logo

A Green Method for Preparation of Cyanidin-3-glucoside from Carissa carandas Fruits and α-Glucosidase Inhibitory Activity Evaluation

Authors

  • Kittituch Saengkhaw

    Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand

  • Nuttapong Arthan

    Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand

  • Pharkphoom Panichayupakaranant

    Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand and Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand

DOI:

https://doi.org/10.29169/1927-5951.2024.14.10

Keywords:

Antidiabetic, α- Glucosidase, Enriched extract, Green extraction, Carissa carandas, Cyanidin-3-glucoside

Abstract

In this research, a tailored approach for the preparation of a cyanidin-3-glucoside-enriched extract (C3GE) and cyanidin-3-glucoside (C3G) from the fruits of Carissa carandas L. was achieved using a green methodology. The method involved a cold extraction, followed by fractionation processes on a hydrophobic (Diaion® HP-20) column using a hydroethanolic solvent system for column elution. C3GE was produced after the one-step fractionation, while C3G was obtained after the two-step fractionation. Based on an HPLC method, C3GE contained 34.8% w/w of C3G, while C3G was identified via its 1H and 13C NMR data. An in vitro assay for the α-glucosidase inhibitory effect revealed that C3GE and C3G possessed good inhibitory activity against α-glucosidase, with IC50 values of 19.7 and 4.4 µg/mL, respectively, which is better than that of acarbose (IC50 of 395.4 µg/mL). Our findings suggest the potential use of this green extraction method for the production of C3G and C3GE, as well as its application in functional ingredient industries, including nutraceuticals and pharmaceuticals.

References

Anupama N, Madhumitha G, Rajesh KS. Role of dried fruits of Carissa carandas as anti-inflammatory agents and the analysis of phytochemical constituents by GC-MS. BioMed Research International 2014; 2014: 512369. https://doi.org/10.1155/2014/512369

Dhodi JB, Thanekar DR, Mestry SN, Juvekar AR. Carissa carandas Linn. fruit extract ameliorates gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress. J Acute Dis 2015; 4: 135-140. https://doi.org/10.1016/S2221-6189(15)30023-8

Singh R, Shrivastava M, Sharma P. Antidiabetic effect of Carissa carandas in rats and the possible mechanism of its insulin secretagogues activity in isolated pancreatic islets. J. Biomed Ther Sci 2019; 6: 1-7.

Lailerd M, Linn TW, Lailerd N, Amornlerdpison D, Imsumran A. Assessment of antidiabetic and anti-inflammatory activities of Carissa carandas Linn extract: In vitro and in vivo study. Appl Sci 2023; 13(11): 6454. https://doi.org/10.3390/app13116454

Mishra AB, Tomer V, Kumar A. Karonda (Carissa carandas L.): A miracle fruit with multifaceted potential. J Agric Food Res 2024; 18: 101417. https://doi.org/10.1016/j.jafr.2024.101417

Olivas-Aguirre FJ, Rodrigo-García J, Martínez-Ruiz ND, Cárdenas-Robles AI, Mendoza-Díaz SO, Álvarez-Parrilla E, González-Aguilar GA, de la Rosa LA, Ramos-Jiménez A, Wall-Medrano A. Cyanidin-3-O-glucoside: Physical-chemistry, foodomics and health effects. Molecules 2016; 21(9): 1264. https://doi.org/10.3390/molecules21091264

Cásedas G, Les F, González-Burgos E, Gómez-Serranillos MP, Smith C, López V. Cyanidin-3-O-glucoside inhibits different enzymes involved in central nervous system pathologies and type-2 diabetes. S Afr J Bot 2019; 120: 241-246. https://doi.org/10.1016/j.sajb.2018.07.001

Pan J, Zhang Q, Zhang C, Yang W, Liu H, Lv Z, Liu J, Jiao Z. Inhibition of dipeptidyl peptidase-4 by flavonoids: structure-activity relationship, kinetics and interaction mechanism. Front Nutr 2022; 9: 892426. https://doi.org/10.3389/fnut.2022.892426

