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Oxidative Stress Promotes Eating Behavior and Obesity in C. elegans via EGL-4 / DAF-16 Signaling

Authors

  • Wensheng Li

    Marshall University, Huntington, WV 25755, USA

  • Eric Blough

    Marshall University, Huntington, WV 25755, USA

  • Cuifen Wang

    Marshall University, Huntington, WV 25755, USA

  • Larry Dial

    Marshall University, Huntington, WV 25755, USA

  • Omolola Olajide

    Marshall University, Huntington, WV 25755, USA

  • Randa Al-Jayoussi

    Marshall University, Huntington, WV 25755, USA

  • Nesreen BenHamed

    Marshall University, Huntington, WV 25755, USA

  • Miaozong Wu

    Marshall University, Huntington, WV 25755, USA

DOI:

https://doi.org/10.6000/1927-5951.2015.05.02.5

Keywords:

 Oxidative stress, Eating behavior, Obesity, EGL4/cGMP-dependent protein kinases, DAF-16/FOXO, C. elegans, HEK293 cells.

Abstract

Oxidative stress is associated with pathophysiological progress of many diseases. The objective of study was to investigate whether increased environmental oxidative stress stimulation can promote excessive eating behavior, a common cause of obesity, and to identify the molecular mechanism.The cGMP-dependent kinase (PKG) activator 8-pCPT-cGMP was applied in worm swimming assay to study behavior shifting between quiescence and foraging in C. elegans. Genetically modified C. elegans (egl-4 loss or gain of function, and daf-16 mutant) were treated with paraquat, an oxidative stress inducer. Worm’s foraging behavior, body fat accumulation and body length were determined. The foxo1::gfp-transfectedHEK293 cells and C. elegans (daf-16::gfpTJ356) were further used to examine the effect of paraquat on PKG expression and FOXO nuclear translocation.A novel swimming assay using PKGactivator stimulation was developed, which allows the rapid and effective study of foraging behavior in C. elegans. Paraquat treatment significantly inhibited quiescence, promoted foraging behavior, increased body fat accumulation and body growth. These responses were associated with diminished PKG expression/activation and increased FOXO (DAF-16) nuclear translocation in both transfected C. elegans and HEK293 cells.Our data suggest that PKG/FOXO signaling may plays an important role in mediating oxidative stress-induced excessive eating behavior and obesity development.

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Published

2015-04-05

Issue

Vol. 5 No. 2 (2015)

Section

Articles

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Copyright (c) 2015 Wensheng Li, Eric Blough, Cuifen Wang, Larry Dial, Omolola Olajide, Randa Al-Jayoussi, Nesreen BenHamed , Miaozong Wu

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How to Cite

Oxidative Stress Promotes Eating Behavior and Obesity in C. elegans via EGL-4 / DAF-16 Signaling. (2015). Journal of Pharmacy and Nutrition Sciences , 5(2), 129-136. https://doi.org/10.6000/1927-5951.2015.05.02.5

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