A Green Method for Preparation of Cyanidin-3-glucoside from Carissa carandas Fruits and α-Glucosidase Inhibitory Activity Evaluation
DOI:
https://doi.org/10.29169/1927-5951.2024.14.10Keywords:
Antidiabetic, α- Glucosidase, Enriched extract, Green extraction, Carissa carandas, Cyanidin-3-glucosideAbstract
In this research, a tailored approach for the preparation of a cyanidin-3-glucoside-enriched extract (C3GE) and cyanidin-3-glucoside (C3G) from the fruits of Carissa carandas L. was achieved using a green methodology. The method involved a cold extraction, followed by fractionation processes on a hydrophobic (Diaion® HP-20) column using a hydroethanolic solvent system for column elution. C3GE was produced after the one-step fractionation, while C3G was obtained after the two-step fractionation. Based on an HPLC method, C3GE contained 34.8% w/w of C3G, while C3G was identified via its 1H and 13C NMR data. An in vitro assay for the α-glucosidase inhibitory effect revealed that C3GE and C3G possessed good inhibitory activity against α-glucosidase, with IC50 values of 19.7 and 4.4 µg/mL, respectively, which is better than that of acarbose (IC50 of 395.4 µg/mL). Our findings suggest the potential use of this green extraction method for the production of C3G and C3GE, as well as its application in functional ingredient industries, including nutraceuticals and pharmaceuticals.
References
Anupama N, Madhumitha G, Rajesh KS. Role of dried fruits of Carissa carandas as anti-inflammatory agents and the analysis of phytochemical constituents by GC-MS. BioMed Research International 2014; 2014: 512369. https://doi.org/10.1155/2014/512369
Dhodi JB, Thanekar DR, Mestry SN, Juvekar AR. Carissa carandas Linn. fruit extract ameliorates gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress. J Acute Dis 2015; 4: 135-140. https://doi.org/10.1016/S2221-6189(15)30023-8
Singh R, Shrivastava M, Sharma P. Antidiabetic effect of Carissa carandas in rats and the possible mechanism of its insulin secretagogues activity in isolated pancreatic islets. J. Biomed Ther Sci 2019; 6: 1-7.
Lailerd M, Linn TW, Lailerd N, Amornlerdpison D, Imsumran A. Assessment of antidiabetic and anti-inflammatory activities of Carissa carandas Linn extract: In vitro and in vivo study. Appl Sci 2023; 13(11): 6454. https://doi.org/10.3390/app13116454
Mishra AB, Tomer V, Kumar A. Karonda (Carissa carandas L.): A miracle fruit with multifaceted potential. J Agric Food Res 2024; 18: 101417. https://doi.org/10.1016/j.jafr.2024.101417
Olivas-Aguirre FJ, Rodrigo-García J, Martínez-Ruiz ND, Cárdenas-Robles AI, Mendoza-Díaz SO, Álvarez-Parrilla E, González-Aguilar GA, de la Rosa LA, Ramos-Jiménez A, Wall-Medrano A. Cyanidin-3-O-glucoside: Physical-chemistry, foodomics and health effects. Molecules 2016; 21(9): 1264. https://doi.org/10.3390/molecules21091264
Cásedas G, Les F, González-Burgos E, Gómez-Serranillos MP, Smith C, López V. Cyanidin-3-O-glucoside inhibits different enzymes involved in central nervous system pathologies and type-2 diabetes. S Afr J Bot 2019; 120: 241-246. https://doi.org/10.1016/j.sajb.2018.07.001
Pan J, Zhang Q, Zhang C, Yang W, Liu H, Lv Z, Liu J, Jiao Z. Inhibition of dipeptidyl peptidase-4 by flavonoids: structure-activity relationship, kinetics and interaction mechanism. Front Nutr 2022; 9: 892426. https://doi.org/10.3389/fnut.2022.892426