Bartel I, Koszarska M, Strzałkowska N, Tzvetkov NT, Wang D, Horbańczuk JO, Wierzbicka A, Atanasov AG, Jóźwik A. Cyanidin-3-O-glucoside as a nutrigenomic factor in type 2 diabetes and its prominent impact on health. Int J Mol Sci 2023; 24(11): 9765. https://doi.org/10.3390/ijms24119765

Ye X, Chen W, Huang XF, Yan FJ, Deng SG, Zheng XD, Shan PF. Anti-diabetic effect of anthocyanin cyanidin-3-O-glucoside: data from insulin resistant hepatocyte and diabetic mouse. Nutr Diabetes 2024; 14(1): 1-8. https://doi.org/10.1038/s41387-024-00265-7

Chen L, Xin X, Lan R, Yuan Q, Wang X, Li Y. Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography. Food Chem 2014; 152: 386-90. https://doi.org/10.1016/j.foodchem.2013.11.080

Lee JS, Kim YR, Song IG, Ha SJ, Kim YE, Baek NI, Hong EK. Cyanidin-3-glucoside isolated from mulberry fruit protects pancreatic β-cells against oxidative stress-induced apoptosis. Int J Mol Med 2015; 35(2): 405-412. https://doi.org/10.3892/ijmm.2014.2013

Sasikumar R, Jyoti Das A, Chandra Deka S. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi J Biol Sci 2021; 28(9): 5338-5348. https://doi.org/10.1016/j.sjbs.2021.05.065

Xu Z, Liu W, Zhu L, Sun S, Xin X, Chen L, Liu Y. Preparative isolation and purification of three anthocyanins from red raspberry (Rubus ideaus L.) fruits by high-speed counter-current chromatography based on the optimization of the ultrasound-assisted extraction method. ACS Food Sci Technol 2022; 2(11): 1710-1718. https://doi.org/10.1021/acsfoodscitech.2c00215

Suttithumsatid W, Shah MA, Bibi S, Panichayupakaranant P. α-Glucosidase inhibitory activity of cannabidiol, tetrahydrocannabinol and standardized cannabinoid extracts from Cannabis sativa. Curr Res Food Sci 2022; 5: 1091-1097. https://doi.org/10.1016/j.crfs.2022.07.002

Oancea S. A Review of the current knowledge of thermal stability of anthocyanins and approaches to their stabilization to heat. Antioxidants (Basel) 2021; 10(9): 1337. https://doi.org/10.3390/antiox10091337

Panichayupakaranant, P. Active constituent-rich herbal extracts for development of phytomedicine. Songklanagarind Med J 2017; 35(3): 187-193. https://doi.org/10.31584/smj.2017.35.3.728

Shah MA, Reanmongkol W, Radenahmad N, Khalil R, Ul-Haq Z, Panichayupakaranant P. Anti-hyperglycemic and anti-hyperlipidemic effects of rhinacanthins-rich extract from Rhinacanthus nasutus leaves in nicotinamide-streptozotocin induced diabetic rats. Biomed Pharmacother 2019; 113: 108702. https://doi.org/10.1016/j.biopha.2019.108702

Meah MS, Panichayupakaranant P. α-Mangostin-rich extract: A potential oral antibacterial agent prepared by green extraction. Int J Pharmacogn Chinese Med 2020; 4: 000201. https://doi.org/10.23880/ipcm-16000201

Bhuyan P, Ganguly M, Baruah I, Borgohain G, Hazarika J, Sarma S. Alpha glucosidase inhibitory properties of a few bioactive compounds isolated from black rice bran: combined in vitro and in silico evidence supporting the antidiabetic effect of black rice. RSC Adv 2022; 12(35): 22650-22661. https://doi.org/10.1039/D2RA04228B

Downloads

Published

2024-11-14

Issue

Vol. 14 (2024)

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

A Green Method for Preparation of Cyanidin-3-glucoside from Carissa carandas Fruits and α-Glucosidase Inhibitory Activity Evaluation. (2024). Journal of Pharmacy and Nutrition Sciences , 14, 84-89. https://doi.org/10.29169/1927-5951.2024.14.10

Similar Articles

11-20 of 105

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)