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Chalcone Derived from a Natural Product: An Integrated Approach of Quantum Chemical Calculations, Molecular Docking, ADME and Neuromodulation on Serotonergic Receptors in Adult Zebrafish

Authors

  • Priscilla Teixeira da Silva

    Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil

  • Maria Kueirislene Amâncio Ferreira

    State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil

  • Roberto Lima de Albuquerque

    State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil

  • Alexandre Magno Rodrigues Teixeira

    State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil

  • Emmanuel Silva Marinho

    State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil

  • Márcia Machado Marinho

    State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil

  • Jesyka Macedo Guedes

    State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil

  • Marisa Jádna Silva Frederico

    Department of Pharmacology and Physiology, Center for Drug Research and Development, School of Medicine, Federal University of Ceará

  • Andreia Ferreira de Castro Gomes

    Center for Molecular and Environmental Biology, University of Minho, School of Sciences, Department of Biology, Braga, Portugal

  • Jane Eire Silva Alencar de Menezes

    State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil

  • Hélcio Silva dos Santos

    Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil; State University of Ceara, Graduate Program in Natural Sciences, Fortaleza, CE, Brazil and State University of Vale do Acaraú, Chemistry Course, Sobral, CE, Brazil

DOI:

https://doi.org/10.29169/1927-5951.2025.15.07

Keywords:

Chalcones, Anxiolytic effect, Serotonergic receptors, Zebrafish

Abstract

Anxiety disorders are conditions characterized by heightened responses to perceived threats, resulting in symptoms that negatively affect everyday life. This study investigates the anxiolytic effect of a natural chalcone isolated from Croton anisodontus Müll.Arg. focusing on its modulation of anxiolytic activity in modulating anxiolytic activity via GABAergic and serotonergic neurotransmission in an adult zebrafish model. The acute toxicity of the chalcone was assessed during a 96-hour period, and the anxiolytic behavior of fish treated with chalcone was evaluated using light/dark tests and open field tests (n=6 animals per group). Chalcone showed no signs of toxicity for up to 96 hours of analysis. The results demonstrated a significant anxiolytic effect of the synthesized chalcone, suggesting its therapeutic potential in treating anxiety. Furthermore, the findings indicate that this anxiolytic effect is mediated by serotonergic and non-GABAergic neurotransmitter systems. From molecular docking simulations, it was possible to estimate that the 5-HT3A receptor (5-HT3AR) pathway is the most likely target way for the chalcone to act. MPO-based ADME predictions indicate that the chalcone exhibits high cellular permeability suggest that chalcone has a high cellular permeability and can distribute better in biological tissues than in blood plasma, supporting its potential to act in the CNS by crossing the blood-brain barrier. These findings enhance the understanding of contribute to understanding of chalcone's mechanisms of action and provide a solid basis for future studies aimed at developing new therapeutic strategies to develop new therapeutic approaches for anxiety disorders.

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Published

2025-07-04

Issue

Vol. 15 (2025)

Section

Articles

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Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Chalcone Derived from a Natural Product: An Integrated Approach of Quantum Chemical Calculations, Molecular Docking, ADME and Neuromodulation on Serotonergic Receptors in Adult Zebrafish. (2025). Journal of Pharmacy and Nutrition Sciences , 15, 60-75. https://doi.org/10.29169/1927-5951.2025.15.07

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