Bartel I, Koszarska M, Strzałkowska N, Tzvetkov NT, Wang D, Horbańczuk JO, Wierzbicka A, Atanasov AG, Jóźwik A. Cyanidin-3-O-glucoside as a nutrigenomic factor in type 2 diabetes and its prominent impact on health. Int J Mol Sci 2023; 24(11): 9765. https://doi.org/10.3390/ijms24119765
Ye X, Chen W, Huang XF, Yan FJ, Deng SG, Zheng XD, Shan PF. Anti-diabetic effect of anthocyanin cyanidin-3-O-glucoside: data from insulin resistant hepatocyte and diabetic mouse. Nutr Diabetes 2024; 14(1): 1-8. https://doi.org/10.1038/s41387-024-00265-7
Chen L, Xin X, Lan R, Yuan Q, Wang X, Li Y. Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography. Food Chem 2014; 152: 386-90. https://doi.org/10.1016/j.foodchem.2013.11.080
Lee JS, Kim YR, Song IG, Ha SJ, Kim YE, Baek NI, Hong EK. Cyanidin-3-glucoside isolated from mulberry fruit protects pancreatic β-cells against oxidative stress-induced apoptosis. Int J Mol Med 2015; 35(2): 405-412. https://doi.org/10.3892/ijmm.2014.2013
Sasikumar R, Jyoti Das A, Chandra Deka S. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi J Biol Sci 2021; 28(9): 5338-5348. https://doi.org/10.1016/j.sjbs.2021.05.065
Xu Z, Liu W, Zhu L, Sun S, Xin X, Chen L, Liu Y. Preparative isolation and purification of three anthocyanins from red raspberry (Rubus ideaus L.) fruits by high-speed counter-current chromatography based on the optimization of the ultrasound-assisted extraction method. ACS Food Sci Technol 2022; 2(11): 1710-1718. https://doi.org/10.1021/acsfoodscitech.2c00215
Suttithumsatid W, Shah MA, Bibi S, Panichayupakaranant P. α-Glucosidase inhibitory activity of cannabidiol, tetrahydrocannabinol and standardized cannabinoid extracts from Cannabis sativa. Curr Res Food Sci 2022; 5: 1091-1097. https://doi.org/10.1016/j.crfs.2022.07.002
Oancea S. A Review of the current knowledge of thermal stability of anthocyanins and approaches to their stabilization to heat. Antioxidants (Basel) 2021; 10(9): 1337. https://doi.org/10.3390/antiox10091337
Panichayupakaranant, P. Active constituent-rich herbal extracts for development of phytomedicine. Songklanagarind Med J 2017; 35(3): 187-193. https://doi.org/10.31584/smj.2017.35.3.728
Shah MA, Reanmongkol W, Radenahmad N, Khalil R, Ul-Haq Z, Panichayupakaranant P. Anti-hyperglycemic and anti-hyperlipidemic effects of rhinacanthins-rich extract from Rhinacanthus nasutus leaves in nicotinamide-streptozotocin induced diabetic rats. Biomed Pharmacother 2019; 113: 108702. https://doi.org/10.1016/j.biopha.2019.108702
Meah MS, Panichayupakaranant P. α-Mangostin-rich extract: A potential oral antibacterial agent prepared by green extraction. Int J Pharmacogn Chinese Med 2020; 4: 000201. https://doi.org/10.23880/ipcm-16000201
Bhuyan P, Ganguly M, Baruah I, Borgohain G, Hazarika J, Sarma S. Alpha glucosidase inhibitory properties of a few bioactive compounds isolated from black rice bran: combined in vitro and in silico evidence supporting the antidiabetic effect of black rice. RSC Adv 2022; 12(35): 22650-22661. https://doi.org/10.1039/D2RA04228B
