toxicity and tumor response. Science 1977; 197: 165-167. http://dx.doi.org/10.1126/science.877547
Rashikh A, et al. Aliskiren attenuates myocardial apoptosis and oxidative stress in chronic murine model of cardiomyopathy. Biomed Pharmacother 2012; 5: 529-535. http://dx.doi.org/10.1016/j.biopha.2011.11.020
Toko H, et al. Angiotensin II: type 1a receptor mediates Doxorubicin-induced cardiomyopathy. Hypertens Res 2002; 25: 597-603. http://dx.doi.org/10.1291/hypres.25.597
Nazeyrollas P, et al. Effects of amifostine on perfused isolated rat heart and on acute doxorubicin-induced cardiomyopathy. Cancer Chemother Pharmacol 1999; 43: 227-32. http://dx.doi.org/10.1007/s002800050888
Liu X, et al. Melatonin as an effective protector against doxorubicin-induced cardiomyopathy. Am J Physiol: Heart Cir Physiol 2002; 283: H254-63. http://dx.doi.org/10.1152/ajpheart.01023.2001
Xu Z, et al. Ghrelin prevents doxorubicin-induced cardiotoxicity through TNF-alpha/NF-kB pathways and mitochondrial protective mechanisms. Toxicol 2008; 247: 133-138. http://dx.doi.org/10.1016/j.tox.2008.02.018
Soga M, et al. Effects of angiotensin II receptor blocker in daunorubicin-induced cardiomyopathic rats. Intern J Cardiol 2006; 110: 378-385. http://dx.doi.org/10.1016/j.ijcard.2005.08.061
Hollenberg NK, et al. Pathways for angiotensin II generation in intact human tissue: evidence from comparative pharmacological interruption of the renin system. Hypertens 1998; 32: 387-92. http://dx.doi.org/10.1161/01.HYP.32.3.387
Young DS. Effects of drugs on clinical laboratory tests. Washington: AACC press, 1990; 120-22.
Stahl W, Sies H. Physical quenching of singlet oxygen and cis-trans isomerization of carotenoids. Annals New York Academy of Science 1993; 691: 10-19. http://dx.doi.org/10.1111/j.1749-6632.1993.tb26153.x
Di Mascio P, et al. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 1989; 274: 532-538. http://dx.doi.org/10.1016/0003-9861(89)90467-0
Ojha S, et al. Cardioprotective effect of lycopene against isoproterenol-induced myocardial infarction in rats. Human and Experimental Toxicology 2013; 32: 492-503. http://dx.doi.org/10.1177/0960327112454890
Pennathur S, et al. Potent antioxidative activity of lycopene: A potential role in scavenging hypochlorous acid. Free Radic Biol Med 2010; 49(2): 205-13. http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.003
Ferreira AL, et al. Tomato-oleoresin supplement prevents doxorubicin-induced cardiac myocyte oxidative DNA damage in rats. Mutat Res 2007; 631: 26-35. http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.003
Ohkawa H, et al. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 359-64. http://dx.doi.org/10.1016/0003-2697(79)90738-3
Sedlack J, Lindsay RH. Estimation of total, protein bound and non-protein bound sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968; 25: 192-205. http://dx.doi.org/10.1016/0003-2697(68)90092-4
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47: 469-74. http://dx.doi.org/10.1111/j.1432-1033.1974.tb03714.x
Clairbome A. Assay of catalase. In: Greenwald RA, editor. Handbook of Methods of Oxygen Free Radical Research. Boca Raton: CRC Press, 1985; 283-84.
