A Green Method for Preparation of Cyanidin-3-glucoside from Carissa carandas Fruits and α-Glucosidase Inhibitory Activity Evaluation
DOI:
https://doi.org/10.29169/1927-5951.2024.14.10Keywords:
Antidiabetic, α- Glucosidase, Enriched extract, Green extraction, Carissa carandas, Cyanidin-3-glucosideAbstract
In this research, a tailored approach for the preparation of a cyanidin-3-glucoside-enriched extract (C3GE) and cyanidin-3-glucoside (C3G) from the fruits of Carissa carandas L. was achieved using a green methodology. The method involved a cold extraction, followed by fractionation processes on a hydrophobic (Diaion® HP-20) column using a hydroethanolic solvent system for column elution. C3GE was produced after the one-step fractionation, while C3G was obtained after the two-step fractionation. Based on an HPLC method, C3GE contained 34.8% w/w of C3G, while C3G was identified via its 1H and 13C NMR data. An in vitro assay for the α-glucosidase inhibitory effect revealed that C3GE and C3G possessed good inhibitory activity against α-glucosidase, with IC50 values of 19.7 and 4.4 µg/mL, respectively, which is better than that of acarbose (IC50 of 395.4 µg/mL). Our findings suggest the potential use of this green extraction method for the production of C3G and C3GE, as well as its application in functional ingredient industries, including nutraceuticals and pharmaceuticals.
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