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Fahima Aktar, Md. Ruhul, Sk. Omar Faruque, Farhana Rumi, Mohiuddin Abdul, Mohammad A. Rashid, Antioxidant, Cytotoxic, Membrane Stabilizing and Antimicrobial Activities of Bark and Seed of Entada phaseoloides (L.) Merr.: A Medicinal Plant from Chittagong Hill Tracts , Journal of Pharmacy and Nutrition Sciences : Vol. 1 No. 2 (2011)
- Sulaimon O. Folami, Georgina O. Erifeta, Mujidat O. Adepoju, Ibrahim Abuga, Muinat O. Kazeem, Abiodun S. Lawal, Abdullateef A. Alabi, Rodiat L. Mahmood, Kaotharat Yusuf Oganija, Phytochemical Study and Evaluation of Toxicity and Antioxidant Properties of Allium ascalonicum Leaf Extracts on Cell Line HepG2 , Journal of Pharmacy and Nutrition Sciences : Vol. 15 (2025)
- Adebowale Emmanuel Aladejana, Graeme Bradley, Anthony Jide Afolayan, Phytochemical Screening and In-Vitro Antioxidant Activities of Various Extracts of Helichrysum petiolare Hilliard & B.L. Burtt used for the Treatment of Diabetes Mellitus in the Eastern Cape Province of South Africa , Journal of Pharmacy and Nutrition Sciences : Vol. 10 No. 6 (2020)
- Mohammed Seid, Aman Dekebo, Neelaiah Babu, Phytochemical Investigation and Antimicrobial Evaluation of Foeniculum vulgare Leaves Extract Ingredient of Ethiopian Local Liquor , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 1 (2018)
- Alaa Zam , Mohammad Othman, Antioxidant Activity and other Physicochemical Characteristics of Different Propolis Extracts , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 2 (2018)
- Samadhan G. Patil, Bhushan S Bhadane, Mohini P. Patil, Sateesh Belemkar , Ravindra H. Patil, In-Vitro Antioxidant Activity, Acute Oral Toxicity Studies and Preliminary Phytochemical Characterization of the Bark Extract of Terminalia arjuna (L.) , Journal of Pharmacy and Nutrition Sciences : Vol. 6 No. 1 (2016)
- Fokhrul Abedin , Md. Saddam Hussain , Areful Islam , Niloy Sen , Abhijit Das , Auditi Kar , Md. Monir Hossain , Md. Shalahuddin Millat , Mohammad Safiqul Islam, Thrombolytic, CNS Depressant and Anti-Diarrhoeal Activities of Ethanolic Extract of Bark of Syzygium cumini L. Skeels: An In-Vivo and In-Vitro Study , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 3 (2018)
- Md. Sahab Uddin , G.M. Sala Uddin , Mst. Marium Begum , Yesmin Begum , Oscar Herrera-Calderon , Md. Manjurul Islam , Mohamed M. Abdel-Daim, Inspection of Phytochemical Content and In Vitro Antioxidant Profile of Gnaphalium luteoalbum L.: An Unexplored Phytomedicine , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 3 (2017)
- Chukwuemeka Paul Azubuike, Sandra Ebele Ejimba, Abel Olusola Idowu , Issac Adeleke, Formulation and Evaluation of Antimicrobial Activities of Herbal Cream Containing Ethanolic Extracts of Azadirachta indica Leaves and Aloe Vera Gel , Journal of Pharmacy and Nutrition Sciences : Vol. 5 No. 2 (2015)
- Anuck Islary, Jatin Sarmah , Sanjay Basumatary, Nutritional Properties, Phytochemicals and In Vitro Antioxidant Assessment of Two Wild Edible Fruits from Assam of North-East India , Journal of Pharmacy and Nutrition Sciences : Vol. 7 No. 2 (2017)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Pakorn Suksangsri, Weerapat Santinakul, Wiwit Suttithumsatid, Pharkphoom Panichayupakaranant, Integrated Green Microwave Extraction and Hydrophobic Resin-Based Enrichment of Mitragynine from Mitragyna speciosa , Journal of Pharmacy and Nutrition Sciences : Vol. 16 (2026)