Singal PK, Iliskovic N. Adriamycin cardiomyopathy. N Engl J Med 1998; 339: 900-905. http://dx.doi.org/10.1056/NEJM199809243391307
Pathan RA, et al. Naproxen aggravates Doxorubicininduced cardiomyopathy in rats. Indian J Pharmacol 2010; 42: 44-49. http://dx.doi.org/10.4103/0253-7613.62411
Zhu W, et al. MAPK superfamily plays an important role in daunomycin-induced apoptosis of cardiac myocytes. Circ 1999; 100: 2100-07. http://dx.doi.org/10.1161/01.CIR.100.20.2100
Goto T, Takase H, Toriyama T. Circulating concentrations of cardiac proteins indicate the severity of congestive heart failure. Heart 2003; 89: 1303-7. http://dx.doi.org/10.1136/heart.89.11.1303
Singh BK, et al. Diclofenac sodium, a nonselective nonsteroidal anti-inflammatory drug aggravates doxorubicin-induced cardiomyopathy in rats. J Cardiovascul Pharmacol 2010; 55; 139-14. http://dx.doi.org/10.1097/FJC.0b013e3181c87e17
Yilmaz S, et al. Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology 2006; 218: 164-171. http://dx.doi.org/10.1016/j.tox.2005.10.015
Downloads
Published
Issue
Section
License
Copyright (c) 2015 Vinay Kumar, Surama Chauhan, K. Nagarajan , Bhulan Kumar Singh
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Sufi Desrini , I.M. Kadek Dwi, Antihyperglicemic and Antioxidant Potential of Aloe vera Juice Supplementation in the Type-II Diabetic Rats Model , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 2 (2018)
- Teresa Adamska, Małgorzata Ewertowska, Ewa Ignatowicz, Małgorzata Kujawska, Marcin Kidoń, Arleta Matuszewska, Jan Oszmiański, Hanna Szaefer, Jadwiga Jodynis-Liebert, Effects of Long-Term Administration of Freeze-Dried Chokeberry Juice to Rats , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 2 (2014)
- Alexander A. Kravtsov , Stanislav V. Kozin , Ekaterina R. Vasilevskaya , Anna A. Elkina , Lilia V. Fedulova , Konstantin A. Popov , Vadim V. Malyshko , Arkady V. Moiseev , Denis I. Shashkov , Mikhail G. Baryshev, Effect of Drinking Ration with Reduced Deuterium Content on Brain Tissue Prooxidant-Antioxidant Balance in Rats with Acute Hypoxia Model , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 2 (2018)
- Eman Aly Sadeek Fadlalla, Cardio-Nephroprotective Effects of Guava and Olive Leaves Extracts on Doxorubicin-Induced Toxicity in Rats , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 3 (2018)
- Azman Abdullah, Tan Chung Phern, Nur Fatihah Hanis Mohamad Rahim, Nur Izzati Zaharuddin, Nurdayana Maulat Salihin, Ahmad Hilmi Yusof, Kamsiah Jaarin, Yusof Kamisah, Qodriyah Haji Mohd Saad, Nur Azlina Mohd Fahami, Harizz Miszard Radman, The Effects of Cosmos caudatus (Ulam Raja) on the Levels of Expression of Nrf2 Target Genes in Mice Liver , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 4 (2017)
- Rogério Corrêa Peres, Rafael Hirata, Samuel Rangel Claudio, Taina Liz, Tamires Corrêa da Silva , Daniel Araki Ribeiro, Red Dry Wine Modulates Plasma Cholesterol Levels and Cardiac Enzymes in Humans , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 1 (2012)
- Vui Fui Vun, Siong Chin Poh, Hip Seng Yim, Rose, a Potential Nutraceutical: An Assessment of the Total Phenolic Content and Antioxidant Activity , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 1 (2013)
- Sousan Kolahi, Amir Ghorbanihaghjo, Ali-Reza Khabbazi, Mehrzad Hajialilo , Hale Farzin, The Effect of Fish Oil Supplement on Serum Antioxidant Level in Patient with Rheumatoid Arthritis , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 4 (2013)
- Manisha Bhatti, Anjoo Kamboj , Ajay Kumar Saluja, Phytochemical Screening and In-Vitro Evaluation of Antioxidant Activities of Various Extracts of Leaves and Stems of Kalanchoe crenata , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 1 (2012)
- Md. Sahab Uddin , Md. Sarwar Hossain , Md. Tanvir Kabir , Imon Rahman , Devesh Tewari , Mohd. Raeed Jamiruddin , Abdullah Al Mamun, Phytochemical Screening and Antioxidant Profile of Syngonium podophyllum Schott Stems: A Fecund Phytopharmakon , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 3 (2018)
You may also start an advanced similarity search for this article.